Magnetic resonance imaging of angiogenesis and lymphangiogenesis in tumor using dual-targeted CL-PEG-MnFe2O4 nanomicelles(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第9期
- Page:
- 1034-1038
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Magnetic resonance imaging of angiogenesis and lymphangiogenesis in tumor using dual-targeted CL-PEG-MnFe2O4 nanomicelles
- Author(s):
- YANG Hua1; GONG Mingfu2; ZOU Liguang2; ZENG Guofei1; FANG Yu1; LIU Cuifang1
- 1. Department of Radiology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, China; 2. Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
- Keywords:
- Keywords: tumor; endothelial cell; CL-PEG-MnFe2O4 nanomicelles; molecular imaging; magnetic resonance
- PACS:
- R318.08；R814
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.09.008
- Abstract:
- Abstract: Objective To evaluate the value of Endoglin targeted CL-PEG-MnFe2O4 nanomicelles in magnetic resonance (MR) imaging of tumor microvessels and lymphatic microvessels. Methods In 5 nude-mice breast cancer xenografts models, a total of 0.15 mL CL-PEG-MnFe2O4 nanomicelles with a concentration of 50 μg/mL was intravenously administrated by retrobulbar vein. MR imaging was performed at 0, 5, 15, 30, 60, 120 min after contrast injection. The MR imaging sequences included spin echo T1-weighted imaging (SE T1WI), fast spin echo T2-weighted imaging (FSE T2WI), gradient echo T2*-weighted imaging (GRE T2*WI) and T2 mapping. The tumor signal intensity was measured at each time point, and time-intensity curve was plotted to analyze the change rule of the curve. The T2 and R2 values of the tumor were measured and calculated, so as to evaluate the target enhancement performance of CL-PEG-MnFe2O4 nanomicelles. Non-targeted PEG-PCL-MnFe2O4 was served as a control. Results In the early period after the intravenous administration of nanomicelles, the tumor was negatively enhanced and the peripheral area of the tumor had obvious enhancement. At 60 min after the intravenous administration of targeted CL-PEG-MnFe2O4 nanomicelles, the tumor displayed patchy or spot-like enhancement, which was mainly limited to the peripheral areas of the tumor. However, the tumor signal intensity returned to the baseline at 60 min after the administration of non-targeted PEG-PCL-MnFe2O4 nanomicelles. The T2 values of the tumor before and at 0, 5, 15, 30, 60 and 120 min after the administration of CL-PEG-MnFe2O4 nanomicelles were (77.98±10.29), (44.66±5.25), (50.80±3.85), (54.25±5.08), (57.20±4.04), (59.20±7.11) and (60.15±7.43) ms, respectively; and those values before and after the administration of PEG-PCL-MnFe2O4 nanomicelles were (78.66±5.71), (44.85±5.67), (50.06±8.62), (63.10±8.36), (70.19±7.71), (74.76±10.60) and (76.63±12.13) ms, respectively. After the intravenous administration of CL-PEG-MnFe2O4 nanomicelles, the time-intensity curve was decreased firstly and then increased and finally reached a plateau, while the time-intensity curve after the intravenous administration of PEG-PCL-MnFe2O4 was decrease firstly and then increased. Conclusion Endoglin targeted CL-PEG-MnFe2O4 nanomicelles can bind to tumor microvessels and lymphatic microvessels, which can be detected by MR imaging, thereby providing an experimental basis for targeted MR imaging of tumor microvessels and lymphatic microvessels.
Last Update: 2019-09-23