[1]王甜,王欢博,安广洲,等.大气压冷等离子体对肿瘤细胞增殖和凋亡的影响及其机制[J].中国医学物理学杂志,2020,37(6):769-773.[doi:DOI:10.3969/j.issn.1005-202X.2020.06.021]
　WANG Tian,WANG Huanbo,et al.Effects of cold atmospheric plasma on the proliferation and apoptosis of tumor cells and its mechanisms[J].Chinese Journal of Medical Physics,2020,37(6):769-773.[doi:DOI:10.3969/j.issn.1005-202X.2020.06.021]
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大气压冷等离子体对肿瘤细胞增殖和凋亡的影响及其机制()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第6期
页码:
769-773
栏目:
医学生物物理
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of cold atmospheric plasma on the proliferation and apoptosis of tumor cells and its mechanisms
文章编号:
1005-202X（2020）06-0769-05
作者:
王甜1; 2; 王欢博1; 3; 安广洲1; 张俊平1; 4; 张克英1; 周艳1; 李静5; 郭玲1; 李和平5; 丁桂荣1
1.空军军医大学军事预防医学系/辐射防护医学教研室， 陕西 西安 710032； 2.西安市中心医院神外微创及转化医学中心， 陕西 西安 710032； 3.空军军医大学基础医学院学员队， 陕西 西安 710032； 4.中国人民解放军61255部队， 山西 侯马 043000； 5.清华大学工程物理系， 北京 100084
Author(s):
WANG Tian1;  2;  WANG Huanbo1;  3;  AN Guangzhou1;  ZHANG Junping1;  4;  ZHANG Keying1;  ZHOU Yan1;  LI Jing5;  GUO Ling1;  LI Heping5;  DING Guirong1
1. Department of Radiation Protection Medicine/Faculty of Military Preventive Medicine, Air Force Medical University, Xian 710032, China 2. Department of Minimally Invasive Neurosurgery and Translational Medicine, Xian Central Hospital, Xian 710032, China 3. Cadet Company, School of Basic Medicine, Air Force Medical University, Xian 710032, China 4. Military Health Team of 61255 Troops of the Chinese Peoples Liberation Army, Houma 043000, China 5. Department of Engineering Physics, Tsinghua University, Beijing 100084, China
关键词:
大气压冷等离子体; 肿瘤细胞; 细胞增殖; 凋亡; 氧化应激
Keywords:
Keywords: cold atmospheric plasma tumor cell cell proliferation apoptosis oxidative stress
分类号:
R35；R730.2
DOI:
DOI:10.3969/j.issn.1005-202X.2020.06.021
文献标志码:
A
摘要:
目的：探讨大气压冷等离子体（CAP）对人肿瘤细胞增殖和凋亡的影响。方法：将处于对数生长期的人神经胶质瘤细胞（U87）和宫颈癌细胞（Hela）接种于培养板或培养皿中，待细胞贴壁生长后，将其随机分为正常对照（control）组、氦气（He）组和CAP不同时间处理组，细胞在接受不同时间CAP处理后，采用CCK-8方法检测细胞活力，流式细胞仪检测细胞凋亡情况，蛋白印迹法检测凋亡相关蛋白caspase3和氧化应激相关蛋白Nrf2的表达水平。结果：He处理对U87和Hela细胞的增殖能力和自发凋亡无明显影响，CAP处理后两种肿瘤细胞的细胞活力均显著降低，凋亡水平明显上升，caspase3蛋白和Nrf2蛋白的表达显著增加，CAP处理时间越长，效应越明显。结论：本实验条件下的CAP处理可抑制人肿瘤细胞的增殖，并诱导其凋亡，其机制可能与CAP导致的氧化应激有关。
Abstract:
Abstract: Objective To explore the effects of cold atmospheric plasma (CAP) on the proliferation and apoptosis of human tumor cells. Methods Human glioma cells (U87) and cervical cancer cells (Hela) which were in logarithmic growth phase were inoculated in culture plate or culture dish. When the cells grew?dhering?o?he?all, they were randomly divided into control group, helium (He) group and CAP groups with different treatment durations. After different treatments, the cell viability was detected by CCK-8 method, and apoptosis was tested by flow cytometry, and the expression levels of caspase-3 protein related to apoptosis and Nrf2 protein related to oxidative stress were measured by Western blot. Results He treatment did not affect the proliferation and apoptosis of U87 cells and Hela cells. Compared with those in control group and He group, the viability of U87 cells and Hela cells in CAP group were significantly decreased, while the apoptosis rate was obviously increased. Moreover, the expression levels of caspase3 protein and Nrf2 protein in CAP group were remarkably increased, and the increase was more obvious with the longer duration of CAP treatment. Conclusion CAP can inhibit the proliferation of human tumor cells and induce apoptosis under this experimental condition, and the oxidative stress might be involved in this process.

相似文献/References:

[1]张克英,陈启亮,芮刚,等. 大气压冷等离子体对大鼠凝血功能的影响[J].中国医学物理学杂志,2019,36(3):351.[doi:DOI:10.3969/j.issn.1005-202X.2019.03.021]
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备注/Memo

备注/Memo:
【收稿日期】2020-01-16 【基金项目】陕西省社会发展科技攻关项目（2016SF-018） 【作者简介】王甜，硕士研究生，研究方向：生物电磁学，E-mail: scuwangtian1989@sina.cn 【通信作者】丁桂荣，博士生导师，研究方向：生物电磁学，E-mail: dingzhao@fmmu.edu.cn；李和平，博士生导师，研究方向：等离子体科学与技术，E-mail: liheping@tsinghua.edu.cn
更新日期/Last Update: 2020-07-03