[1]刘仁峰,陈诚,吴江怡,等.cofilin对软骨细胞骨架及表型的影响[J].中国医学物理学杂志,2020,37(1):107-114.[doi:DOI:10.3969/j.issn.1005-202X.2020.01.020]
　LIU Renfeng,CHEN Cheng,WU Jiangyi,et al.Effects of cofilin on the cytoskeleton and phenotype of chondrocytes[J].Chinese Journal of Medical Physics,2020,37(1):107-114.[doi:DOI:10.3969/j.issn.1005-202X.2020.01.020]
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cofilin对软骨细胞骨架及表型的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
37
期数:
2020年第1期
页码:
107-114
栏目:
医学生物物理
出版日期:
2020-01-25

文章信息/Info

Title:
Effects of cofilin on the cytoskeleton and phenotype of chondrocytes
文章编号:
1005-202X（2020）01-0107-08
作者:
刘仁峰1; 陈诚2; 吴江怡2; 杨柳2; 宋雄波2; 黄术1; 刘宝荣1
1.湖南师范大学第一附属医院骨科医学中心， 湖南 长沙 410005； 2.第三军医大学西南医院关节外科， 重庆 400038
Author(s):
LIU Renfeng1;  CHEN Cheng2;  WU Jiangyi2;  YANG Liu2;  SONG Xiongbo2;  HUANG Shu1;  LIU Baorong1
1. Department of Orthopaedics, the First Affiliated Hospital of Hu’nan Normal University, Changsha 410005, China; 2. Department of Joint Surgery, Southwest Hospital, Army Medical University, Chongqing 400038, China
关键词:
丝切蛋白; 软骨细胞; 细胞骨架; 转染
Keywords:
Keywords: cofilin;  chondrocyte;  cytoskeleton;  transfection
分类号:
R318.04；R329
DOI:
DOI:10.3969/j.issn.1005-202X.2020.01.020
文献标志码:
A
摘要:
目的：研究丝切蛋白（cofilin）表达对软骨细胞表型及骨架的影响。方法：通过siRNA及质粒转染分别构建cofilin低表达和高表达模型，对表达不同水平cofilin的软骨细胞进行细胞骨架及相关蛋白的检测，以研究cofilin对软骨细胞表型及骨架方面的影响。结果：降低cofilin蛋白表达后，鬼笔环肽染色后观察到的软骨细胞骨架变得更为紧凑，在细胞延伸方向的肌动蛋白密度相对对照组更密集，同时软骨细胞中Ⅰ型胶原蛋白（COLⅠ）表达稍增高（137.42％[±]25.14％），结果无统计学意义（P>0.05），II型胶原蛋白（COLⅡ）表达明显降低（19.96％[±]14.59％），结果有统计学意义（P<0.05）；增加软骨细胞cofilin表达，软骨细胞骨架的免疫荧光强度较对照组稍暗，且分布稍广，COLⅠ表达降低（44.47％[±]21.67％），结果有统计学意义（P<0.05），COLⅡ表达稍增高（116.16％[±]13.18％），结果无统计学意义（P>0.05）。结论：cofilin蛋白对软骨细胞骨架具有重要的作用，cofilin蛋白的表达有助于软骨细胞维持其软骨细胞特性，cofilin蛋白表达的降低将导致软骨细胞特性的改变，这些改变在软骨损伤相关疾病的发病机制中扮演着重要的角色，将来有望通过调节这种改变来治疗软骨损伤，但其需要更进一步的研究去阐明相关机制与原理。
Abstract:
Abstract: Objective To study the effect of cofilin expression on the phenotype and skeleton of chondrocytes. Methods The low- and high-level cofilin expression models were constructed by siRNA and plasmid transfection. The cytoskeletons and related proteins in chondrocytes which had different level of cofilin expression were detected for studying the effects of cofilin on the phenotype and skeleton of chondrocytes. Results After reducing cofilin expression, the cytoskeleton of chondrocytes observed after phalloidin staining became more compact, and the actin density in the cell extension direction was denser than that of control group. Meanwhile the expression of COLⅠ in chondrocytes which was 137.42%[±]25.14% was slightly increased, without statistical significance (P>0.05), but the expression of COLⅡ which was 19.96%[±]14.59% was significantly decreased, with statistical significance (P<0.05). When increasing cofilin expression in chondrocytes, the immunofluorescence intensity of the cytoskeleton was slightly weaker than that of control group, and the distribution was slightly wider. Meanwhile the expression of COLⅠ which was 44.47%[±]21.67% was decreased, with statistical significance (P<0.05), and the expression of COLⅡ which was 116.16%[±]13.18% was slightly increased, without statistical significance (P>0.05). Conclusion Cofilin which helps chondrocytes maintain their characteristics plays an important role in the cytoskeleton of chondrocyte. The decreased expression of cofilin will lead to the change of chondrocyte characteristics, resulting in cartilage damage-related diseases. It could be a potential therapeutic method to treat cartilage damage by regulating the expression of cofilin, but further researches are required to clarify the relevant mechanisms.

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[1]黄术,杨君君,吴江怡,等. 细胞骨架结构改变对软骨细胞表型的影响[J].中国医学物理学杂志,2017,34(12):1287.[doi:DOI:10.3969/j.issn.1005-202X.2017.12.018]
　HUANG Shu,YANG Junjun,WU Jiangyi,et al. Effects of cytoskeleton changes on chondrocyte phenotype[J].Chinese Journal of Medical Physics,2017,34(1):1287.[doi:DOI:10.3969/j.issn.1005-202X.2017.12.018]
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　XU Boyang,,et al.The mechanism of histone deacetylase regulating terminal differentiation of chondrocytes[J].Chinese Journal of Medical Physics,2021,38(1):233.[doi:DOI:10.3969/j.issn.1005-202X.2021.02.019]

备注/Memo

备注/Memo:
【收稿日期】2019-06-12 【基金项目】国家自然科学基金（81572107）；湖南省科技厅重点研发计划－社发支撑领域项目（2015SK20471）；湖南师范大学临床医学院仁术科研发展基金资助项目（2015-15） 【作者简介】刘仁峰，在读硕士研究生，研究方向：骨与关节、运动医学、软骨组织再生，E-mail: 524412608@qq.com 【通信作者】刘宝荣，主任医师，研究方向：骨与关节、运动医学，E-mail: lbr9391@163.com
更新日期/Last Update: 2020-01-14