[1]张珂,张陶冶,黄慧明.基于明胶膜基底捕获和原位培养循环肿瘤细胞[J].中国医学物理学杂志,2021,38(12):1549-1553.[doi:DOI:10.3969/j.issn.1005-202X.2021.12.017]
 ZHANG Ke,ZHANG Taoye,HUANG Huiming.Capture and culture in situ of circulating tumor cells based on gelatin film substrate[J].Chinese Journal of Medical Physics,2021,38(12):1549-1553.[doi:DOI:10.3969/j.issn.1005-202X.2021.12.017]
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基于明胶膜基底捕获和原位培养循环肿瘤细胞()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第12期
页码:
1549-1553
栏目:
医学生物物理
出版日期:
2021-12-24

文章信息/Info

Title:
Capture and culture in situ of circulating tumor cells based on gelatin film substrate
文章编号:
1005-202X(2021)12-1549-05
作者:
张珂张陶冶黄慧明
武汉大学物理科学与技术学院, 湖北 武汉 430072
Author(s):
ZHANG Ke ZHANG Taoye HUANG Huiming
School of Physics and Technology, Wuhan University, Wuhan 430072, China
关键词:
循环肿瘤细胞明胶膜基底原位培养
Keywords:
circulating tumor cells gelatin film substrate culture in situ
分类号:
R-33
DOI:
DOI:10.3969/j.issn.1005-202X.2021.12.017
文献标志码:
A
摘要:
目前从血液中分离得到高活力的循环肿瘤细胞(CTCs)仍面临一定的挑战,本研究设计了一个明胶膜基底,可以同时实现捕获和原位培养CTCs。该明胶基底对CTCs的捕获效率最高可达86.8%。由于明胶具有良好的生物相容性,明胶膜基底在捕获到CTCs后可直接进行原位培养,减少了释放过程中对细胞的损害,有利于后续细胞分析。该明胶膜基底有望在临床CTCs检测中发挥作用。
Abstract:
At present, there are some challenges in obtaining circulating tumor cells (CTCs) with high viability from blood. Herein a gelatin film substrate is designed for realizing the capture and culture in situ of CTCs. The maximum capture efficiency of the proposed substrate for CTCs reaches 86.8%. Because of the good biocompatibility of gelatin, CTCs can be directly cultured in situ after being captured by gelatin film substrate, which reduces the damages to the cells during the release process and is more beneficial for subsequent cellular analysis. The gelatin film substrate is expected to play a role in the clinical detection of CTCs.

相似文献/References:

[1]颜识涵,姚春艳. 太赫兹光谱技术挑战检测循环肿瘤细胞难题[J].中国医学物理学杂志,2016,33(12):1225.[doi:10.3969/j.issn.1005-202X.2016.12.009]
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[2]王正源,徐秀林,王燕. 基于微流体技术分选循环肿瘤细胞的方法研究[J].中国医学物理学杂志,2019,36(2):229.[doi:DOI:10.3969/j.issn.1005-202X.2019.02.020]
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备注/Memo

备注/Memo:
【收稿日期】2021-06-15 【基金项目】武汉应用基金会前沿项目(2019010701011386);深圳市知识创新计划基础研究项目(JCYJ20180302173424902) 【作者简介】张珂,硕士在读,研究方向:循环肿瘤细胞的捕获和鉴定,E-mail: 2018282020096@whu.edu.cn 【通信作者】黄慧明,副教授,硕士生导师,研究方向:微流控芯片、循环肿瘤细胞捕获与鉴定,E-mail: huiming1918@sina.com
更新日期/Last Update: 2021-12-24