Abstract

Info

Author(s):: SUN Fuqi1; SA Yu1; LI Qifeng1; FENG Jingwen2; HAN Yangguang1; 1. School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin 300072, China 2. Department of Radiation Oncology, Tianjin Huanhu Hospital, Tianjin 300222, China

Keywords:: Keywords: flow cytometer cell sorting and enrichment lable-free microfluidic chip review

Abstract:: Abstract: Flow cytometry has always been an essential means for biological cell research. In recent years, the applications of flow cytometer for cell enrichment and post-screening reuse have led to an increased demand for flow cytometers capable of high throughput, high cell information extraction as well as high cell activity. Herein the structure, working principle and current development status of flow cytometer based on optical detection methods are overviewed. In order to meet the new application demands and overcome the inherent defects of the current commercial optical flow cytometer, the trends of label-free and microfluidics chip emerge in optical flow cytometer. The emerging technologies such as label-free imaging methods, microfluidics on-chip sorting systems, and on-chip piezoelectric actuator integrated sorting are increasingly applied to the research and design of optical flow cytometer. The research advances and challenges of these technologies are summarized from the aspects of technical characteristics, performance advantages, application prospects, etc., for providing users and researchers with reference.