Effects of low-energy pulsed electromagnetic field on the growth of Staphylococcus aureus and its synergistic antibacterial effect with antibiotics(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第10期
- Page:
- 1294-1298
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of low-energy pulsed electromagnetic field on the growth of Staphylococcus aureus and its synergistic antibacterial effect with antibiotics
- Author(s):
- LI Xugang1; CHEN Yixin1; 2; QIU Xusheng1; 2
- 1. Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China 2. Department of Orthopaedics, Nanjing Drum Tower Hospital, Nanjing 210008, China
- Keywords:
- Keywords: Staphylococcus aureus pulsed electromagnetic fields antibiotics synergistic antibacterial effect
- PACS:
- R37
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.10.020
- Abstract:
- Abstract: Objective To analyze the effect of low-energy pulsed electromagnetic field (PEMF) on the growth of Staphylococcus aureus (ATCC 29213) in vitro, and to investigate whether low-energy PEMF has synergistic antibacterial effect with antibiotics. Methods The experiment was divided into two parts. (1) Analyzing the effect of PEMF with different action durations on the growth of Staphylococcus aureus. A certain amount of Staphylococcus aureus was inoculated into ordinary nutrition broth, and then divided into control group and PEMF intervention group in which Staphylococcus aureus was intervened with PEMF with a field strength of 1 Gs, frequency of 15 Hz, pulse width of 5 μs, pulse group width of 5 ms and pulse group refractory period of 62 ms. Both two groups were cultured in 37 ℃ incubator. The samples were collected every 2 hours, and after dilution, they were inoculated into Columbia blood plate in 37 ℃ incubator and counted after 24 hours. (2) Analyzing the synergistic antibacterial effect of PEMF and antibiotics on Staphylococcus aureus. The Staphylococcus aureus was divided into 4 groups, namely control group, PEMF group, antibiotics group, and PEMF+antibiotics group. The 4 groups were cultured in a 37°C incubator. After 8 hours, the samples were diluted and inoculated into Columbia blood plates in 37°C incubator and counted after 24 hours. Results (1) There was no significant difference in colony count between PEMF interventions for 2 and 4 hours and control group (P>0.05). After PEMF intervention for 6 hours, the colony count in PEMF group was 23% of control group (P<0.01), and after PEMF intervention for 8 hours, the colony count in PEMF group was 28% of control group (P<0.01). (2) The colony count of PEMF group, antibiotics group and PEMF+antibiotics group were 32%, 34% and 10% of control group, respectively, which were significantly less than that of control group. Meanwhile, the colony count of PEMF+antibiotics group was significantly less than that of PEMF group and antibiotics group. Conclusion The low-energy PEMF can significantly inhibit Staphylococcus aureus in the logarithmic growth period, and has a synergistic antibacterial effect with antibiotics.
Last Update: 2021-10-29