钆塞酸二钠增强磁共振成像评估小鼠脑缺血再灌注损伤中炎症动态变化-《中国医学物理学杂志》

文章信息/Info

Title:: Dynamic assessment of inflammation in mice with cerebral ischemia-reperfusion injury using gadoxetate disodium-enhanced magnetic resonance imaging

Author(s):: ZHAO Yiling; RUAN Peng; HE Li; LUO Shiyong; ZHOU Lang; YANG Fan; Department of Radiology, Wuhan Third Hospital, Wuhan 430074, China

Keywords:: Keywords: cerebral ischemia-reperfusion disodium gadoxetate inflammatory response magnetic resonance

摘要:: 目的：利用钆塞酸二钠增强磁共振成像研究小鼠脑缺血再灌注损伤炎症反应的动态变化。方法：通过封闭缝合线阻断右侧大脑中动脉，建立C57BL/6n小鼠脑缺血再灌注模型。随后，小鼠尾静脉注射钆塞酸二钠，在相应时间点进行磁共振扫描，观察信号变化。此外，免疫组织化学技术用于测量脑组织炎症因子的水平。结果：在大脑中动脉闭塞（MCAO）组和MCAO+钆塞酸二钠组之间，脑组织炎症因子肿瘤坏死因子-α（TNF-α）和白细胞介素1β（IL-1β）水平无显著性差异。在注射钆塞酸二钠的小鼠再灌注后负增强区的最低信号比降低。结论：钆塞酸二钠注射液不影响脑缺血再灌注急性期的炎症反应，钆塞酸二钠增强磁共振成像可作为监测脑缺血再灌注损伤急性期炎症反应的有效手段。

Abstract:: Abstract: Objective To investigate the dynamic changes of inflammatory responses in mice with cerebral ischemia-reperfusion injury using gadoxetate disodium-enhanced magnetic resonance imaging. Methods A C57BL/6n mouse model of cerebral ischemia-reperfusion was established by blocking the right middle cerebral artery with the closed suture. Subsequently, gadoxetate disodium was injected into the tail vein of the mice, and MRI scans were performed at the corresponding time points to observe the signal changes. In addition, immunohistochemical techniques were used to measure the levels of inflammatory cytokines in brain tissues. Results There were no significant differences in the levels of tumor necrosis factor-α and interleukin-1β between the middle cerebral artery occlusion (MCAO) group and MCAO + gadoxetate disodium group. The lowest signal ratio in the negative enhancement region was reduced after reperfusion in mice injected with disodium gadolinium. Conclusion Gadoxetate disodium injection does not affect the inflammatory response during acute cerebral ischemia-reperfusion. Therefore, gadoxetate disodium-enhanced magnetic resonance imaging can be used as an effective means to monitor the inflammatory response during acute cerebral ischemia-reperfusion.