[1]赵继荣,薛旭,袁毓喆,等.基于体素的磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究[J].中国医学物理学杂志,2021,38(7):820-825.[doi:DOI:10.3969/j.issn.1005-202X.2021.07.006]
　ZHAO Jirong,XUE Xu,YUAN Yuzhe,et al.Chronic lower extremity pain due to lumbar disc herniation: an experimental study in rats using voxel-based magnetic resonance morphometry[J].Chinese Journal of Medical Physics,2021,38(7):820-825.[doi:DOI:10.3969/j.issn.1005-202X.2021.07.006]
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基于体素的磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第7期
页码:
820-825
栏目:
医学影像物理
出版日期:
2021-07-26

文章信息/Info

Title:
Chronic lower extremity pain due to lumbar disc herniation: an experimental study in rats using voxel-based magnetic resonance morphometry
文章编号:
1005-202X（2021）07-0820-06
作者:
赵继荣1; 薛旭1; 袁毓喆2; 陈祁青1; 陈文1; 张海清1; 赵宁1; 马同1; 张天龙3; 杨涛3
1.甘肃省中医院脊柱骨科， 甘肃 兰州730050； 2.中国药科大学药学院， 江苏 南京 210000； 3.甘肃中医药大学研究生院， 甘肃 兰州 730030
Author(s):
ZHAO Jirong1;  XUE Xu1;  YUAN Yuzhe2;  CHEN Qiqing1;  CHEN Wen1;  ZHANG Haiqing1;  ZHAO Ning1;  MA Tong1;  ZHANG Tianlong3;  YANG Tao3
1. Department of Spine Orthopedics, Gansu Provincial Hospital of TCM, Lanzhou 730050, China 2. School of Pharmacy, China Pharmaceutical University, Nanjing 210000, China 3. Graduate School, Gansu University of Chinese Medicine, Lanzhou 730030, China
关键词:
腰椎间盘突出症; 磁共振形态学; 机械缩足反射阈值; 热缩足反射潜伏期; 疼痛
Keywords:
Keywords: lumbar disc herniation magnetic resonance morphometry mechanical withdrawal threshold thermal withdrawal latency pain
分类号:
R318；R683.2
DOI:
DOI:10.3969/j.issn.1005-202X.2021.07.006
文献标志码:
A
摘要:
【摘要】目的：利用基于体素的磁共振形态学（VBM）和疼痛行为学观察腰椎间盘突出症（LDH）慢性下肢痛大鼠模型脑结构及形态改变的相关性及特点，揭示LDH慢性下肢痛的脑机制。方法：选用SD雄性大鼠24只，6~8周龄，体质量（250±20） g，随机分为3组，即正常组（Normal组）、假手术组（Sham-LDH组）和模型组（LDH组），采用自体髓核移植法建立LDH慢性下肢痛模型大鼠。各组分别于建模前和建模后第2、7、14、21、28天行疼痛行为学测定，主要包括机械缩足反射阈值（MWT）及热缩足反射潜伏期（TWL）的测定。每组随机选取4只大鼠分别于建模前和建模后第14、28天行T2加权结构图像和VBM图像采集，采用重复测量方差分析对灰质体积进行统计学分析。结果：3组大鼠建模前MWT比较，差异无统计学意义（P>0.05）；Sham-LDH组大鼠MWT建模后第2、7、14、21、28天分别与Normal组大鼠比较，差异无统计学意义（P>0.05）；LDH组大鼠MWT从建模后第2天至第14天显著降低，建模后第14天至28天，MWT稳定；LDH组大鼠MWT建模后第2、7、14、21、28天分别与Normal组大鼠和Sham-LDH组大鼠比较，差异有统计学意义（P<0.05）。TWL分析结果与MWT分析结果相似。3组大鼠不同时间点脑结构区域比较存在交互作用，差异具有统计学意义（P<0.05），具体表现为左下丘脑、左海马下托、右次级运动皮层、右纹状体、右隔区、右海马、胼胝体及双侧皮质下灰质、双侧小脑分子层这些脑区的灰质体积减少。结论：成功建立自体髓核移植型LDH慢性下肢痛大鼠模型，造模后产生一个长时程的MWT和TWL的降低，出现痛觉过敏，造模成功。利用VBM能够确切地发现LDH慢性下肢痛模型大鼠脑结构发生了形态学改变。
Abstract:
Abstract: Objective To study the correlation and characteristics of brain structural and morphological changes in rat models of chronic lower limb pain due to lumbar disc herniation (LDH) using voxel-based magnetic resonance morphometry (VBM) and pain behavioral observation, thereby revealing the brain mechanism of LDH chronic lower limb pain. Methods Twenty-four SD male rats aging 6-8 weeks old, with a body weight of (250±20) g, were selected as the research objects and randomly divided into 3 groups, namely Normal group, Sham-LDH group and LDH group and the rat model of LDH chronic lower limb pain was established by the transplantation of autologous nucleus pulposus. The pain behavioral observation was performed in each group before modeling and on the 2nd, 7th, 14th, 21st and 28th days after modeling, mainly including the measurements of mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL). Four rats in each group were randomly selected to collect T2-weighted structural images and VBM images before modeling and on the 14th and 28th days after modeling. Repeated measures analysis of variance was used for the statistical analysis of gray matter volume. Results There was no significant difference in MWT among 3 groups before modeling, without statistical differences (P>0.05). The MWT in Sham-LDH group and Normal group was similar on the 2nd, 7th, 14th, 21st and 28th days after modeling (P>0.05). In LDH group, MWT decreased significantly from the 2nd day to the 14th day after modeling, and then remained stable from the 14th day to the 28th day after modeling. On the 2nd, 7th, 14th, 21st and 28th days after modeling, the MWT in LDH group was statistically different from that in Normal group and Sham-LDH group (P<0.05). The comparison results of TWL were similar to those of MWT. There were interactions between the rat brain structures of 3 groups at different time points, and the differences were statistically significant (P<0.05). Gray matter volume was reduced in some brain regions, specifically, left hypothalamic region, left hippocampus subiculum, right secondary motor cortex, right striatum, right septal region, right cornu ammonis, corpus callosum and associated subcortical gray matter, molecular layer of the lateral cerebellum. Conclusion A rat model of LDH chronic lower limb pain is successfully established after the transplantation of autologous nucleus pulposus. Long-term reductions of MWT and TWL emerge and the hyperalgesia occurs after the modeling. The morphological changes in the brain structures of rat models of LDH chronic lower limb pain can be found using VBM.

相似文献/References:

[1]陈文敏,肖玲玲,李慧慧,等. 单侧痛腰椎间盘突出症的表面肌电信号特征[J].中国医学物理学杂志,2017,34(10):1022.[doi:DOI:10.3969/j.issn.1005-202X.2017.10.011]
　[J].Chinese Journal of Medical Physics,2017,34(7):1022.[doi:DOI:10.3969/j.issn.1005-202X.2017.10.011]
[2]张天龙,赵继荣,陈祁青,等.基于RS-fMRI功能连接分析方法探讨腰椎间盘突出症致慢性腰腿痛脑机制的研究进展[J].中国医学物理学杂志,2019,36(12):1421.[doi:DOI:10.3969/j.issn.1005-202X.2019.12.011]
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[3]陈祁青,赵继荣,蔡毅,等.基于静息态功能磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究[J].中国医学物理学杂志,2022,39(4):430.[doi:DOI:10.3969/j.issn.1005-202X.2022.04.007]
　CHEN Qiqing,ZHAO Jirong,CAI Yi,et al.Experimental study of chronic lower limb pain rats with lumbar disc herniation based on resting-state functional magnetic resonance morphology[J].Chinese Journal of Medical Physics,2022,39(7):430.[doi:DOI:10.3969/j.issn.1005-202X.2022.04.007]
[4]李兰,殷小丹,李旭雪,等.3D MERGE与3D SPACE STIR序列在腰椎间盘突出症检查中的应用比较[J].中国医学物理学杂志,2024,41(1):27.[doi:DOI:10.3969/j.issn.1005-202X.2024.01.004]
　LI Lan,YIN Xiaodan,LI Xuxue,et al.Application of 3D MERGE sequence versus 3D SPACE STIR sequence in the examination of lumbar disc herniation[J].Chinese Journal of Medical Physics,2024,41(7):27.[doi:DOI:10.3969/j.issn.1005-202X.2024.01.004]

备注/Memo

备注/Memo:
【收稿日期】2021-02-15 【基金项目】国家自然科学基金（81760877）；兰州市人才创新创业专项（2018-RC-99） 【作者简介】赵继荣，主任医师，主要从事中医药治疗退行性腰椎疾病的临床与基础研究，E-mail: zhaojirong0709@163.com 【通信作者】薛旭，E-mail: 1975938420@qq.com
更新日期/Last Update: 2021-07-26