[1]孔靖,潘远伟,张文炳.工程化金属离子纳米囊泡的制备及其在肿瘤免疫治疗的应用[J].中国医学物理学杂志,2026,43(4):510-518.[doi:DOI:10.3969/j.issn.1005-202X.2026.04.014]
　KONG Jing,PAN Yuanwei,et al.Preparation of engineered metal ion nanovesicles and their applications in cancer immunotherapy[J].Chinese Journal of Medical Physics,2026,43(4):510-518.[doi:DOI:10.3969/j.issn.1005-202X.2026.04.014]
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工程化金属离子纳米囊泡的制备及其在肿瘤免疫治疗的应用()

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
43卷
期数:
2026年第4期
页码:
510-518
栏目:
生物材料与力学
出版日期:
2026-04-28

文章信息/Info

Title:
Preparation of engineered metal ion nanovesicles and their applications in cancer immunotherapy
文章编号:
1005-202X（2026）04-0510-09
作者:
孔靖1; 2; 潘远伟2; 张文炳1
1.武汉大学物理科学与技术学院， 湖北 武汉 430072； 2.深圳湾实验室化学生物学研究所， 广东 深圳 518132
Author(s):
KONG Jing1;  2;  PAN Yuanwei2;  ZHANG Wenbing1
1. School of Physics and Technology, Wuhan University, Wuhan 430072, China 2. Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China
关键词:
乳腺癌; 工程化纳米囊泡; 锌离子; 肿瘤免疫治疗
Keywords:
Keywords: breast cancer engineered nanovesicle zinc ion tumor immunotherapy
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2026.04.014
文献标志码:
A
摘要:
目的：制备负载锌离子的工程化纳米囊泡（Zn-CM@PD1-NVs），探讨其对小鼠乳腺癌的免疫治疗效果。方法：4T1-PD1细胞膜与锌离子络合形成负载锌离子的细胞膜碎片（Zn-CM），将Zn-CM与细胞膜悬液混合，经涡旋、超声及多级滤膜挤出后制得Zn-CM@PD1-NVs。通过流式细胞术与免疫荧光验证PD1的高表达。利用动态光散射、透射电镜、电感耦合等离子体光谱表征其理化性质。通过CCK-8实验、溶血实验、免疫荧光、血生化、血常规、H&E染色分析纳米囊泡的生物安全性。建立BALB/c小鼠4T1皮下移植瘤模型，分为对照组、PD1-NVs组和Zn-CM@PD1-NVs组，评估其抗肿瘤效果；使用流式细胞术分析肿瘤微环境免疫细胞。结果：（1）Zn-CM@PD1-NVs粒径均匀稳定，具有核壳结构；（2）体外实验显示其无明显细胞毒性；（3）体内肝肾功能指标、血常规及组织切片均无异常；（4）Zn-CM@PD1-NVs组抑制了4T1肿瘤的生长，肿瘤微环境中CD8?T细胞浸润比例提高。结论：成功制备了负载锌离子的纳米囊泡Zn-CM@PD1-NVs，表现出优异的生物稳定性与生物安全性，能够有效抑制乳腺癌的增长，促进抗肿瘤免疫作用。
Abstract:
Abstract: Objective To prepare engineered nanovesicles loaded with zinc ions (Zn-CM@PD1-NVs) and investigate their immunotherapeutic efficacy against murine breast cancer. Methods The 4T1-PD1 cell membrane was complexed with zinc ions to form zinc-loaded membrane fragments (Zn-CM) which was then mixed with a cell membrane suspension, followed by vortexing, sonication, and extrusion through polycarbonate membranes to prepare Zn-CM@PD1-NVs. High PD1 expression was confirmed by flow cytometry and immunofluorescence. The physicochemical properties were characterized using dynamic light scattering, transmission electron microscopy, and inductively coupled plasma optical emission spectroscopy. Biocompatibility was evaluated through CCK-8 assay, hemolysis test, immunofluorescence, blood biochemistry, complete blood count, and H&E staining. A 4T1 subcutaneous tumor model was developed in BALB/c mice, and 3 groups, namely control, PD1-NVs, and Zn-CM@PD1-NVs groups, were established to assess the antitumor efficacy. Additionally, tumor microenvironment immune cells were analyzed by flow cytometry. Results (1) Zn-CM@PD1-NVs exhibited uniform and stable particle size with a core-shell structure. (2) In vitro experiments showed no significant cytotoxicity. (3) No abnormalities were observed in hepatic and renal function indicators, complete blood count measures, or histopathological sections. (4) Zn-CM@PD1-NVs group significantly inhibited 4T1 tumor growth, and increased the infiltration of CD8?T cells in the tumor microenvironment. Conclusion Zn-CM@PD1-NVs is successfully prepared, demonstrating not only excellent biological stability and biocompatibility, but also effective inhibition of breast cancer growth and promotion of antitumor immunity.

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备注/Memo

备注/Memo:
【收稿日期】2025-12-10 【基金项目】广东省自然科学基金（2024A1515010267） 【作者简介】孔靖，硕士研究生，研究方向：生物医用材料，E-mail: kjsduwh@163.com 【通信作者】潘远伟，博士，副研究员，研究方向：纳米生物材料与肿瘤治疗，E-mail: panyw@szbl.ac.cn；张文炳，博士，教授，研究方向：生物分子的统计热力学，E-mail: wbzhang@whu.edu.cn
更新日期/Last Update: 2026-04-29