[1]张广伟,梁卓文,杨治.Ca2+在电磁场调控成骨细胞增殖与分化中的作用[J].中国医学物理学杂志,2024,41(1):95-100.[doi:DOI:10.3969/j.issn.1005-202X.2024.01.014]
　ZHANG Guangwei,LIANG Zhuowen,YANG Zhi.Role of Ca2+ in electromagnetic field regulation on osteoblast proliferation and differentiation[J].Chinese Journal of Medical Physics,2024,41(1):95-100.[doi:DOI:10.3969/j.issn.1005-202X.2024.01.014]
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Ca2+在电磁场调控成骨细胞增殖与分化中的作用()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
41卷
期数:
2024年第1期
页码:
95-100
栏目:
医学生物物理
出版日期:
2024-01-23

文章信息/Info

Title:
Role of Ca2+ in electromagnetic field regulation on osteoblast proliferation and differentiation
文章编号:
1005-202X（2024）01-0095-06
作者:
张广伟1; 梁卓文2; 杨治3
1.陕西中医药大学第二临床医学院， 陕西 咸阳 712046； 2.空军军医大学西京医院骨科， 陕西 西安 710032； 3.红会医院关节病医院关节翻修病区， 陕西 西安 710054
Author(s):
ZHANG Guangwei1;  LIANG Zhuowen2;  YANG Zhi3
1. The Second Clinical Medical College of Shaanxi University of Chinese Medicine, Xianyang 712046, China 2. Department of Orthopedics, Xijing Hospital, Air Force Medical University, Xian 710032, China 3. Joint Revision Unit, Arthritis Hospital, Honghui Hospital, Xian 710054, China
关键词:
电磁场; 成骨细胞; 钙离子; 细胞增值; 细胞分化
Keywords:
Keywords: electromagnetic field osteoblast calcium ion cell proliferation cell differentiation
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2024.01.014
文献标志码:
A
摘要:
目的：从定性和定量的角度探究电磁场（EMF）对成骨细胞内Ca2+变化的影响，并试图探明Ca2+在电磁场调控成骨细胞增殖与分化中的作用。方法：搭建频率为38.7 Hz，强度为1.5 mT的正弦EMF发生平台，将MC3T3-E1成骨细胞随机分为对照组和实验（EMF）组。EMF组施加EMF干预，每天干预8 h。利用CCK8检测成骨细胞增殖，ALP染色检测成骨细胞分化，Ca2+荧光探针和流式细胞仪检测成骨细胞内Ca2+浓度。结果：CCK8细胞增殖结果显示，EMF干预48、72、96及120 h，可显著促进成骨细胞增殖。ALP染色结果显示EMF干预14 d后，EMF组ALP表达显著高于对照组。Ca2+荧光探针及流式细胞仪检测Ca2+浓度结果显示EMF干预可促进成骨细胞内Ca2+的增多。结论：EMF上调成骨细胞内Ca2+信号可能与EMF促成骨细胞增殖和分化密切相关，但是哪些与Ca2+相关的生物信号通路参与了EMF促成骨细胞增殖和分化效应，有待进一步研究。
Abstract:
Abstract: Objective To explore the effects of electromagnetic field (EMF) on the change of Ca2+ in osteolbast from the qualitative and quantitative perspectives, and try to identify the role of Ca2+ in EMF regulation on osteoblast proliferation and differentiation. Methods A platform was established for generating sine EMF with a frequency of 38.7 Hz and a strength of 1.5 mT. The MC3T3-E1 osteoblasts were randomly divided into control group and experimental group (EMF intervention for 8 h per day). CCK8 was used to detect osteoblast proliferation, ALP staining to detect osteoblast differentiation, and Ca2+ fluorescence probes and flow cytometer to detect the Ca2+ concentration in osteoblasts. Results CCK8 result showed that EMF intervention for 48, 72, 96 and 120 h could significantly promote osteoblast proliferation. After 14 days of EMF intervention, the positive expression of ALP was significantly higher in EMF group than in control group. Ca2+ fluorescent staining and flow cytometry results revealed that EMF intervention could increase the Ca2+ in osteoblasts. Conclusion The EMF-induced upregulation of Ca2+ signal in osteoblasts may be closely related to the promotions of osteoblast proliferation and differentiation by EMF, but which Ca2+-related biosignaling pathways are involved in the EMF promoting osteoblast proliferation and differentiation remains to be further investigated.

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备注/Memo

备注/Memo:
【收稿日期】2023-09-08 【基金项目】国家自然科学基金（51907197） 【作者简介】张广伟，硕士研究生，主治医师，研究方向：关节修复，E-mail: zgw803@qq.com 【通信作者】杨治，博士，主任医师，研究方向：股骨头坏死，E-mail: 382913402@qq.com
更新日期/Last Update: 2024-01-23