不同光谱LED对视锥细胞内质网应激的影响-《中国医学物理学杂志》

文章信息/Info

Title:: Effects of different spectral LEDs on endoplasmic reticulum stress in cone cells

作者:: 陈文奕1; 林蓉1; 肖昆鸿1; 袁坤2; 陈重江1; 黄焱1; 1.福建医科大学医学技术与工程学院眼视光学系，福建福州 350004； 2.北京协和医学院阜外医院国家心血管病中心社区防治部，北京 100000

Author(s):: CHEN Wenyi1; LIN Rong1; XIAO Kunhong1; YUAN Kun2; CHEN Zhongjiang1; HUANG Yan1; 1. Department of Ophthalmic Optics, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China 2. Division of Prevention and Community Health, Fuwai Hospital and National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100000, China

Keywords:: light emitting diode sun-like spectrum cone cell endoplasmic reticulum stress

摘要:: 目的：观察类太阳光谱发光二极管（LED）和常规白光LED对小鼠来源视锥细胞（661W）内质网应激的影响。方法：将661W细胞分为无光照对照组（NC组）、3 000 lux类太阳光谱LED照射6~24 h组（SL组）、3 000 lux常规白光LED照射6~24 h组（CL组）。采用光学显微镜观察细胞状态，CCK-8检测细胞活力，实时荧光定量PCR（RT-qPCR）和Western blot 检测内质网应激PERK通路相关基因和蛋白表达水平。结果：与NC组相比，光照组细胞死亡率随时间递增，细胞活力值均明显降低（P<0.05），SL组细胞损伤程度轻于CL组；CL照射后ATF4和CHOP的mRNA水平和ATF4蛋白水平增加（P<0.01）；GRP78/Bip的mRNA和蛋白水平在CL-6 h组增加（P<0.01）后下降；而SL-24 h组增加了ATF4的蛋白表达水平（P<0.05）。结论：常规白光LED可导致内质网应激，引起视锥细胞光损伤，类太阳光谱LED引起的反应明显减轻。

Abstract:: Abstract: Objective To investigate the endoplasmic reticulum (ER) stress in mouse cone cells (661W cells) exposed to sun-like spectral LED and conventional white LED. Methods 661W cells were divided into normal control group (NC) for cell culture in darkness, SL groups for 3 000 lux sun-like spectral LED exposure for 6-24 h, and CL groups for 3 000 lux conventional white LED exposure for 6-24 h. Cell state was observed using optical microscopy, and CCK-8 assay was used to detect cell viability. The ER stress-related genes and protein expression levels were detected with real-time fluorescence quantification PCR (RT-qPCR) and Western blot. Results Compared with that in NC group, the rate of cell death in LED exposure groups was increased with time, and the cell viability was decreased significantly (P<0.05), but the cell damages were lighter in SL groups than in CL groups. CL exposure increased the expression levels of ATF4 mRNA, CHOP mRNA and ATF4 protein (P<0.01). The expression levels of GRP78/Bip mRNA and protein were significantly increased in CL-6 h group (P<0.01), and then decreased significantly. SL-24 h group improved the expression levels of ATF4 protein (P<0.05). Conclusion Conventional white LED can result in ER stress and induce cone cell damage, but the responses caused by sun-like spectral LED is significantly reduced.