[1]周琴,吕国荣,郭海欣,等.利用剪切波弹性成像技术评价大鼠非酒精性脂肪性肝病严重程度[J].中国医学物理学杂志,2021,38(8):955-959.[doi:DOI:10.3969/j.issn.1005-202X.2021.08.007]
　ZHOU Qin,L?Guorong,GUO Haixin,et al.Evaluating the severity of nonalcoholic fatty liver disease in rats by shear wave elastography[J].Chinese Journal of Medical Physics,2021,38(8):955-959.[doi:DOI:10.3969/j.issn.1005-202X.2021.08.007]
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利用剪切波弹性成像技术评价大鼠非酒精性脂肪性肝病严重程度()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
38卷
期数:
2021年第8期
页码:
955-959
栏目:
医学影像物理
出版日期:
2021-08-02

文章信息/Info

Title:
Evaluating the severity of nonalcoholic fatty liver disease in rats by shear wave elastography
文章编号:
1005-202X（2021）08-0955-05
作者:
周琴1; 吕国荣1; 2; 郭海欣1; 李靖云2; 张少波3; 陈永健1
1.福建医科大学附属第二医院超声科， 福建 泉州 362000； 2.泉州医学高等专科学校临床医学院， 福建 泉州 362000； 3.晋江市安海医院超声科， 福建 泉州 362000
Author(s):
ZHOU Qin1;  L?Guorong1; 2;  GUO Haixin1;  LI Jingyun2;  ZHANG Shaobo3;  CHEN Yongjian1
1. Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China 2. School of Clinical?edicine, Quanzhou Medical College, Quanzhou 362000, China 3. Department of Ultrasound, Jinjiang Anhai Hospital, Quanzhou 362000, China
关键词:
非酒精性脂肪性肝病; 剪切波; 弹性成像技术; 生物分子; 杨氏模量
Keywords:
Keywords: nonalcoholicai fatty liver disease shear wave elastography biomolecule Youngs modulus
分类号:
R365
DOI:
DOI:10.3969/j.issn.1005-202X.2021.08.007
文献标志码:
A
摘要:
目的：探讨剪切波弹性成像（SWE）技术评估非酒精性脂肪性肝病（NAFLD）严重程度的可行性及其影响因素。方法：SD大鼠随机分为对照组（40只）和实验组（48只），实验组通过不同饮食建立严重程度不同的NAFLD大鼠模型，分别于第1、2、3、8、12周末检测肝脏杨氏模量平均值（Emean）并切除大鼠肝脏行组织病理学和mRNA表达分析。结果：Emean与NAFLD严重程度呈显著正相关（r=0.838, P<0.001）。NAFLD不同病理分组间的Emean差异有统计学意义（F=113.58, P<0.001）。非酒精性脂肪性肝炎（NASH）组和肝硬化组Emean显着高于正常组、单纯性脂肪变性和临界组（P<0.001）；肝硬化组的Emean显着高于NASH组（P<0.001）；正常组、单纯性脂肪变性组和临界组之间，Emean没有显着差异（P>0.05）。Emean与MCP-1、TNF-α、Ⅰ型胶原和α-SMA表达水平均呈显著正相关（r=0.340~0.678, P均<0.01），而MCP-1（B=0.560, P<0.001）、I型胶原（B=0.429, P<0.001）是与肝脏Emean呈独立相关的因素。。SWE诊断NASH及肝硬化的截值分别为6.0 kPa、9.7 kPa，曲线下面积分别为0.95、0.98，敏感性分别为88.9%、86.5%，特异性分别为100%、92.7%。结论：SWE技术可用于评估NAFLD严重程度；MCP-1、I型胶原是影响肝脏硬度的主要生物分子。
Abstract:
Abstract: Objective To explore the feasibility and influencing factors of shear wave elastography (SWE) technique in assessing the severity of nonalcoholicai fatty liver disease (NAFLD). Methods SD rats were randomly divided into control group (n=40) and experimental group (n=48). The rat models with various degrees of NAFLD severity in experimental group were created by different diets. The mean Youngs modulus (Emean) of the livers was detected at the end of the 1st, 2nd, 3rd, 8th and 12th weeks, and finally the livers were excised for histopathologic evaluation and mRNA expression analysis. Results Emean was positively correlated with NAFLD severity in all rats (r=0.838, P<0.001). There was statistical difference in Emean among different pathological groups (F=113.58, P<0.001). Emean in nonalcoholic steatohepatitis (NASH) and cirrhosis groups was significantly higher than that in normal, simple steatosis and borderline groups (P<0.001). Emean of cirrhosis group was also significantly higher than that of NASH group (P<0.001), and no significant difference was found in Emean among normal group, simple steatosis group and borderline group (P>0.05). The mRNA expressions of MCP-1, TNF-α, type I collagen and α-SMA had significant positive correlations with Emean (r=0.340-0.678, all P<0.001). Among them, MCP-1 (B=0.560, P<0.001) and type I collagen (B=0.429, P<0.001) were independent risk factors for NAFLD. The optimal SWE cutoff values for the diagnosis of NASH and cirrhosis were 6.0 and 9.7 kPa, respectively, with the area under curve of 0.95 and 0.98, sensitivity of 88.9% and 86.5%, and specificity of 100% and 92.7%, respectively. Conclusion SWE technique can be used to assess the severity of NAFLD. MCP-1 and type I collagen are the major biomolecular indicators that affects liver stiffness.

备注/Memo

备注/Memo:
【收稿日期】2021-02-20 【基金项目】福建省临床重点专科建设项目（2017（739）） 【作者简介】周琴，在读硕士，研究方向：超声诊断，E-mail: zhouqin_0506@sina.com 【通信作者】吕国荣，E-mail: lgr_feus@sina.com
更新日期/Last Update: 2021-07-30