[1]范义湘,易紫薇,胡煜麟,等.NIS基因转染对肝细胞癌NIS蛋白表达及摄碘功能的影响[J].中国医学物理学杂志,2021,38(10):1219-1222.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.007]
　FAN Yixiang,YI Ziwei,HU Yulin,et al.Effects of NIS gene transfection on NIS protein expression and iodine uptake in hepatocellular carcinoma[J].Chinese Journal of Medical Physics,2021,38(10):1219-1222.[doi:DOI:10.3969/j.issn.1005-202X.2021.10.007]
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NIS基因转染对肝细胞癌NIS蛋白表达及摄碘功能的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第10期
页码:
1219-1222
栏目:
医学放射物理
出版日期:
2021-10-27

文章信息/Info

Title:
Effects of NIS gene transfection on NIS protein expression and iodine uptake in hepatocellular carcinoma
文章编号:
1005-202X（2021）10-1219-04
作者:
范义湘1; 易紫薇1; 胡煜麟1; 张宏嘉1; 林美珍1; 陈洁芳1; 肖汉2
1.南方医科大学第五附属医院核医学科， 广东 广州 510900； 2.广东省第二人民医院核医学科， 广东 广州 510317
Author(s):
FAN Yixiang1;  YI Ziwei1;  HU Yulin1;  ZHANG Hongjia1;  LIN Meizhen1;  CHEN Jiefang1;  XIAO Han2
1. Department of Nuclear Medicine, the Fifth Affiliated Hospital of Southern Medical University, Guangzhou 510900, China 2. Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China
关键词:
肝细胞癌; 钠碘转运体; 放射性碘治疗; NIS基因; 摄碘功能; 细胞凋亡
Keywords:
Keywords: hepatocellular carcinoma sodium/iodine symporter radioactive iodine therapy NIS gene iodine uptake apoptosis
分类号:
R34；R817.1
DOI:
DOI:10.3969/j.issn.1005-202X.2021.10.007
文献标志码:
A
摘要:
目的：探讨肝细胞癌细胞核素靶向治疗方法。方法：提取NIS基因片段并构建重组质粒pcDNA3/NIS，将重组质粒导入肝细胞癌HepG2细胞。转染后24 h利用Western-Blot法检测HepG2细胞NIS蛋白表达，采用125I结合试验评估转染后细胞的摄碘率，采用DAPI染色法评估HepG2细胞摄125I后的凋亡情况。实验组与对照组之间NIS蛋白表达、摄碘率以及细胞凋亡率比较采用t检验。结果：蛋白电泳表明经NIS基因转染后，实验组HepG2细胞已表达NIS蛋白，表达强度显著高于对照组（t=2.693, P<0.05）。实验组HepG2细胞摄碘率B/T%平均为（18.4±5.8）%，显著高于对照组（t=36.842, P<0.05）。结合125I后24 h，实验组凋亡细胞数多于对照组，平均凋亡率为（19.2±5.3）%，显著高于对照组（t=3.086, P<0.05）。结论：转染外源性NIS基因可上调肝细胞癌HepG2细胞NIS蛋白表达，使其具备摄碘功能，加快细胞凋亡，为放射性碘靶向治疗提供实验依据。
Abstract:
Abstract: Objective To explore the method of targeted radionuclide therapy for hepatocellular carcinoma. Methods The NIS gene fragment was extracted, and the recombinant plasmid pCDNA3/NIS was constructed. The recombinant plasmid was introduced into hepatocellular carcinoma HepG2 cells. The NIS protein expression in HepG2 cells at 24 h after transfection was detected by Western-Blot assay and the iodine uptake rate of the transfected cells was evaluated by 125I binding assay and the apoptosis of HepG2 cells after 125I uptake was evaluated by DAPI staining. NIS protein expression, iodine uptake rate and apoptosis rate were compared between experimental group and control group using t test. Results Protein electrophoresis showed that after NIS gene transfection, NIS protein was expressed in HepG2 cells in experimental group, and the expression intensity was significantly higher than that in control group (t=2.693,P<0.05). The iodine uptake rate (B/T%) of HepG2 cells in experimental group was (18.4±5.8)%, significantly higher than that of control group (t=36.842, P<0.05). At 24 h after 125I uptake, the number of apoptotic cells in experimental group was larger than that in control group, and the average apoptosis rate in experimental group was (19.2±5.3)%, significantly higher than that in control group (t=3.086, P<0.05). Conclusion The transfection of exogenous NIS gene can up-regulate NIS protein expression in hepatocellular carcinoma HepG2 cells, enable the cells to have the function of iodine uptake, and accelerate cell apoptosis. The study provide an experimental basis for targeted radionuclide therapy.

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备注/Memo

备注/Memo:
【收稿日期】2021-05-18 【作者简介】范义湘，主任医师，硕士生导师，研究方向：甲状腺癌的核医学诊断与治疗，E-mail: 13802990971@163.com
更新日期/Last Update: 2021-10-28