[1]张宇,马成华,景鹏举,等.低强度脉冲超声通过PI3K/AKT/mTOR信号通路抑制骨肉瘤细胞的增殖[J].中国医学物理学杂志,2021,38(3):355-359.[doi:DOI:10.3969/j.issn.1005-202X.2021.03.017]
　ZHANG Yu,MA Chenghua,JING Pengju,et al.Low-intensity pulsed ultrasound inhibits the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway[J].Chinese Journal of Medical Physics,2021,38(3):355-359.[doi:DOI:10.3969/j.issn.1005-202X.2021.03.017]
点击复制

低强度脉冲超声通过PI3K/AKT/mTOR信号通路抑制骨肉瘤细胞的增殖()
分享到：

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第3期
页码:
355-359
栏目:
医学生物物理
出版日期:
2021-03-30

文章信息/Info

Title:
Low-intensity pulsed ultrasound inhibits the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway
文章编号:
1005-202X（2021）03-0355-05
作者:
张宇; 马成华; 景鹏举; 李培武
兰州大学第二医院急救中心， 甘肃 兰州 730000
Author(s):
ZHANG Yu;  MA Chenghua;  JING Pengju;  LI Peiwu
Emergency Center, Lanzhou University Second Hospital, Lanzhou 730000, China
关键词:
低强度脉冲超声; 磷脂酰肌醇3-激酶; 骨肉瘤; 增殖; 信号通路
Keywords:
Keywords: low-intensity pulsed ultrasound phosphatidylinositol 3-kinase osteosarcoma proliferation signaling pathway
分类号:
R363；R738.1
DOI:
DOI:10.3969/j.issn.1005-202X.2021.03.017
文献标志码:
A
摘要:
目的：阐述低强度脉冲超声（LIPUS）对骨肉瘤细胞增殖的调控作用和机制，探索低强度脉冲超声治疗骨肉瘤的潜在价值。方法：在时间梯度实验中，将MG-63细胞分为LIPUS组和Control组，对LIPUS组加载1、6、12、18和24 h的低强度脉冲超声，CCK-8检测各组细胞增殖活力，Western Blots检测24 h低强度脉冲超声对PI3K/AKT/mTOR信号通路的影响。在机制实验中，将MG-63细胞分为Control组、LY294002组、LIPUS组和LIPUS+740Y-P组，对各组施加对应的干预措施后使用CCK-8检测各组细胞增殖能力，Western Blots检测各组增殖相关蛋白PCNA和Cyclin D1的表达情况。所有数据重复3次后使用单因素方差分析统计。结果：加载低强度脉冲超声18和24 h后，LIPUS组的细胞增殖活力较对照组显著降低（P<0.010, P<0.001），这些结果表明LIPUS可抑制MG-63细胞的增殖。LIPUS可抑制p-PI3K、p-AKT和p-mTOR的表达（P<0.001），即抑制PI3K/AKT/mTOR信号通路的激活，而不影响PI3K、AKT和mTOR的表达。使用LIPUS就如同使用PI3K的抑制剂（LY294002）一样，可以抑制MG-63细胞的增殖（P<0.001），在使用PI3K的激动剂740Y-P后，可逆转LIPUS对MG-63细胞增殖的抑制（P<0.001）。这些结果表明LIPUS可以通过抑制PI3K/AKT/mTOR信号通路的激活来抑制骨肉瘤细胞的增殖。结论：低强度脉冲超声通过PI3K/AKT/mTOR信号通路抑制骨肉瘤细胞的增殖，它可能是单独或联合放化疗治疗骨肉瘤的有效方法之一。
Abstract:
Abstract: Objective To clarify the role and mechanism of low-intensity pulsed ultrasound (LIPUS) in regulating the proliferation of osteosarcoma cells, and to explore the potential value of LIPUS in the treatment of osteosarcoma. Methods In time gradient experiment, MG-63 cells were divided into LIPUS group and control group. MG-63 cells in LIPUS group were loaded with LIPUS for 1, 6, 12, 18 and 24 h. CCK-8 was used for detecting the cell proliferation in each group, and Western Blots for detecting the effects of 24 h LIPUS on PI3K/AKT/mTOR signaling pathway. In mechanism experiment, MG-63 cells were divided into control group, LY294002 group, LIPUS group and LIPUS+740Y-P group. After treating MG-63 cells with corresponding interventions in different groups, CCK-8 was used for detecting cell proliferation in each group, and Western Blots for detecting the expressions of proliferation-related proteins PCNA and Cyclin D1. After all data were repeated 3 times, one-way ANOVA was adopted for statistical analysis. Results After loading LIPUS for 18 and 24 h, the cell proliferation in LIPUS group was significantly lower than that in control group (P<0.010, P<0.001), which indicated that LIPUS could inhibit the proliferation of MG-63 cells. In addition, LIPUS could inhibit the expressions of p-PI3K, p-AKT and p-mTOR (P<0.001) (inhibiting the activation of PI3K/AKT/mTOR signaling pathway), without affecting the expressions of PI3K, AKT and mTOR. Like the inhibitor of PI3K (LY294002), LIPUS could inhibit the proliferation of MG-63 cells (P<0.001). The inhibition of LIPUS on proliferation of MG-63 cells could be reversed by 740Y-P (the agonist of PI3K) (P<0.001). These results revealed that LIPUS could inhibit the proliferation of osteosarcoma cells by inhibiting the activation of PI3K/AKT/mTOR signaling pathway. Conclusion LIPUS can inhibit the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway. LIPUS combined with chemotherapy or not may be one of the effective methods for the treatment of osteosarcoma.

相似文献/References:

[1]郭霜,李选鹏,王华彬,等. 低强度脉冲超声治疗的应用进展[J].中国医学物理学杂志,2018,35(8):927.[doi:DOI:10.3969/j.issn.1005-202X.2018.08.012]
　GUO Shuang,LI Xuanpeng,WANG Huabin,et al. Advances in application of low-intensity pulsed ultrasound therapy[J].Chinese Journal of Medical Physics,2018,35(3):927.[doi:DOI:10.3969/j.issn.1005-202X.2018.08.012]
[2]郭霜,满江位,姜春倩,等. 低强度脉冲超声的生物物理学效应及相关机制的研究进展[J].中国医学物理学杂志,2019,36(5):605.[doi:DOI:10.3969/j.issn.1005-202X.2019.05.022]
　GUO Shuang,MAN Jiangwei,JIANG Chunqian,et al. Progress in biophysical effects of low-intensity pulsed ultrasound and related mechanisms[J].Chinese Journal of Medical Physics,2019,36(3):605.[doi:DOI:10.3969/j.issn.1005-202X.2019.05.022]

备注/Memo

备注/Memo:
【收稿日期】2020-11-19 【基金项目】国家自然科学基金（81672207） 【作者简介】张宇，住院医师，从事骨肿瘤研究，E-mail: zhangyuldey2020@yeah.net 【通信作者】李培武，主任医师，从事骨肿瘤研究，E-mail: lipeiwu2019@sohu.com
更新日期/Last Update: 2021-03-30