电场刺激对雪旺细胞增殖和迁移的影响-《中国医学物理学杂志》

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Title:: Effects of electrical field stimulation on the proliferation and migration of Schwann cells

文章编号:: 1005-202X（2025）02-0240-05

作者:: 漆景天1; 叶永平1; 徐永君2; 盛青松3; 蔡龙宇1; 胡建伟1; 张永光1; 1.福建医科大学福总临床医学院/厦门大学附属东方医院（第九〇〇医院）骨科，福建福州 350025； 2.福建医科大学福总临床医学院/厦门大学附属东方医院（第九〇〇医院）福建省移植生物学重点实验室，福建福州 350025； 3.福建医科大学福总临床医学院/厦门大学附属东方医院（第九〇〇医院）妇产科，福建福州 350025

Author(s):: QI Jingtian1; YE Yongping1; XU Yongjun2; SHENG Qingsong3; CAI Longyu1; HU Jianwei1; ZHANG Yongguang1; 1. Department of Orthopaedics, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China 2. Fujian Provincial Key Laboratory of Transplant Biology, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China 3. Department of Obstetrics and Gynecology, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China

关键词:: 电场刺激; 电刺激小室; 雪旺细胞; 迁移; 增殖; 上皮间质转化

Keywords:: Keywords: electrical field stimulation electrotaxis chamber Schwann cell migration proliferation epithelial-mesenchymal transition

分类号:: R35

DOI:: DOI:10.3969/j.issn.1005-202X.2025.02.015

文献标志码:: A

摘要:: 目的：建立雪旺细胞电场刺激模型，为研究电场刺激促进雪旺细胞增殖、迁移及上皮间质转化（EMT）机制提供基础。方法：以YC-3双极性程控电刺激器和电刺激小室构建电场刺激雪旺细胞培养系统，区分为对照（Ctrl）组和电场刺激（EF）组。EF组给予持续恒压电场刺激（100 mV/mm, 3 h），Ctrl组无电场刺激。利用CCK-8法观测电场刺激对雪旺细胞增殖的影响，利用划痕实验及Transwell迁移实验观察电场刺激对雪旺细胞迁移的影响，利用Western Blot分析细胞内E-钙黏蛋白及N-钙黏蛋白含量，观察电场刺激对雪旺细胞粘附性的影响。结果：CCK-8法测定提示EF组在450 nm吸光度显著高于Ctrl组（P<0.05）。细胞划痕及Transwell迁移实验结果提示EF组细胞迁移效率显著高于Ctrl组（P<0.05）。Western Blot结果提示EF组相较于Ctrl组，E-钙黏蛋白表达降低，N-钙黏蛋白表达增多（P<0.05）。结论：本文改进的电场刺激雪旺细胞培养系统可操作性强，电场刺激后可促进雪旺细胞增殖和迁移，并且可观察到E-钙黏蛋白表达降低，N-钙黏蛋白表达增多，可能与电场刺激后雪旺细胞发生EMT有关。

Abstract:: Objective To establish an electrical field (EF) stimulation model for Schwann cells (SCs), and to provide a basis for exploring the mechanisms of EF stimulation in promoting proliferation, migration and epithelial-to-mesenchymal transition of SCs. Methods A YC-3 bipolar programmable electrical stimulator and an electrotaxis chamber were used to construct an EF stimulation system to stimulate SCs. In the study, SCs were divided into control group (Ctrl) receiving no EF stimulation and EF group stimulated by continuous constant-voltage EF (100 mV/mm, 3 h). The effects of EF stimulation on the proliferation and migration of SCs were analyzed using CCK-8 assay, and wound healing assay + Transwell assay, separately and its effect on SCs adhesion was observed by analyzing the expressions of E-cadherin and N-cadherin using Western Blot. Results The CCK-8 assay results suggested that the absorbance at 450 nm was significantly higher in EF group than in Ctrl group (P<0.05). The results of wound healing assay + Transwell assay revealed that EF group had higher cell migration efficiency than Ctrl group (P<0.05). Western Blot results showed decreased E-cadherin expression and increased N-cadherin expression in EF group as compared with Ctrl group (P<0.05). Conclusion The improved EF stimulation system for SCs is operable. EF stimulation can promote the proliferation and migration of SCs. The decreased E-cadherin expression and increased N-cadherin expression may be related to the occurrence of epithelial-to-mesenchymal transition in SCs after EF stimulation.

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

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