[1]郭冰冰,胡秀枋,宋子玥,等.循环肿瘤细胞检测仪的结构设计与功能分析[J].中国医学物理学杂志,2022,39(5):604-610.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.014]
　GUO Bingbing,HU Xiufang,SONG Ziyue,et al.Structural design and functional analysis of an apparatus for detection of circulating tumor cells[J].Chinese Journal of Medical Physics,2022,39(5):604-610.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.014]
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循环肿瘤细胞检测仪的结构设计与功能分析()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
39卷
期数:
2022年第5期
页码:
604-610
栏目:
医学信号处理与医学仪器
出版日期:
2022-05-27

文章信息/Info

Title:
Structural design and functional analysis of an apparatus for detection of circulating tumor cells
文章编号:
1005-202X（2022）05-0604-07
作者:
郭冰冰; 胡秀枋; 宋子玥; 黄馨雨; 沈亦纯; 邹任玲; 谷雪莲; 邸元帅; 郭宛星; 徐秀林
上海理工大学健康科学与工程学院， 上海 200093
Author(s):
GUO Bingbing;  HU Xiufang;  SONG Ziyue;  HUANG Xinyu;  SHEN Yichun;  ZOU Renling;  GU Xuelian;  DI Yuanshuai;  GUO Wanxing;  XU Xiulin
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
关键词:
微流控; 高通量; 循环肿瘤细胞; 早期筛查
Keywords:
Keywords: microfluidic high throughput circulating tumor cells early screening
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2022.05.014
文献标志码:
A
摘要:
为了满足患者对于高质量精准诊疗服务的需求，实现智能化、高通量、高检出率的无创早期筛查，研发一台基于微流控技术的循环肿瘤细胞（CTCs）检测仪。仪器提供高度集成和自动化微流控芯片，采用精准高效的荧光检测系统进行单细胞水平的CTCs检测，并通过检测系统进行程序化检测。仪器可对病人血液中的CTCs进行分离、富集和检测。将人体外周血液样本通过微流控分选芯片得到CTCs富集液，再通过微流控检测芯片和光路检测系统由上位机计数得到血液样本中CTCs的数量。使用裂解红细胞后的模拟血液样本进行了6次实验，在达到0.2 mL/h的最佳流速时， CTCs平均检出率能够达到75.9%以上，组内相关系数大于0.8，证明了该仪器具有较高的检出率和良好的可靠性，可为相关领域的技术突破提供良好的研究设施。
Abstract:
Abstract: In order to meet the needs of patients for high-quality and precision diagnosis and treatment services, and to achieve intelligent and high-throughput non-invasive early screening with high detection rate, an apparatus for the detection of circulating tumor cells (CTCs) is developed based on microfluidic technology. The apparatus provides highly-integrated and automated microfluidic chips, and uses precise and efficient fluorescence detection system for CTCs detection at the single cell level. The programmed detection is realized through the detection system. The apparatus can be used to separate, enrich and detect CTCs in patients blood. CTCs enrichment liquid is obtained from human peripheral blood samples by microfluidic sorting chip, and then the number of CTCs in blood samples is counted by upper computer through microfluidic detection chip and optical path detection system. The experiment on the simulated blood samples after red blood cell lysis is carried out for 6 times. At the optimal flow velocity of 0.2 mL/h, the CTCs detection rate is higher than 75.9% on average, and the intraclass correlation coefficient is greater than 0.8. It is proved that the apparatus has high detection rate and good reliability, and it can serve as a high-quality research facility for promoting technical breakthroughs in related fields.

相似文献/References:

[1]周星贝,丁涛,王树水.基于微透析与微流控的新型肝素实时监测方法[J].中国医学物理学杂志,2021,38(10):1289.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.019]
　ZHOU Xingbei,DING Tao,et al.Real-time monitoring of heparin concentration using microdialysis-microfluidics system[J].Chinese Journal of Medical Physics,2021,38(5):1289.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.019]

备注/Memo

备注/Memo:
【收稿日期】2021-11-19 【基金项目】上海市科委科技支撑计划（19441904500） 【作者简介】郭冰冰，硕士研究生，研究方向：医用体外循环设备及技术，E-mail: 2475834729@qq.com 【通信作者】徐秀林，教授，研究方向：康复设备及其关键技术、医用体外循环设备及技术、人工智能及大数据，E-mail: xxlin100@163.com
更新日期/Last Update: 2022-05-27