[1]李旭纲,陈一心,邱旭升.低能量脉冲电磁场对金黄色葡萄球菌生长的影响及其与抗生素的协同抑菌作用[J].中国医学物理学杂志,2021,38(10):1294-1298.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.020]
　LI Xugang,CHEN Yixin,QIU Xusheng.Effects of low-energy pulsed electromagnetic field on the growth of Staphylococcus aureus and its synergistic antibacterial effect with antibiotics[J].Chinese Journal of Medical Physics,2021,38(10):1294-1298.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.020]
点击复制

低能量脉冲电磁场对金黄色葡萄球菌生长的影响及其与抗生素的协同抑菌作用()
分享到：

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第10期
页码:
1294-1298
栏目:
医学生物物理
出版日期:
2021-10-27

文章信息/Info

Title:
Effects of low-energy pulsed electromagnetic field on the growth of Staphylococcus aureus and its synergistic antibacterial effect with antibiotics
文章编号:
1005-202X（2021）10-1294-05
作者:
李旭纲1; 陈一心1; 2; 邱旭升1; 2
1.南京中医药大学中西医结合鼓楼临床医学院， 江苏 南京 210008； 2.南京鼓楼医院骨科， 江苏 南京 210008
Author(s):
LI Xugang1;  CHEN Yixin1; 2;  QIU Xusheng1; 2
1. Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China 2. Department of Orthopaedics, Nanjing Drum Tower Hospital, Nanjing 210008, China
关键词:
金黄色葡萄球菌; 脉冲电磁场; 抗生素; 协同抑菌作用
Keywords:
Keywords: Staphylococcus aureus pulsed electromagnetic fields antibiotics synergistic antibacterial effect
分类号:
R37
DOI:
DOI:10.3969/j.issn.1005-202X.2021.10.020
文献标志码:
A
摘要:
目的：探讨低能量脉冲电磁场（PEMF）对体外金黄色葡萄球菌（ATCC 29213）生长的影响及其与抗生素是否有协同作用。方法：实验分两部分。（1）分析不同作用时长的PEMF对金黄色葡萄球菌生长的影响。将一定量金黄色葡萄球菌接种至普通营养肉汤，分为对照组和PEMF干预组（场强1 Gs，频率15 Hz，脉宽5 μs，脉冲群宽5 ms，脉冲群不应期62 ms），两组均置于37 ℃温箱培养，每隔2 h取样倍比稀释后接种至哥伦比亚血平板，37℃温箱培养，24 h后计数。（2）分析PEMF与抗生素对金黄色葡萄球菌的联合影响。分为对照组、PEMF组、抗生素组、PEMF+抗生素组；4组均置于37 ℃温箱培养，8 h后取样倍比稀释并接种至哥伦比亚血平板，37 ℃温箱培养，24 h后计数。结果：（1）2 h、4 h的PEMF干预与对照组比较，菌落计数差异无统计学意义（P>0.05）；6 h的PEMF干预后，菌落计数为对照组的23%（P<0.01）；8 h的PEMF干预后，菌落计数为对照组的28%（P=0.01）。（2）PEMF组、抗生素组、PEMF+抗生素组菌落计数分别是对照组的32%、34%，10%，都显著少于对照组；同时，PEMF+抗生素组菌落计数显著少于PEMF组和抗生素组。结论：低能量PEMF可显著抑制对数生长期金黄色葡萄球菌，同时与抗生素之间具有协同抑菌作用。
Abstract:
Abstract: Objective To analyze the effect of low-energy pulsed electromagnetic field (PEMF) on the growth of Staphylococcus aureus (ATCC 29213) in vitro, and to investigate whether low-energy PEMF has synergistic antibacterial effect with antibiotics. Methods The experiment was divided into two parts. (1) Analyzing the effect of PEMF with different action durations on the growth of Staphylococcus aureus. A certain amount of Staphylococcus aureus was inoculated into ordinary nutrition broth, and then divided into control group and PEMF intervention group in which Staphylococcus aureus was intervened with PEMF with a field strength of 1 Gs, frequency of 15 Hz, pulse width of 5 μs, pulse group width of 5 ms and pulse group refractory period of 62 ms. Both two groups were cultured in 37 ℃ incubator. The samples were collected every 2 hours, and after dilution, they were inoculated into Columbia blood plate in 37 ℃ incubator and counted after 24 hours. (2) Analyzing the synergistic antibacterial effect of PEMF and antibiotics on Staphylococcus aureus. The Staphylococcus aureus was divided into 4 groups, namely control group, PEMF group, antibiotics group, and PEMF+antibiotics group. The 4 groups were cultured in a 37°C incubator. After 8 hours, the samples were diluted and inoculated into Columbia blood plates in 37°C incubator and counted after 24 hours. Results (1) There was no significant difference in colony count between PEMF interventions for 2 and 4 hours and control group (P>0.05). After PEMF intervention for 6 hours, the colony count in PEMF group was 23% of control group (P<0.01), and after PEMF intervention for 8 hours, the colony count in PEMF group was 28% of control group (P<0.01). (2) The colony count of PEMF group, antibiotics group and PEMF+antibiotics group were 32%, 34% and 10% of control group, respectively, which were significantly less than that of control group. Meanwhile, the colony count of PEMF+antibiotics group was significantly less than that of PEMF group and antibiotics group. Conclusion The low-energy PEMF can significantly inhibit Staphylococcus aureus in the logarithmic growth period, and has a synergistic antibacterial effect with antibiotics.

相似文献/References:

[1]焦明克,胡劼,焦琳,等.低频脉冲电磁场对冻伤组织血液循环的影响[J].中国医学物理学杂志,2013,30(01):3946.[doi:10.3969/j.issn.1005-202X.2013.01.028]
[2]周鑫,邓修元,庞剑飞,等.不同放电方式等离子体的灭菌效果研究[J].中国医学物理学杂志,2013,30(04):4316.[doi:10.3969/j.issn.1005-202X.2013.04.023]
[3]赵承奇,雷涛,罗二平,等.脉冲电磁场对糖尿病大鼠外周神经病变的影响[J].中国医学物理学杂志,2013,30(05):4431.[doi:10.3969/j.issn.1005-202X.2013.05.021]
[4]张敏,李维娜,高贺奇,等.脉冲电磁场对大鼠骨髓间充质干细胞活性的影响[J].中国医学物理学杂志,2015,32(06):777.[doi:doi:10.3969/j.issn.1005-202X.2015.06.004]
　[J].Chinese Journal of Medical Physics,2015,32(10):777.[doi:doi:10.3969/j.issn.1005-202X.2015.06.004]
[5]陆彦邑,毕玉田,尹军,等. 基于高场不对称波形离子迁移谱技术的细菌培养液检测[J].中国医学物理学杂志,2017,34(3):297.[doi:10.3969/j.issn.1005-202X.2017.03.016]
　[J].Chinese Journal of Medical Physics,2017,34(10):297.[doi:10.3969/j.issn.1005-202X.2017.03.016]
[6]翟明明,刘娟,罗二平,等.脉冲电磁场对模拟高原环境小鼠肝脏氧化应激的影响[J].中国医学物理学杂志,2019,36(11):1330.[doi:DOI:10.3969/j.issn.1005-202X.2019.11.016]
　ZHAI Mingming,LIU Juan,LUO Erping,et al.Effects of pulsed electromagnetic field on hepatic oxidative stress of mice in simulated high-altitude environment[J].Chinese Journal of Medical Physics,2019,36(10):1330.[doi:DOI:10.3969/j.issn.1005-202X.2019.11.016]
[7]柏中喜,方兴刚,马龙祥,等.脉冲电磁场通过Wntβ-catenin信号通路改善膝骨关节炎大鼠炎症反应的机制[J].中国医学物理学杂志,2022,39(5):617.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.016]
　BAI Zhongxi,FANG Xinggang,MA Longxiang,et al.Mechanism of pulsed electromagnetic field in inhibiting inflammatory response in knee osteoarthritis rats through Wntβ-catenin signaling pathway[J].Chinese Journal of Medical Physics,2022,39(10):617.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.016]

备注/Memo

备注/Memo:
【收稿日期】2021-05-20 【基金项目】江苏省青年医学人才项目（QNRC2016009） 【作者简介】李旭纲，硕士研究生，研究方向：创伤骨科，E-mail: lixugang1995@126.com 【通信作者】邱旭升，博士，副主任医师，研究方向：创伤骨科，E-mail: qiu_xusheng@163.com
更新日期/Last Update: 2021-10-29