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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:

37

期数:

2020年第9期

页码:

1193-1197

栏目:

医学人工智能

出版日期:

2020-09-25

文章信息/Info

Title:

Effect of electret combined with 5-fluorouracil on TGF-β/MMPs pathway during scar formation

文章编号:

1005-202X（2020）09-1193-05

作者:

郭鑫1; 涂晔2; 苑旺3; 梁媛媛1; 江键1; 崔黎丽4

1. 海军军医大学卫生勤务学系数理教研室，上海200433；2. 上海东方医院，上海200120；3. 北部战区总医院，辽宁沈阳110000； 4. 海军军医大学药学院无机化学教研室，上海200433

Author(s):

GUO Xin1;  TU Ye2;  YUAN Wang3;  LIANG Yuanyuan1;  JIANG Jian1;  CUI Lili4

1. Department of Physics and Mathematics, Faculty of Health Service, the Second Military Medical University, Shanghai 200433, China 2. Shanghai East Hospital, Shanghai 200120, China 3. General Hospital of Northern Theater, Shenyang 110000, China 4. Department of Inorganic Chemistry, School of Pharmacy, the Second Military Medical University, Shanghai 200433, China

关键词:

增生性瘢痕; 5-氟尿嘧啶; 驻极体; 转化生长因子-β; 基质金属蛋白酶

Keywords:

hypertrophic scar 5-fluorouracil electret TGF-βMMPs

分类号:

R318；R34

DOI:

10.3969/j.issn.1005-202X.2020.09.021

文献标志码:

A

摘要:

目的：研究驻极体联合5-氟尿嘧啶（5-Fu）对瘢痕生长的抑制作用及其相关的作用机制。方法：将驻极体贴剂、5- Fu 贴剂、驻极体联合5-Fu 贴剂分别作用于大鼠创伤创面愈合的瘢痕形成过程，通过对创面组织瘢痕增生指数、显微结构 以及组织中TGF-β、MMP1、TIMP1 和MMP2 含量情况的检测，研究驻极体以及驻极体与5-Fu 联合对瘢痕形成的抑制作 用及作用机制。结果：（1）驻极体、5-Fu 以及驻极体联合5-Fu 贴剂均能有效抑制瘢痕的形成和生长。三者分别作用创面 4 周后，瘢痕增生指数依次是对照组的85.8%、85.1%和64.9%（P<0.05）。（2）驻极体、5-Fu 以及驻极体与5-Fu 联合均能影 响瘢痕形成中的TGF-β/MMPs 通路。表现为三者均能抑制TGF-β 的表达，上调MMP1 的表达，增加MMP1/TIMP1 比例， 下调MMP2 表达。并且作用效果为驻极体联合5-Fu>5-Fu>驻极体。结论：与5-Fu 一样，驻极体也能抑制瘢痕组织的生 长，且两者具有协同作用；它们的作用机制与瘢痕组织内TGF-β、MMP1、TIMP1 和MMP2 的表达有关；驻极体与5-Fu 联 用后，在相同治疗效果的基础上，可减少5-Fu 的剂量，降低它的毒副作用。

Abstract:

Objective To investigate the inhibiting effect of electret combined with 5-fluorouracil (5-Fu) on scar formation and discuss the related mechanisms. Methods Different patches, including electret, 5-Fu, and electret combined with 5-Fu, were separately applied to the wound in mice, participating in the formation of hypertrophic scar. The scar hyperplasia index and microstructure of wound tissues and the expressions of TGF-β,MMP1, TIMP1 andMMP2 in tissues were detected for studying the inhibiting effect of electret combined with 5-Fu on scar formation and discussing the related mechanisms . Results (1) The formation and growth of scar were effectively inhibited by electret, 5-Fu, and electret combined with 5-Fu, separately. After 4 weeks of treatments by different patches, the scar hyperplasia index was decreased to 85.8%, 85.1% and 64.9% of that in control group, respectively (P<0.05). (2) All the treatments (electret, 5-Fu, and electret combined with 5-Fu) had effects on TGF-β/MMPs pathway during scar formation. In treatment groups, TGF-β expression was inhibited and MMP1 expression was up-regulated and MMP1/TIMP1 ratio was increased whereas MMP2 was down-regulated. The effect of electret combined 5-Fu group was better than that of 5-Fu, followed by electret. Conclusion Like 5-Fu, electret can inhibit the growth of scar, and they have a synergistic effect. The mechanism may be related to the expressions of TGF-β, MMP1, TIMP1 and MMP2. The electret combined with 5-Fu is expected to improve the bioavailability of 5-Fu (reduce 5-Fu dose and decrease its toxic effects) on the basis of achieving the same therapeutic effect.

备注/Memo

备注/Memo:

【收稿日期】2020-04-25 【基金项目】国家自然科学基金（51477175）；上海市卫计委青年科学基金（20164Y0051） 【作者简介】郭鑫，硕士，讲师，主要研究方向：电磁场的生物效应，E-mail:501179450@qq.com；涂晔，博士，主要研究方向：静电场的透皮给药研究， E-mail: dan16065@163.com 【通信作者】江键，教授，主要研究方向：驻极体生物效应在医学中的应用和驻极体缓控释透皮给药系统，E-

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更新日期/Last Update: 2020-09-25