[1]熊珊,申洪.IGFBP1在胃癌组织中的表达特点及其预后作用与机制的生物信息学分析[J].中国医学物理学杂志,2024,41(5):628-639.[doi:DOI:10.3969/j.issn.1005-202X.2024.05.015]
 XIONG Shan,SHEN Hong,Expression characteristics of IGFBP1 in gastric cancer and bioinformatics analysis of its prognostic effect and mechanism[J].Chinese Journal of Medical Physics,2024,41(5):628-639.[doi:DOI:10.3969/j.issn.1005-202X.2024.05.015]
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IGFBP1在胃癌组织中的表达特点及其预后作用与机制的生物信息学分析()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
41卷
期数:
2024年第5期
页码:
628-639
栏目:
医学生物信息
出版日期:
2024-05-23

文章信息/Info

Title:
Expression characteristics of IGFBP1 in gastric cancer and bioinformatics analysis of its prognostic effect and mechanism
文章编号:
1005-202X(2024)05-0628-12
作者:
熊珊1申洪12
1.华南理工大学医学院, 广东 广州 510006; 2.南方医科大学基础医学院病理学系, 广东 广州 510515
Author(s):
XIONG Shan1 SHEN Hong1 2
1. School of Medicine, South China University of Technology, Guangzhou 510006, China 2. Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China
关键词:
胃癌IGFBP1免疫细胞浸润生物信息预后
Keywords:
Keywords gastric cancer IGFBP1 immune cell infiltration bioinformatics prognosis
分类号:
R318.04;R735.2
DOI:
DOI:10.3969/j.issn.1005-202X.2024.05.015
文献标志码:
A
摘要:
目的:通过综合方法分析IGFBP1在胃癌表达、预后以及参与的生物学功能,探讨其在胃癌中的诊断和治疗价值。方法:基于TCGA数据分析IGFBP1在胃癌中的表达水平,以及不同临床病理分期和生存状态下的表达差异;采用Kaplan-Meier曲线描述IGFBP1表达和胃癌患者预后的关系;采用ROC曲线、Cox回归及Meta分析评价IGFBP1在胃癌中诊断及预后价值,利用LASSO回归分析建立与IGFBP1相关的预后模型,并通过PPI网络筛选IGFBP1相关的核心基因;利用GO、KEGG及CIBERSORT等分析方法分析IGFBP1在胃癌中涉及的潜在致病通路及肿瘤免疫浸润;通过Cell Miner数据库预测IGFBP1与抗肿瘤药物敏感性的相关性。结果:IGFBP1在多种癌症中异常表达,包括胃癌,其中在III~IV期胃癌患者中表达显著上调;Cox回归证实IGFBP1是独立危险因素,预后分析提示IGFBP1与预后不良密切相关;GO分析提示IGFBP1相关差异基因在细胞粘附的正向调节、囊泡管腔和丝氨酸水解酶活性富集,KEGG分析提示其在IL-17信号通路中富集;肿瘤免疫浸润分析提示IGFBP1高低表达组间免疫细胞浸润显著差异;单因素Cox和LASSO回归分析构建了IGFBP1相关的胃癌预后模型,PPI网络从中筛选出7个核心基因,这些基因在胆固醇代谢、嘧啶代谢和PPAR信号通路中富集;IGFBP1的表达水平与Dasatinib等药物的敏感性相关。结论:IGFBP1在胃癌中表达上调并导致患者预后不良,可能通过影响IL-17信号通路和M0巨噬细胞浸润等机制调控胃癌发生发展。
Abstract:
Abstract: Objective To analyze IGFBP1 expression in gastric cancer, prognosis and involved biological functions through comprehensive approach, and to explore its diagnostic and therapeutic value in gastric cancer. Methods The expression level of IGFBP1 in gastric cancer was obtained using TCGA data, and the differences of expression in different clinicopathological stages and survival states were analyzed. Kaplan-Meier curve was used to describe the relationship between IGFBP1 expression and prognosis of gastric cancer patients. The diagnostic and prognostic value of IGFBP1 in gastric cancer was evaluated using ROC curve, Cox regression and Meta-analysis. A prognostic model associated with IGFBP1 was established after LASSO regression analysis, and the IGFBP1-related core genes were identified in PPI network. GO, KEGG and CIBERSORT were used to analyze the potential pathogenic pathways and immune cell infiltration associated with IGFBP1 in gastric cancer. The correlation between IGFBP1 and anti-tumor drug sensitivity was predicted based on Cell Miner database. Results IGFBP1 was abnormally expressed in a variety of cancers, including gastric cancer, and its expression was significantly up-regulated in patients with stage III-IV gastric cancer. Cox regression identified IGFBP1 as an independent risk factor, and prognostic analysis suggested that IGFBP1 was closely associated with bleak prognosis. GO analysis indicated that IGFBP1-related differential genes were enriched in positive regulation of cell adhesion, vesicle lumen, and serine hydrolase activity while KEGG analysis suggested that they were enriched in IL-17 signaling pathway. The analysis of tumor-associated immune cell infiltration showed significant differences in immune cell infiltration between high and low IGFBP1 expression groups. IGFBP1 related gastric cancer prognosis model was constructed using univariate Cox and LASSO regression analyses. Seven core genes were identified in PPI network, enriching in cholesterol metabolism, pyrimidine metabolism, and PPAR signaling pathway. The expression level of IGFBP1 was correlated with the sensitivity to Dasatinib and other drugs. Conclusion The up-regulated expression of IGFBP1 in gastric cancer leads to poor prognosis, which may regulate the occurrence and development of gastric cancer by affecting IL-17 signaling pathway and M0 macrophage infiltration.

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备注/Memo

备注/Memo:
【收稿日期】2024-02-15 【基金项目】国家自然科学基金(30271462) 【作者简介】熊珊,硕士研究生,医师,E-mail: 790905102@qq.com 【通信作者】申洪,主任医师,教授,博士生导师,研究方向:病理学,E-mail: shenhong2010168@163.com
更新日期/Last Update: 2024-05-24