[1]霍博,丁元钧,蔡婧,等.低强度脉冲电磁场加速2型糖尿病表皮软组织创伤愈合实验[J].中国医学物理学杂志,2020,37(7):912-917.[doi:DOI:10.3969/j.issn.1005-202X.2020.07.022]
　HUO Bo,DING Yuanjun,CAI Jing,et al.Low-intensity pulsed electromagnetic fields accelerates skin wound healing in type 2 diabetic mice: an experimental research[J].Chinese Journal of Medical Physics,2020,37(7):912-917.[doi:DOI:10.3969/j.issn.1005-202X.2020.07.022]
点击复制

低强度脉冲电磁场加速2型糖尿病表皮软组织创伤愈合实验()
分享到：

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第7期
页码:
912-917
栏目:
医学生物物理
出版日期:
2020-07-25

文章信息/Info

Title:
Low-intensity pulsed electromagnetic fields accelerates skin wound healing in type 2 diabetic mice: an experimental research
文章编号:
1005-202X（2020）07-0912-06
作者:
霍博1; 丁元钧2; 蔡婧2; 张美霞3
1.空军军医大学第一附属医院呼吸内科， 陕西 西安710032； 2.空军军医大学军事生物医学工程学系， 陕西 西安710032； 3.空军军医大学第一附属医院护理部， 陕西 西安710032
Author(s):
HUO Bo1;  DING Yuanjun2;  CAI Jing2;  ZHANG Meixia3
1. Department of Respiratory Medicine, the First Affiliated Hospital of Air Force Medical University of PLA, Xian 710032, China 2. Department of Military Biomedical Engineering, Air Force Medical University of PLA, Xian 710032, China 3. Nursing Department, the First Affiliated Hospital of Air Force Medical University of PLA, Xian 710032, China
关键词:
2型糖尿病; 软组织创伤; 低强度脉冲电磁场; 炎性因子; 抗张强度
Keywords:
Keywords: type 2 diabetes mellitus soft tissue wound low-intensity pulsed electromagnetic field inflammatory cytokines tensile strength
分类号:
R318；Q64
DOI:
DOI:10.3969/j.issn.1005-202X.2020.07.022
文献标志码:
A
摘要:
目的：研究低强度的脉冲电磁场（PEMF）对于2型糖尿病软组织创伤愈合的治疗效果。方法：32只3月龄雄性2型糖尿病db/db小鼠及16只同背景的野生对照小鼠用于本研究，分为Wild type野生型小鼠组（Wild type）、db/db小鼠组（db/db）及db/db施加以PEMF刺激组（db/db+PEMF），每组16只。分别于创伤模型构建的第5、12、19天处死每组的4只小鼠。全部小鼠均构建背部表皮创伤模型，db/db+PEMF组小鼠施以全身PEMF刺激，每天刺激2 h。分别通过伤口闭合率、伤口组织抗张强度、伤口组织病理和ELISA检测分析评估其作用效果。结果：PEMF刺激在术后的第5、12、19天均显著提升了db/db小鼠伤口的愈合速率（P<0.05），降低了db/db小鼠的伤口总愈合时间（P<0.05），在术后的第5、12、19天均显著提升了伤口组织的抗张强度（P<0.05），降低了伤口组织的炎性细胞数量且显著降低了炎性因子IL-1β和TNF-α的表达（P<0.05），并上调了VEGF和IGF-1的表达（P<0.05）。结论：低强度PEMF能够显著加速2型糖尿病db/db小鼠的软组织修复进程，这一效应可能与PEMF的抗炎性反应及促胶原沉积和血管再生相关。
Abstract:
Abstract: Objective To identify the therapeutic effects of low-intensity pulsed electromagnetic fields (PEMF) on the soft tissue wound healing in type 2 diabetic mice. Methods Thirty-two 3-month-old male type 2 diabetic db/db mice and 16 wild type control mice with the same background were assigned to db/db group (db/db) and db/db treated with PEMF group (db/db+PEMF) and wild type group (Wild type), with 16 mice in each group. Four mice in each group were sacrificed on day 5, 12 and 19 after the wound model establishment. The induction of dorsal wound model was performed on all mice. The mice in db/db+PEMF group were subjected to whole-body PEMF stimulation for 2 h per day. The therapeutic effects were evaluated by wound healing rate, wound tensile strength, wound tissue histopathology and ELISA assay. Results PEMF stimulation significantly increased the wound healing rate in db/db mice on the 5th, 12nd and 19th day postoperatively (P<0.05), and also decreased the overall wound healing period in db/db mice (P<0.05). Moreover, PEMF simulation increased wound tensile strength on the 5th, 12nd and 19th day postoperatively (P<0.05), and also decreased the number of inflammatory cells in wound tissues, suppressed the expressions of inflammatory cytokines IL-1β and TNF-α (P<0.05), increased the expressions of VEGF and IGF-1 (P<0.05). Conclusion Low-intensity PEMF stimulation is capable of significantly accelerating soft tissue healing in type 2 diabetic db/db mice, which may be related to its anti-inflammatory response and promotion of collagen deposition and vascular regeneration.

相似文献/References:

[1]王磊,肖洪彬,牛雯颖,等.流式细胞术对2型糖尿病大鼠红细胞凋亡的研究[J].中国医学物理学杂志,2015,32(05):660.[doi:doi:10.3969/j.issn.1005-202X.2015.05.010]
[2]刘姗姗,王晓艳,王鹏程,等.伴有视网膜病变的2型糖尿病患者大脑静息态功能磁共振成像低频振幅研究[J].中国医学物理学杂志,2016,33(11):1149.[doi:10.3969/j.issn.1005-202X.2016.11.013]
　[J].Chinese Journal of Medical Physics,2016,33(7):1149.[doi:10.3969/j.issn.1005-202X.2016.11.013]
[3]魏统辉,刘新凤,崔栋,等. 2型糖尿病患者大脑灰质密度改变及其与认知受损的相关性研究[J].中国医学物理学杂志,2018,35(3):364.[doi:DOI:10.3969/j.issn.1005-202X.2018.03.021]
　WEI Tonghui,LIU Xinfeng,CUI Dong,et al. Gray matter density alteration and its correlation with cognitive impairment in patients with type 2 diabetes mellitus[J].Chinese Journal of Medical Physics,2018,35(7):364.[doi:DOI:10.3969/j.issn.1005-202X.2018.03.021]
[4]王伟茜,刘新凤,曹卫芳,等.2型糖尿病磁共振成像静息态低频振幅变化及其与认知受损的相关性[J].中国医学物理学杂志,2018,35(5):543.[doi:DOI:10.3969/j.issn.1005-202X.2018.05.009]
　WANG Weiqian,LIU Xinfeng,CAO Weifang,et al.Altered fractional amplitude of low-frequency fluctuation of fMRI signals and its correlation with cognitive impairment in type 2 diabetes mellitus patients[J].Chinese Journal of Medical Physics,2018,35(7):543.[doi:DOI:10.3969/j.issn.1005-202X.2018.05.009]
[5]李煜瑶,李静,焦青,等. 2型糖尿病患者功能磁共振成像的局部脑区活动时空四维一致性[J].中国医学物理学杂志,2019,36(10):1162.[doi:DOI:10.3969/j.issn.1005-202X.2019.10.009]
　LI Yuyao,LI Jing,JIAO Qing,et al. Four-dimensional (spatio-temporal) consistency of local neural activity in type 2 diabetes patient on fMRI[J].Chinese Journal of Medical Physics,2019,36(7):1162.[doi:DOI:10.3969/j.issn.1005-202X.2019.10.009]

备注/Memo

备注/Memo:
【收稿日期】2020-02-27 【基金项目】陕西省自然科学基金（2018JQ8056） 【作者简介】霍博，护师，从事护理相关的临床和科研工作，E-mail: 1217912907@qq.com 【通信作者】张美霞，主任护师，从事护理相关的临床和科研工作，E-mail: 2556008698@qq.com
更新日期/Last Update: 2020-07-28