Dose-effect relationship between shock wave intensity and blast lung injury in juvenile rats(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第6期
- Page:
- 780-784
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Dose-effect relationship between shock wave intensity and blast lung injury in juvenile rats
- Author(s):
- YUAN Danfeng; YANG Ao; MA Chao; LIANG Yi; DAI Wei; ZHOU Jihong
- Institute of Field Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China
- Keywords:
- Keywords: blast lung injury juvenile rat shock wave dose-effect relationship
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.06.022
- Abstract:
- Abstract: Objective To explore the dose-effect relationship between shock wave intensity and blast lung injury in juvenile rats, thereby providing animal model and research foundation for the study on blast injury in children. Methods A total of 40 healthy SD male rats of 20 days old were randomly divided into 4 groups, namely BIG1, BIG2, BIG3 and BIG4, with 10 in each group. The rats were injured by BST-I bioshock tube with actuating pressures at 4.8 MPa to 5.8 MPa. The vital signs after injury as well as the gross anatomy and light microscope pathology of the lungs in each group were analyzed, and then the severity of blast lung injury was scored. Results All the juvenile rats showed different degrees of shortness of breath and heart rate increasing after injury, and the incidence of external auditory canal hemorrhage was 57.5% (46/80). The gross anatomy of the lungs was characterized by various degrees of lung hemorrhage, edema, atelectasis and so on. The pathological changes observed by light microscopy mainly included different degrees of lung hemorrhage, effusion, inflammatory cells infiltration, thickening and edema of pulmonary mesenchyme, alveolar edema, alveolar wall rupture and so on. At an actuating pressure of 4.8 MPa, the peak value of overpressure on the animal was 433 kPa the positive impulse was 14 226.4 kPa[?m organ injury scaling (OIS) was concentrated in grade II and III (40%, 30%) abbreviated injury scale (AIS) score was 0.90±0.57 and it was finally diagnosed as mild lung injury. When the actuating pressure was 5.0 MPa, peak overpressure and positive impulse were 447.7 kPa and 14 463.5 kPa[?m, respectively and the lung injury was concentrated in OIS grade III (60%), with an AIS score of 1.60±0.69 and it was finally diagnosed as moderate lung injury. When the actuating pressure was 5.5 MPa, peak overpressure and positive impulse were 484.7 kPa and 15 017.0 kPa[?m, respectively and the lung injury was concentrated in OIS grade IV (70%), with an AIS score of 3.10±0.56 and it was finally diagnosed as relatively severe lung injury. When actuating pressure was 5.8 MPa, peak overpressure and positive impulse were 506.8 kPa and 15 325.5 kPa[?m, respectively the lung injury was concentrated near OIS grade V (40%), with an AIS score of 4.00±0.67 and it was finally diagnosed as severe lung injury. Statistically significant difference was found in the severity of lung injury among different groups (P<0.05). Conclusion A stable model of mild-to-severe blast lung injury in juvenile SD rats can be established using BST-I bioshock tube with actuating pressures at 4.8 MPa to 5.8 MPa. The lung tissues of juvenile rats are more tolerant to blast injury than lung tissues of adult rats and juvenile rabbits, while the mechanism is still unclear and deserves further research.
Last Update: 2021-06-29