Effects of different single doses of wltraviolet B on cornea of rats(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2023年第4期
- Page:
- 473-479
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of different single doses of wltraviolet B on cornea of rats
- Author(s):
- WANG Tingting; HUANG Qiaohong; LIN Xinxin; YANG Qian; ZHU Jingyu; HUANG Yan
- Department of Ophthalmology and Optometry, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China
- Keywords:
- Keywords: ultraviolet irradiation dose cornea aldehyde dehydrogenase
- PACS:
- R772.2
- DOI:
- DOI:10.3969/j.issn.1005-202X.2023.04.013
- Abstract:
- Abstract: Objective To explore the effects of different single doses of ultraviolet B (UVB) on the structure and function of rat cornea under the condition that the total dose of UVB irradiation is constant. Methods Forty healthy SD rats were randomly divided into 4 groups (10 rats in each group). The rats in control group were raised under natural light, and the samples were taken on day 42. The daily UVB irradiation intensity in experimental groups remained at 146 μW/cm? and each group had the same cumulative irradiation dose. The rats in single low-, medium- and high-dose groups were exposed to daily UVB irradiation for 12, 24 and 48 min, respectively. On the 84th, 42nd and 21st days of continuous irradiation, the samples were collected for slit-lamp examination, corneal fluorescein sodium staining, and tear film break-up time (BUT) test. TUNEL was adopted to detect corneal cell apoptosis, and ELISA was used to detect the activity and expression of aldehyde dehydrogenase (ALDH). Results The rats in control group maintain corneal transparence, while in experimental groups, with the increase of the single irradiation dose, the degree of corneal opacity was aggravated. The corneal fluorescein sodium staining scores in control group and experimental groups were 0, (4.30±1.42), (8.90±1.73), and (11.10±1.97), respectively. Compared with control group, experimental groups had shorter BUT (P<0.05) which was shortened with the increase of the single irradiation dose, and higher apoptosis index (P<0.05) which was increased with the increase of single irradiation dose. There was no significant difference in corneal thickness between single low-dose group and control group (P>0.05), but the corneal thickness were thinner in single medium- and high-dose groups than in control group (P<0.05). The activity and expression of corneal ALDH in experimental groups were lower than those in control group (P<0.05), and the decrease was more significant with the increase of single irradiation dose. Conclusion When the total dose of UVB irradiation is constant, single high dose of UVB irradiation results in corneal opacity and corneal thinning in rats and may induce keratoconus. These damages are related to the decreased antioxidant capacity caused by the decreased activity and expression of ALDH, which suggests that it can protect the cornea and reduce UV damage by increasing the activity of ALDH.
Last Update: 2023-04-25