[1]朱怡卿,王芳. 脂质体姜黄素联合索拉非尼对人肝癌Huh7细胞的抑制作用[J].中国医学物理学杂志,2018,35(11):1324-1328.[doi:DOI:10.3969/j.issn.1005-202X.2018.11.016]
　ZHU Yiqing,WANG Fang. Inhibitory effects of liposome curcumin combined with sorafenib on human hepatocellular carcinoma Huh7 cells[J].Chinese Journal of Medical Physics,2018,35(11):1324-1328.[doi:DOI:10.3969/j.issn.1005-202X.2018.11.016]
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 脂质体姜黄素联合索拉非尼对人肝癌Huh7细胞的抑制作用()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
35卷
期数:
2018年第11期
页码:
1324-1328
栏目:
医学生物物理
出版日期:
2018-11-18

文章信息/Info

Title:
 Inhibitory effects of liposome curcumin combined with sorafenib on human hepatocellular carcinoma Huh7 cells
文章编号:
1005-202X（2018）11-1324-05
作者:
 朱怡卿; 王芳
 第二军医大学医学遗传学教研室， 上海 200433
Author(s):
 ZHU Yiqing;  WANG Fang
 Department of Medical Genetics, the Second Military Medical University, Shanghai 200433, China
关键词:
 ; 肝癌; 脂质体姜黄素; 索拉非尼; 联合用药
Keywords:
 ; Keywords: liver cancer;  liposomes curcumin;  sorafenib;  combination therapy
分类号:
R392；R318
DOI:
DOI:10.3969/j.issn.1005-202X.2018.11.016
文献标志码:
A
摘要:
 【摘 要】 目的：探讨脂质体姜黄素联合索拉非尼对肝癌Huh7细胞的抑制作用。 方法：采用脂质体包埋技术制备脂质体姜黄素，并通过Nano-ZS激光粒度测定仪测定其粒径大小及Zeta电位。分别用浓度为10 ?mol/L脂质体姜黄素、0.1 ?mol/L索拉非尼单独及联合处理人肝癌Huh7细胞，应用CCK8检测和EdU检测评价其对Huh7细胞增殖的影响，通过Transwell小室迁移实验检测其对Huh7细胞迁移能力的影响。 结果：获得粒径为（118.7±13.6） nm、Zeta电位为（-9.8±1.1） mV的脂质体姜黄素，脂质体姜黄素单独使用可显著抑制Huh7细胞的增殖和迁移，脂质体姜黄素与索拉非尼联合使用可显著提高索拉非尼对Huh7细胞增殖与迁移的抑制能力。 结论：脂质体姜黄素与索拉非尼联合使用可显著抑制肝癌Huh7细胞的增殖和迁移，是肝癌治疗的一种潜在策略。
Abstract:
 Abstract: Objective To investigate the inhibition of the combination therapy of liposome curcumin and sorafenib on hepatocellular carcinoma Huh7 cells. Methods Liposome curcumin was prepared by liposome embedding technique, and its particle size and Zeta potential were determined by Nano-ZS laser particle size analyzer. Human hepatocellular carcinoma Huh7 cells were treated with liposome curcumin (10 ?mol/L) and sorafenib (0.1 ?mol/L) separately and jointly. The effects of liposome curcumin and sorafenib on the proliferation of Huh7 cells were evaluated with CCK8 assay and EdU assay, and their effects on the migration of Huh7 cells were detected by Transwell chamber migration assay. Results Liposome curcumin with a diameter of (118.7±13.6) nm and Zeta potential of (-9.8±1.1) mV was obtained. Using liposome curcumin alone significantly inhibited the proliferation and migration of Huh7 cells. The combined use of liposomal curcumin and sorafenib remarkably improved the inhibitory effects of sorafenib on the proliferation and migration of Huh7 cells. Conclusion The combined use of liposome curcumin and sorafenib which can inhibit the proliferation and migration of hepatoma Huh7 cells is a potential strategy for the treatment of hepatocellular carcinoma.

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备注/Memo

备注/Memo:
 【收稿日期】2018-10-15
【基金项目】国家自然科学基金面上项目（81672775）；973国家重点基础研究发展计划（2015CB554004）
【作者简介】朱怡卿，硕士研究生，研究方向：肿瘤表观遗传学，肿瘤治疗，E-mail: 971899787@qq.com
【通信作者】王芳，博士，教授，博士生导师，研究方向：肿瘤表观遗传学，肿瘤免疫治疗，E-mail: baichongch@163.com
更新日期/Last Update: 2018-11-22