[1]徐静,汪磊,赵俊,等.用于数字等温扩增系统的荧光图像分析技术[J].中国医学物理学杂志,2020,37(10):1255-1561.[doi:DOI:10.3969/j.issn.1005-202X.2020.10.008]
 XU Jing,WANG Lei,ZHAO Jun,et al.Fluorescence image analysis technique for digital isothermal amplification system[J].Chinese Journal of Medical Physics,2020,37(10):1255-1561.[doi:DOI:10.3969/j.issn.1005-202X.2020.10.008]
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用于数字等温扩增系统的荧光图像分析技术()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第10期
页码:
1255-1561
栏目:
医学影像物理
出版日期:
2020-10-29

文章信息/Info

Title:
Fluorescence image analysis technique for digital isothermal amplification system
文章编号:
1005-202X(2020)10-1255-07
作者:
徐静1汪磊2赵俊2花昌义2朱灵2王明龙2周正仙1
1.中国科学院合肥物质科学研究院应用技术研究所/安徽省生物医学光学仪器工程技术研究中心/安徽省医用光学诊疗技术与装备工程实验室, 安徽 合肥 230031; 2.安徽师范大学物理与电子信息学院, 安徽 芜湖 241002
Author(s):
XU Jing1 WANG Lei2 ZHAO Jun2 HUA Changyi2 ZHU Ling2 WANG Minglong2 ZHOU Zhengxian1
1. Institute of Applied Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences/Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument/Anhui Provincial Engineering Laboratory for Medical Optical Diagnosis and Treatment Technology and Instrument, Hefei 230031, China 2. School of Physics and Electronic Information, Anhui Normal University, Wuhu 241002, China
关键词:
数字等温扩增技术荧光图像微流控芯片Image J图像处理软件
Keywords:
Keywords: digital isothermal amplification technology fluorescence image microfluidic chip Image J image processing software
分类号:
R318;TP311.1
DOI:
DOI:10.3969/j.issn.1005-202X.2020.10.008
文献标志码:
A
摘要:
基于微流控芯片的数字等温扩增技术作为一种新型分子检测技术,可以通过对采集到的荧光图像进行特定的图像处理,以实现对待测目标的绝对定量分析。本研究利用本课题组研制的数字微流控芯片所采集到的荧光图像为分析对象,并结合实际应用环境,开发出配套的软件分析系统。该系统中的荧光图像处理分析部分具备图像拼接、图像倾斜校正、目标区域的截取、图像分割以及荧光亮点计数等功能。该系统能很好地满足核酸分子数检测的需求,并具有良好的人机交互性能。结果表明该系统的分析结果与Image J图像处理软件的统计结果不仅具有一致性,而且能够对检测到的结果实现自动化的计算分析,这为该款软件分析系统的广泛应用奠定了坚实的基础。
Abstract:
The digital isothermal amplification technology based on microfluidic chips, as a new molecular detection technology, can be used to specifically process the collected fluorescence image, thereby achieving the absolute quantitative analysis on the target to be tested. A supporting software analysis system is developed with the fluorescence images collected by the self-developed digital microfluidic chips as the analysis object and based on the consideration of the actual application scenario. The florescence image processing part of the developed system has such functions as image splicing, image tilt correction, intercept of target area, image segmentation and fluorescence spot counting. This analysis system can satisfy the requirement of nucleic acid molecular number detection and has good human-machine interaction performance. The analysis results obtained by the system are consistent with the statistical results of Image J image processing software, and moreover the proposed system can achieve an automatic calculation and analysis of the detected results, which lays a solid foundation for the wider application of the analysis system in the future.

备注/Memo

备注/Memo:
【收稿日期】2020-03-02 【基金项目】安徽省重大科技专项(17030801007) 【作者简介】徐静,硕士研究生,研究方向:生物分析仪器系统与软件开发,E-mail: xu951130@foxmail.com 【通信作者】周正仙,副教授,研究方向:光电检测技术研究及产品开发、信号处理技术研究及应用,E-mail: earthzzx@mail.ahnu.edu.cn
更新日期/Last Update: 2020-10-29