[1]王婷婷,黄巧红,林鑫鑫,等.不同单次剂量中波紫外线照射对大鼠角膜的影响[J].中国医学物理学杂志,2023,40(4):473-479.[doi:DOI:10.3969/j.issn.1005-202X.2023.04.013]
　WANG Tingting,HUANG Qiaohong,LIN Xinxin,et al.Effects of different single doses of wltraviolet B on cornea of rats[J].Chinese Journal of Medical Physics,2023,40(4):473-479.[doi:DOI:10.3969/j.issn.1005-202X.2023.04.013]
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不同单次剂量中波紫外线照射对大鼠角膜的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
40卷
期数:
2023年第4期
页码:
473-479
栏目:
医学生物物理
出版日期:
2023-04-25

文章信息/Info

Title:
Effects of different single doses of wltraviolet B on cornea of rats
文章编号:
1005-202X（2023）04-0473-07
作者:
王婷婷; 黄巧红; 林鑫鑫; 杨倩; 朱镜谕; 黄焱
福建医科大学医学技术与工程学院眼视光学系， 福建 福州 350004
Author(s):
WANG Tingting;  HUANG Qiaohong;  LIN Xinxin;  YANG Qian;  ZHU Jingyu;  HUANG Yan
Department of Ophthalmology and Optometry, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China
关键词:
紫外线; 照射剂量; 角膜; 醛脱氢酶
Keywords:
Keywords: ultraviolet irradiation dose cornea aldehyde dehydrogenase
分类号:
R772.2
DOI:
DOI:10.3969/j.issn.1005-202X.2023.04.013
文献标志码:
A
摘要:
目的：探究在中波紫外线（UVB）照射总剂量恒定的情况下，不同单次剂量照射对大鼠角膜结构和功能的影响。方法：40只健康SD大鼠随机分为4组（每组各10只），对照组自然光环境下饲养，第42天取材，实验组每日UVB的照射强度为146 μW/cm?每组累计照射总剂量一致，分为单次低剂量组、中剂量组、高剂量组，分别每日照射12、24、48 min，于连续照射第84、42、21天取材，行裂隙灯显微镜检查、角膜荧光素钠染色、泪膜破裂时间（BUT）测定，TUNEL检测角膜细胞凋亡，ELISA检测醛脱氢酶（ALDH）的活性及表达量。结果：对照组大鼠角膜透明，实验组随单次照射剂量的升高，大鼠角膜混浊程度加重。对照组大鼠角膜荧光素钠染色评分为0分，实验组大鼠分别为（4.30±1.42）分、（8.90±1.73）分、（11.10±1.97）分。实验组大鼠BUT值均较对照组下降（P<0.05），且随单次照射剂量的升高而降低。实验组大鼠角膜细胞凋亡指数均较对照组升高（P<0.05），且随单次照射剂量的升高而升高。单次低剂量组与对照组大鼠角膜厚度比较，差异没有统计学意义（P>0.05），单次中剂量组、单次高剂量组大鼠角膜厚度均比对照组降低（P<0.05）。实验组大鼠角膜ALDH活性及表达量均较对照组降低（P<0.05），且随单次照射剂量的升高而降低更加显著。结论：UVB照射总剂量恒定，单次高剂量UVB照射导致大鼠角膜混浊、角膜变薄且可能诱发圆锥角膜。这些损伤与ALDH活性及表达量下降导致的抗氧化能力下降有关，提示可以通过提高ALDH活性保护角膜减少紫外线损伤。
Abstract:
Abstract: Objective To explore the effects of different single doses of ultraviolet B (UVB) on the structure and function of rat cornea under the condition that the total dose of UVB irradiation is constant. Methods Forty healthy SD rats were randomly divided into 4 groups (10 rats in each group). The rats in control group were raised under natural light, and the samples were taken on day 42. The daily UVB irradiation intensity in experimental groups remained at 146 μW/cm? and each group had the same cumulative irradiation dose. The rats in single low-, medium- and high-dose groups were exposed to daily UVB irradiation for 12, 24 and 48 min, respectively. On the 84th, 42nd and 21st days of continuous irradiation, the samples were collected for slit-lamp examination, corneal fluorescein sodium staining, and tear film break-up time (BUT) test. TUNEL was adopted to detect corneal cell apoptosis, and ELISA was used to detect the activity and expression of aldehyde dehydrogenase (ALDH). Results The rats in control group maintain corneal transparence, while in experimental groups, with the increase of the single irradiation dose, the degree of corneal opacity was aggravated. The corneal fluorescein sodium staining scores in control group and experimental groups were 0, (4.30±1.42), (8.90±1.73), and (11.10±1.97), respectively. Compared with control group, experimental groups had shorter BUT (P<0.05) which was shortened with the increase of the single irradiation dose, and higher apoptosis index (P<0.05) which was increased with the increase of single irradiation dose. There was no significant difference in corneal thickness between single low-dose group and control group (P>0.05), but the corneal thickness were thinner in single medium- and high-dose groups than in control group (P<0.05). The activity and expression of corneal ALDH in experimental groups were lower than those in control group (P<0.05), and the decrease was more significant with the increase of single irradiation dose. Conclusion When the total dose of UVB irradiation is constant, single high dose of UVB irradiation results in corneal opacity and corneal thinning in rats and may induce keratoconus. These damages are related to the decreased antioxidant capacity caused by the decreased activity and expression of ALDH, which suggests that it can protect the cornea and reduce UV damage by increasing the activity of ALDH.

相似文献/References:

[1]袁惠萍,何婉玲,梁月娥,等. 空气净化与紫外线在介入手术室空气消毒效果比较[J].中国医学物理学杂志,2019,36(8):981.[doi:DOI:10.3969/j.issn.1005-202X.2019.08.022]
　YUAN Huiping,HE Wanling,LIANG Yuee,et al. Comparison between air purification equipment and ultraviolet radiation for the air disinfection of interventional room[J].Chinese Journal of Medical Physics,2019,36(4):981.[doi:DOI:10.3969/j.issn.1005-202X.2019.08.022]

备注/Memo

备注/Memo:
【收稿日期】2022-10-18 【基金项目】福建省自然科学基金（2020J01652） 【作者简介】王婷婷，硕士研究生，研究方向：眼视光学，E-mail: 502193390@qq.com 【通信作者】黄焱，博士，教授，研究方向：眼视光学、眼科学，E-mail: fjhyan2005@139.com
更新日期/Last Update: 2023-04-25