[1]柏中喜,方兴刚,马龙祥,等.脉冲电磁场通过Wntβ-catenin信号通路改善膝骨关节炎大鼠炎症反应的机制[J].中国医学物理学杂志,2022,39(5):617-622.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.016]
　BAI Zhongxi,FANG Xinggang,MA Longxiang,et al.Mechanism of pulsed electromagnetic field in inhibiting inflammatory response in knee osteoarthritis rats through Wntβ-catenin signaling pathway[J].Chinese Journal of Medical Physics,2022,39(5):617-622.[doi:DOI:10.3969/j.issn.1005-202X.2022.05.016]
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脉冲电磁场通过Wntβ-catenin信号通路改善膝骨关节炎大鼠炎症反应的机制()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
39卷
期数:
2022年第5期
页码:
617-622
栏目:
医学生物物理
出版日期:
2022-05-27

文章信息/Info

Title:
Mechanism of pulsed electromagnetic field in inhibiting inflammatory response in knee osteoarthritis rats through Wntβ-catenin signaling pathway
文章编号:
1005-202X（2022）05-0617-06
作者:
柏中喜; 方兴刚; 马龙祥; 贺昱霖
十堰市太和医院（湖北医药学院附属医院）康复中心水疗理疗科， 湖北 十堰 442000
Author(s):
BAI Zhongxi;  FANG Xinggang;  MA Longxiang;  HE Yulin
Department of Hydrotherapy and Physiotherapy, Rehabilitation Center, Shiyan Taihe Hospital (Affiliated Hospital of Hubei University of Medicine), Shiyan 442000, China
关键词:
膝骨关节炎; 脉冲电磁场; Wntβ-catenin信号; 炎症反应
Keywords:
Keywords: knee osteoarthritis pulsed electromagnetic field Wntβ-catenin signal inflammatory response
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2022.05.016
文献标志码:
A
摘要:
目的：探讨脉冲电磁场通过Wntβ-catenin信号转导机制改善膝骨关节炎大鼠炎症反应的机制。方法：选取90只SD大鼠，均分为正常组、模型组和脉冲电磁场组，除正常组，其余各组均构建膝骨关节炎模型。测定各组大鼠局部皮温、膝关节周径和Lequesne MG评分；采取甲苯胺蓝染色进行Mankins评分；Western Blot检测软骨细胞凋亡调控蛋白Caspase-3和Caspase-8水平；ELISA法测定滑膜IL-1β、MMP-13、TNF-α表达；并检测Wnt和β-catenin mRNA和蛋白表达差异；检测大鼠线粒体膜电位。结果：与正常组相比，模型组大鼠膝关节局部皮温、膝关节周径、Lequesne MG评分、Mankins评分、Wntβ-catenin表达以及滑膜IL-1β、MMP-13、TNF-α水平均上调（P<0.05）；与模型组比较，脉冲电磁场组膝关节周径、Lequesne MG评分，Mankins评分、Wntβ-catenin表达以及滑膜IL-1β、MMP-13、TNF-α水平均下调（P<0.05）。相较于正常组，其余2组的关节软骨线粒体膜电位均有所下调。与模型组相比，脉冲电磁场组线粒体膜电位有所上调，脉冲电磁场组优于模型组。结论：脉冲电磁场能抑制膝骨关节炎模型大鼠炎症反应，有效修复软骨损伤，降低炎症因子表达，抑制Wntβ-catenin信号转导。
Abstract:
Abstract: Objective To explore the mechanism of pulsed electromagnetic field in reducing inflammatory response in knee osteoarthritis rats through the signal transduction mechanism of Wntβ-catenin. Methods A total of 90 SD rats were selected and divided into normal group, model group and pulsed electromagnetic field group, with 30 rats each. Except for normal group, the rat model of knee osteoarthritis was constructed in the other two groups. The local skin temperature, knee circumference and Lequesne MG score were measured in each group. Toluidine blue staining was used for Mankins scoring, Western Blot for obtaining the expression levels of apoptosis-regulating proteins Caspase-3 and Caspase-8, and ELISA for determining the expression levels of IL-1β, MMP-13 and TNF-α in synovial membrane. Moreover, the differences in mRNA and protein expressions of Wnt and β-catenin were analyzed, and the mitochondrial membrane potential was also measured. Results Compared with those in normal group, the local skin temperature, knee circumference, Lequesne MG score, Mankins score, expression levels of Wntβ-catenin, synovial IL-1β, MMP-13 and TNF-α in model group were significantly up-regulated (P<0.05). The knee circumference, Lequesne MG score, Mankins score, expression levels of Wntβ-catenin, synovial IL-1β, MMP-13 and TNF-α in pulsed electromagnetic field group were all significantly down-regulated as compared with model group (P<0.05). Compared with that in normal group, the mitochondrial membrane potential of articular cartilage in the other two groups was decreased, and the mitochondrial membrane potential of pulsed electromagnetic field group was lower than that of model group. Conclusion Pulse electromagnetic field can reduce the inflammatory response in knee osteoarthritis model rats, repair cartilage injury effectively, down-regulate the expression of inflammatory factors and inhibit Wntβ-catenin signal transduction.

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备注/Memo

备注/Memo:
【收稿日期】2021-12-15 【基金项目】湖北省卫生厅中医药科研重点项目（2012Z—Y48）；十堰市引导性科研项目（19Y27） 【作者简介】柏中喜，主管技师，研究方向：肌骨疼痛体外冲击波治疗，E-mail: baizhx1980@163.com 【通信作者】方兴刚，硕士，主治医师，研究方向：疼痛康复治疗，E-mail: 13593729928@163.com
更新日期/Last Update: 2022-05-27