[1]翟明明,刘娟,罗二平,等.脉冲电磁场对模拟高原环境小鼠肝脏氧化应激的影响[J].中国医学物理学杂志,2019,36(11):1330-1334.[doi:DOI:10.3969/j.issn.1005-202X.2019.11.016]
　ZHAI Mingming,LIU Juan,LUO Erping,et al.Effects of pulsed electromagnetic field on hepatic oxidative stress of mice in simulated high-altitude environment[J].Chinese Journal of Medical Physics,2019,36(11):1330-1334.[doi:DOI:10.3969/j.issn.1005-202X.2019.11.016]
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脉冲电磁场对模拟高原环境小鼠肝脏氧化应激的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
36卷
期数:
2019年第11期
页码:
1330-1334
栏目:
医学生物物理
出版日期:
2019-11-25

文章信息/Info

Title:
Effects of pulsed electromagnetic field on hepatic oxidative stress of mice in simulated high-altitude environment
文章编号:
1005-202X（2019）11-1330-05
作者:
翟明明; 刘娟; 罗二平; 汤池
空军军医大学生物医学工程系， 陕西 西安 710032
Author(s):
ZHAI Mingming;  LIU Juan;  LUO Erping;  TANG Chi
School of Biomedical Engineering, Air Force Medical University, Xi’an 710032, China
关键词:
肝脏; 氧化应激; 脉冲电磁场; 模拟高原环境
Keywords:
liver;  oxidative stress;  pulsed electromagnetic field;  simulated high-altitude environment
分类号:
R318.08
DOI:
DOI:10.3969/j.issn.1005-202X.2019.11.016
文献标志码:
A
摘要:
目的：探究脉冲电磁场对模拟高原环境下小鼠肝脏氧化应激的影响。方法：将15只雄性SPF级小鼠平均随机分为平原对照组（Control组）、低压低氧组（HH组）和脉冲电磁场干预组（PEMF组），HH组和PEMF组置于低压低氧舱内模拟海拔6 000 m，构建小鼠肝脏氧化应激模型。其中PEMF组每天施加2 mT磁场干预2 h。实验进行5 d后处死小鼠取材，对肝组织的氧化应激生化指标进行检测，并对抗氧化酶活性蛋白表达进行检测。结果：与HH组相比较，PEMF组丙二醛和氧化型谷胱甘肽水平显著降低，而还原谷胱甘肽水平显著上升；与HH组比较，PEMF组谷胱甘肽过氧化物酶活性显著上升，与蛋白表达活性相一致。结论：脉冲电磁场可以改善模拟高原环境引起的小鼠肝脏氧化应激失衡，显著提高谷胱甘肽过氧化物酶活性。
Abstract:
Objective To investigate the effects of pulsed electromagnetic field on the hepatic oxidative stress of mice in simulated high-altitude environment. Methods Fifteen male SPF mice were randomly divided into plain control group (Control group), hypobaric hypoxia group (HH group) and pulsed electromagnetic field intervention group (PEMF group). The mice in HH and PEMF groups were set in a low pressure and low oxygen cabin which was used to simulate an altitude of 6 000 m, so as to construct a mouse model of hepatic oxidative stress. The mice in PEMF group were treated with 2 mT magnetic field per day for 2 h. All mice were sacrificed at 5 days after the experiment, and samples were collected. The biomarkers of oxidative stress in hepatic tissues were detected, and the expression of antioxidant enzymes active protein was detected. Results Compared with those in HH group, the levels of methane dicarboxylic aldehyde and oxidized glutathione in PEMF group were reduced obviously, while the level of reduced glutathione was increased significantly, and the glutathione peroxidase activity in PEMF group was increased dramatically, which was consistent with protein expression activity. Conclusion Pulsed electromagnetic field can improve the imbalance of hepatic oxidative stress in mice which was caused by simulated high-altitude environment, and significantly increase the activity of glutathione peroxidase.

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备注/Memo

备注/Memo:
【收稿日期】2019-09-25 【基金项目】国家自然科学基金（81471806）；陕西省重点研发一般项目（2018SF-176） 【作者简介】翟明明，博士，讲师，研究方向：高原抗缺氧，E-mail: zhai-mingming@fmmu.edu.cn 【通信作者】汤池，博士，副教授，研究方向：高原抗缺氧，E-mail: tangchi@fmmu.edu.cn
更新日期/Last Update: 2019-11-28