[1]敖经盛,鲍志荣,全红.纳米复合材料GO@AgPt对非小细胞肺癌的放射增敏作用[J].中国医学物理学杂志,2020,37(4):514-519.[doi:DOI:10.3969/j.issn.1005-202X.2020.04.021]
　AO Jingsheng,BAO Zhirong,et al.Radiosensitizing effect of GO@AgPt nanocomposite on non-small-cell lung cancer[J].Chinese Journal of Medical Physics,2020,37(4):514-519.[doi:DOI:10.3969/j.issn.1005-202X.2020.04.021]
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纳米复合材料GO@AgPt对非小细胞肺癌的放射增敏作用()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第4期
页码:
514-519
栏目:
生物材料与力学
出版日期:
2020-04-29

文章信息/Info

Title:
Radiosensitizing effect of GO@AgPt nanocomposite on non-small-cell lung cancer
文章编号:
1005-202X（2020）04-0514-06
作者:
敖经盛1; 2; 鲍志荣1; 全红2
1.武汉大学中南医院放化疗科， 湖北 武汉430071； 2.武汉大学物理科学与技术学院， 湖北 武汉 430072
Author(s):
AO Jingsheng1;  2;  BAO Zhirong1;  QUAN Hong2
1. Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China; 2. School of Physics and Technology, Wuhan University, Wuhan 430072, China
关键词:
非小细胞肺癌; 纳米复合材料; 放射增敏
Keywords:
Keywords: non-small-cell lung cancer;  nanocomposite;  radiosensitization
分类号:
R318.08；R734.2
DOI:
DOI:10.3969/j.issn.1005-202X.2020.04.021
文献标志码:
A
摘要:
目的：制备新型纳米复合材料GO@AgPt，探究其对非小细胞肺癌的放射增敏效果。方法：通过改进型的水热法合成纳米复合材料GO@AgPt，通过透射电子显微镜、原子力显微镜、X射线光电子能谱分析、傅里叶红外光谱仪等方法对材料进行形貌及组分分析。应用细胞活性检测试剂盒CCK-8检测材料对肺癌细胞A549和人肺微血管细胞HPMEC的细胞活性的影响；然后通过流式检测及细胞克隆形成实验探究材料对于A549细胞的放射增敏效果。结果：成功制备出粒径大小均匀、化学成分稳定的纳米复合材料GO@AgPt。在一定浓度范围内该材料对肺癌细胞A549有毒性而对正常细胞HPMEC没有明显毒性。该材料能促进癌细胞产生活性氧，与X射线联合作用能促进细胞的凋亡。细胞克隆形成的结果也显示该材料对肺癌细胞具有放射增敏效果。结论：成功制备了纳米复合材料GO@AgPt，证明了该材料对于非小细胞肺癌有明显的放疗增敏效果。
Abstract:
Abstract: Objective To prepare a novel GO@AgPt nanocomposite as a new radiotherapy sensitizer and investigate its radiosensitizing effects on non-small-cell lung cancer (NSCLC). Methods GO@AgPt nanocomposite was prepared by an improved hydrothermal method. Transmission electron microscope, atomic force microscope, X-ray photoelectron spectroscopy and Fourier transform infrared spectrometer were utilized to characterize the surface morphology of GO@AgPt nanocomposite and perform an analysis on the chemical compositions. The cytotoxicities of GO@AgPt nanocomposite on NSCLC cells (A549 cells) and human pulmonary microvascular endothelial cells (HPMEC cells) were evaluated by cell counting kit-8 (CCK-8) assay, and the radiosensitization effects of GO@AgPt nanocomposite on A549 cells was determined by colony formation test and flow cytometer. Results GO@AgPt nanocomposite with uniform particle size and stable chemical compositions was successfully prepared. In a certain concentration range, GO@AgPt nanocomposite had a toxic effect on A549 cells, and meanwhile HPMEC cells were not affected. The nanocomposite enhanced the reactive oxygen production in A549 cells, and the combination with X-ray promoted cell apoptosis. Furthermore, the colony formation test showed that GO@AgPt nanocomposite had radiosensitizing effects on A549 cells. Conclusion GO@AgPt nanocomposite is successfully prepared and is proved to be a promising radiotherapy sensitizer for NSCLC.

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备注/Memo

备注/Memo:
【收稿日期】2019-11-11 【基金项目】湖北省卫生健康科研基金（WJ2019H076） 【作者简介】敖经盛，硕士，研究方向：纳米医学，E-mail:sunny_whu@163.com 【通信作者】鲍志荣，硕士，研究员，研究方向：放射物理、纳米材料，E-mail: bao_zhirong@163.com；全红，博士，副教授，研究方向：医学物理、核医学，E-mail: csp6606@sina.com
更新日期/Last Update: 2020-04-29