[1]陈祁青,赵继荣,蔡毅,等.基于静息态功能磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究[J].中国医学物理学杂志,2022,39(4):430-435.[doi:DOI:10.3969/j.issn.1005-202X.2022.04.007]
　CHEN Qiqing,ZHAO Jirong,CAI Yi,et al.Experimental study of chronic lower limb pain rats with lumbar disc herniation based on resting-state functional magnetic resonance morphology[J].Chinese Journal of Medical Physics,2022,39(4):430-435.[doi:DOI:10.3969/j.issn.1005-202X.2022.04.007]
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基于静息态功能磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
39卷
期数:
2022年第4期
页码:
430-435
栏目:
医学影像物理
出版日期:
2022-04-27

文章信息/Info

Title:
Experimental study of chronic lower limb pain rats with lumbar disc herniation based on resting-state functional magnetic resonance morphology
文章编号:
1005-202X（2022）04-0430-06
作者:
陈祁青1; 赵继荣1; 蔡毅2; 陈文1; 赵宁1; 马同1; 薛旭1; 郭培尧1; 张天龙2; 杨涛2; 李玮农2; 张立存2
1.甘肃省中医院脊柱骨二科， 甘肃 兰州730050； 2.甘肃中医药大学中医临床学院， 甘肃 兰州 730030
Author(s):
CHEN Qiqing1;  ZHAO Jirong1;  CAI Yi2;  CHEN Wen1;  ZHAO Ning1;  MA Tong1;  XUE Xu1;  GUO Peiyao1;  ZHANG Tianlong2;  YANG Tao2;  LI Weinong2;  ZHANG Licun2
1. Second Department of Spine, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China 2. Clinical College of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou 730030, China
关键词:
腰椎间盘突出症; 静息态功能磁共振; 分数低频振幅; 局部一致性
Keywords:
Keywords: lumbar disc herniation resting-state functional magnetic resonance imaging fractional amplitude of low frequency ?ctuation regional homogeneity
分类号:
R318；R681.5
DOI:
DOI:10.3969/j.issn.1005-202X.2022.04.007
文献标志码:
A
摘要:
【摘要】目的：基于静息态功能磁共振（RS-fMRI）技术和疼痛行为学技术，观察腰椎间盘突出症（LDH）慢性下肢痛模型大鼠脑结构及功能改变的特点，揭示LDH慢性下肢痛的脑机制。方法：选用6~8周龄健康的SD雄性大鼠24只，体质量（250±20） g，采用随机数字表法分为3组：正常组、假手术组、模型组，模型组采用自体髓核移植法建立LDH慢性下肢痛大鼠模型。各组分别于建模前0天、建模后第2、7、14、21、28天进行大体行为学观察、机械痛阈值测定、热痛阈值测定。每组随机选取4只大鼠分别于建模前0天、建模后第14、28天，利用Bruker公司7.0T核磁收集BOLD数据，采用局部一致性（ReHo）、分数低频振幅（fALFF）方法处理BOLD数据，对所得数据进行统计学分析。结果：（1）3组大鼠建模前机械缩足反射阈值（PWT）和热缩足反射潜伏期（TWL）比较差异无统计学意义（P>0.05）；假手术组建模后各时间点PWT和TWL与正常组比较，差异无统计学意义（P>0.05）；模型组大鼠建模后各时间点PWT和TWL与正常组、假手术组比较，差异有统计学意义（P<0.05）。（2）3组大鼠不同时间点脑结构区域比较存在交互作用，差异有统计学意义（P<0.05）。脑区图结果显示ReHo值存在显著差异的脑区有：左室周灰质、右纹状体；fALFF值存在显著差异的脑区有：右纹状体、左嗅球、双侧小脑分子层。结论：（1）大鼠采用自体髓核移植法进行LDH建模后，机械痛阈值和热痛阈值明显降低，出现痛觉过敏反应，提示LDH慢性下肢痛大鼠建模成功；（2）LDH慢性下肢痛模型大鼠静息态下某些脑区功能活动有明显改变，可能与慢性疼痛的发生和维持有关。
Abstract:
Abstract: Objective To reveal the brain mechanism of chronic lower limb pain due to lumbar disc herniation (LDH) by observing the changes of brain structures and functions in rat model of LDH chronic lower limb pain using resting-state functional magnetic resonance imaging (RS-fMRI) and pain behavior assessment. Methods A total of 24 healthy SD male rats aged 6-8 weeks with a body weight of (250±20) g were randomly divided into 3 groups, namely normal group, sham operation group and model group. The LDH chronic lower limb pain rat model in model group was established by autologous nucleus pulposus transplantation. Gross behavioral observation, paw withdrawal threshold (PWT) and thermal withdrawal latency (TWL) measurements were carried out in all groups before modeling and on the 2nd, 7th, 14th, 21st, and 28th days after modeling. Before modeling and on the 14th and 28th day after modeling, 4 rats were randomly selected from each group to collect BOLD data using Bruker 7.0T nuclear magnetic field. The obtained BOLD data were processed by regional homogeneity (ReHo) and fractional amplitude of low-frequency fluctuation (fALFF) for statistical analysis. Results (1) There was no statistically significant difference in PWT and TWL among 3 groups of rats before modeling (P>0.05). At each time point after modeling, the differences in PWT and TWL between sham operation group and normal group were trivial (P>0.05), but the PWT and TWL in model group were different from those of normal group and sham operation group, with statistical significance (P<0.05). (2) The 3 groups of rats had an interactive effect on the brain structure at different time points, and the difference was statistically significant (P<0.05). The brain map results show that the ReHo value changed obviously in left periventricular grey matter and right striatum, and that the brain regions with significant differences in fALFF value included right striatum, left olfactory bulb and molecular layer of the cerebellum. Conclusion (1) After rats were modeled with LDH by autologous nucleus pulposus transplantation, PWT and TWL were significantly reduced, and hyperalgesia occurred, suggesting that LDH chronic lower limb pain rat model is successfully established. (2) The functional activities of some brain regions in LDH chronic lower limb pain rat model at resting state change significantly, which may be related to the occurrence and maintenance of chronic pain.

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备注/Memo

备注/Memo:
【收稿日期】2021-07-22 【基金项目】国家自然科学基金（81760877）；中医药循证能力建设项目（2019XZZX-GK005）；甘肃省中医药管理局科研课题（GZK-2018-35）；甘肃省自然科学基金（17JR5RA051）；兰州市创新人才项目（2018-RC-99）；甘肃省科技厅重点研发项目（20YF3FA016） 【作者简介】陈祁青，副主任医师，主要从事脊柱疾病的中西医结合临床及基础研究，E-mail: 13282412616@163.com 【通信作者】赵继荣，主任医师，博士生导师，主要从事中医药防治脊柱相关疾病研究，E-mail: 360529338@qq.com
更新日期/Last Update: 2022-04-27