[1]黄毓娟,万里,杨志宏,等.雄激素受体αA基因甲基化和tHcy对颅内动脉狭窄的影响[J].中国医学物理学杂志,2020,37(3):311-316.[doi:DOI:10.3969/j.issn.1005-202X.2020.03.011]
　HUANG Yujuan,WAN Li,YANG Zhihong,et al.Effects of methylation of androgen reciptor-αA gene and total homocysteinet on intracranial arterial stenosis[J].Chinese Journal of Medical Physics,2020,37(3):311-316.[doi:DOI:10.3969/j.issn.1005-202X.2020.03.011]
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雄激素受体αA基因甲基化和tHcy对颅内动脉狭窄的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第3期
页码:
311-316
栏目:
医学影像物理
出版日期:
2020-03-25

文章信息/Info

Title:
Effects of methylation of androgen reciptor-αA gene and total homocysteinet on intracranial arterial stenosis
文章编号:
1005-202X（2020）03-0311-06
作者:
黄毓娟1; 万里2; 杨志宏3; 沈舒文1; 4
1.陕西中医药大学基础医学院， 陕西 咸阳 712046； 2.西安大兴医院神经内科， 陕西 西安 710016； 3.陕西中医药大学附属医院脑病科， 陕西 咸阳 712046； 4.陕西中医药大学中医系， 陕西 咸阳 712046
Author(s):
HUANG Yujuan1;  WAN Li2;  YANG Zhihong3;  SHEN Shuwen1;  4
1. School of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China; 2. Department of Neurology, Xi’an Daxing Hospital, Xi’an 710016, China; 3. Department of Encephalopathy, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712046, China; 4.School of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
关键词:
雄激素受体αA; 甲基化; 总同型半胱氨酸; 颅内动脉狭窄
Keywords:
Keywords: androgen receptor-αA;  methylation;  total homocysteinet;  intracranial artery stenosis
分类号:
R35；R445.2
DOI:
DOI:10.3969/j.issn.1005-202X.2020.03.011
文献标志码:
A
摘要:
目的：主要探讨人雄激素受体αA（Androgen reciptor-αA, AR-αA）基因启动子区甲基化状态与高分辨率磁共振成像下，以大脑中动脉M1段不同信号斑块为例，探讨颅内动脉狭窄斑块临床与分子生物学的关系。方法：在缺血性脑卒中患者中，行头颅高分辨率磁共振成像检查，入选69例大脑中动脉M1段存在斑块且有神经系统缺损症状的患者和30例对照组，评估颅内动脉斑块信号。采用甲基化特异性聚合酶链反应（MSP）检测静脉血AR-αA基因启动子区甲基化状态及荧光法测定血清总同型半胱氨酸（tHcy）水平。结果：斑块高信号或混杂信号组42例AR-αA基因启动子区甲基化率为78.6%；等信号或低信号组27例AR-αA基因启动子区甲基化率为70.4%；30例对照者中AR-αA基因启动子甲基化率为26.6%；3组甲基化率差异有显著性意义（P<0.05）。同时，实验组tHcy水平显著高于对照组（P<0.05），AR-αA基因启动子区甲基化程度与tHcy相关系数为r=0.549。结论：颅内动脉高信号斑块的不稳定性程度增加，与tHcy成正相关，更易引起颅内动脉狭窄。
Abstract:
Abstract: Objective To discuss the relationship between intracranial arterial stenosis plaques in clinic and molecular biology under the methylation state of androgen reciptor-αA (AR-αA) gene promoter region and high-resolution magnetic resonance imaging by taking plaques of different signals in M1 segment of middle cerebral artery as example. Methods Patients with ischemic stroke were detected by high-resolution magnetic resonance imaging. A total of 69 patients with plaques in M1 segment of middle cerebral artery and neurological defect symptoms were enrolled in the study, and 30 cases in control group. Intracranial arterial plaque signals were evaluated. Methylation specific polymerase chain reaction (MSP) was used for detecting methylation state of AR-αA gene promoter region of venous blood, and fluorescence method for determining the level of serum total homocysteinet (tHcy). Results The methylation rate of AR-αA gene promoter region in the high signal or mixed signal group (n=42) was 78.6%, and that was 70.4% in iso-signal or low signal group (n=27) and 26.6% in control group (n=30). There were statistical differences in methylation rate among 3 groups (P<0.05). Meanwhile, the level of tHcy in experimental group was significantly higher than that in control group (P<0.05). The correlation coefficient r between the methylation of AR-αA gene promoter region and tHcy was 0.549. Conclusion The instability of high signal plaques in intracranial artery is increased and is positively related to tHcy, which is more likely to lead to intracranial artery stenosis.

备注/Memo

备注/Memo:
【收稿日期】2019-11-21 【基金项目】全国名老中医药专家沈舒文教授传承工作室建设项目（国中医药人教发【2016】42号） 【作者简介】黄毓娟，硕士，讲师，主要从事内科疾病的诊断、治疗及科研工作，E-mail: huangyujuan197@163.com 【通信作者】万里，副主任医师，主要从事神经内科的诊断及治疗工作，E-mail: wwwwoods@163.com
更新日期/Last Update: 2020-04-02