基于微透析与微流控的新型肝素实时监测方法-《中国医学物理学杂志》

文章信息/Info

Title:: Real-time monitoring of heparin concentration using microdialysis-microfluidics system

作者:: 周星贝1; 2; 丁涛1; 王树水1; 2; 1.华南理工大学医学院，广东广州 510006； 2.广东省人民医院（广东省医学科学院）广东省心血管病研究所心儿科，广东广州 510080

Author(s):: ZHOU Xingbei1; 2; DING Tao1; WANG Shushui1; 2; 1. School of Medicine, South China University of Technology, Guangzhou 510006, China 2. Department of Pediatric Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Peoples Hospital (Guangdong Academy of Medical Sciences), Guangzhou 510080, China

Keywords:: Keywords: heparin microdialysis microfluidics real-time monitoring biochemical detection

摘要:: 目的：提供一种快速、连续、准确检测血液内肝素浓度及指导鱼精蛋白用药的新型诊断方法。方法：分别对微透析与微流控技术进行优化并整合，对人血浆中的肝素浓度变化进行实时监测。结果：该微透析-微流控系统能定量测量浓度范围为0~4.0 IU/mL的肝素，每隔30 s可自动读数1次，从取样到输出结果耗时16 min，结果与传统酶标仪测量方法的一致性高（R=0.995）。结论：该方法具有快速、准确、可完全覆盖肝素治疗浓度范围等特点，还可用于评价鱼精蛋白的用量，辅助临床用药。

Abstract:: Abstract: Objective To provide a novel method for detecting the heparin concentration in blood rapidly, continuously and accurately, and guiding the administration of protamine. Methods Both microdialysis technology and microfluidics technology were optimized and then integrated into a microdialysis-microfluidics system for the real-time monitoring of heparin concentration in human plasma. Results The microdialysis-microfluidics system could quantitatively measure the heparin within the concentration range of 0-4.0 IU/mL, with the function of automatic reading every 30 s. The duration from sampling to result output was 16 min. Moreover, the results output by the system had a high consistency with those obtained by the conventional microplate reader (R=0.995). Conclusion The proposed method can not only monitor the heparin concentration which was within therapeutic range rapidly and accurately, but also assess the dose of protamine, thereby guiding drug administration.