加载PEDF的多聚体超声微泡研制及其特性-《中国医学物理学杂志》

文章信息/Info

Title:: Preparation?f PEDF-loaded polymer ultrasound microbubbles and its properties

作者:: 胡劼1; 2; 焦明克3; 刘立洁3; 张鹏3; 牛盈盈3; 1.新疆医科大学第四附属医院心脏超声科，新疆乌鲁木齐 830000； 2.重庆市渝北区人民医院超声科，重庆 400000； 3.新疆军区总医院医学工程科，新疆乌鲁木齐 830000

Author(s):: HU Jie1; 2; JIAO Mingke3; LIU Lijie3; ZHANG Peng3; NIU Yingying3; 1. Department of Echocardiography, the Fourth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China 2. Department of Ultrasound, Yubei District Peoples Hospital of Chongqing, Chongqing 400000, China 3. Department of Biomedical Engineering, General Hospital of Xinjiang Military Region, Urumqi 830000, China

Keywords:: Keywords: angiogenic disease pigm entepithelium-derived factor ultrasound microbubble targeted treatment

摘要:: 目的：针对血管新生类疾病，研制与色素上皮源因子（PEDF）具有最大结合率的多聚体超声微泡，并明确其理化特性。方法：首先应用声空化方法制备多聚体超声微泡；然后将PEDF按浓度分为0.4、2.0、10.0和50.0 μg /mL组，应用跨膜按梯度法包裹入脂质体，使其与超声微泡结合，进而置于荧光显微镜下观察。应用流式细胞仪检测PEDF与超声微泡的最大结合率，采用荧光分光光度法测定载药超声微泡的包封率及体外释药特性。结果：成功制备了与PEDF最大结合率为（96.14±1.21）%的多聚体超声微泡。脂质体对PEDF的包封率约为（79.20±2.31）%，体外释放浓度于微泡击破后1 min内达到峰值，持续释放10 min后浓度显著减低。结论：与PEDF具有较高结合率的多聚体超声微泡可以作为合适的药物载体，靶向治疗血管新生类疾病。

Abstract:: Abstract: Objective To develop the polymer ultrasound microbubbles with the maximum binding rate to pigm entepithelium-derived factor (PEDF) for angiogenic diseases, and to clarify the physical and chemical properties. Methods Polymer ultrasound microbubbles were prepared by acoustic cavitation method. After PEDF was divided into different groups (0.4, 2.0, 10.0 and 50.0 μg/mL) according to the concentration, it was encapsulated with liposomes using transmembrane gradient method, which enabled PEDF to bind to ultrasound microbubbles, and then the binding was observed under fluorescence microscope. The maximum binding rate of PEDF to ultrasound microbubbles was obtained by flow cytometry. Moreover, the efficiency of encapsulating PEDF into liposomes and the in vitro drug release characteristics of the drug-loaded ultrasound microbubbles were determined by fluorescence spectrophotometry. Results The polymer ultrasound microbubbles which had a maximum binding rate of (96.14±1.21)% to PEDF was successfully prepared. The efficiency of encapsulating PEDF into liposomes was about (79.20±2.31)%. The in vitro drug release concentration reached the maximum in 1 min after the microbubble breaking, and the concentration was obviously reduced after the continuous release for 10 min. Conclusion The polymer ultrasound microbubbles with a high binding rate to PEDF can be used as a suitable drug loader for targeted treatment of angiogenic diseases.