[1]王浩宇,李春舫,董霁,等.基于氢质子磁共振波谱技术研究L波段微波辐射对大鼠学习和记忆的影响[J].中国医学物理学杂志,2019,36(12):1373-1376.[doi:DOI:10.3969/j.issn.1005-202X.2019.12.002]
　WANG Haoyu,LI Chunfang,DONG Ji,et al.1H-proton magnetic resonance spectroscopy for studying effects of L-band microwave radiation on learning and memory functions of rats[J].Chinese Journal of Medical Physics,2019,36(12):1373-1376.[doi:DOI:10.3969/j.issn.1005-202X.2019.12.002]
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基于氢质子磁共振波谱技术研究L波段微波辐射对大鼠学习和记忆的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
36卷
期数:
2019年第12期
页码:
1373-1376
栏目:
医学放射物理
出版日期:
2019-12-25

文章信息/Info

Title:
1H-proton magnetic resonance spectroscopy for studying effects of L-band microwave radiation on learning and memory functions of rats
文章编号:
1005-202X（2019）12-1373-04
作者:
王浩宇1; 李春舫1; 2; 董霁1; 赵黎1; 张静1; 王惠1; 徐新萍1; 姚斌伟1; 周红梅1; 李家开3; 马林2; 彭瑞云1
1.军事科学院军事医学研究院辐射医学研究所， 北京 100850； 2.中国人民解放军总医院第一医学中心放射诊断科， 北京 100853； 3.中国人民解放军总医院海南医院放射诊断科， 海南 三亚 572013
Author(s):
WANG Haoyu1;  LI Chunfang1;  2;  DONG Ji1;  ZHAO Li1;  ZHANG Jing1;  WANG Hui1;  XU Xinping1;  YAO Binwei1;  ZHOU Hongmei1;  LI Jiakai3;  MA Lin2;  PENG Ruiyun1
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China; 2. Department of Radiodiagnosis, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; 3. Department of Radiodiagnosis, Hainan Hospital of PLA General Hospital, Sanya 572013, China
关键词:
大鼠; 微波辐射; 氢质子磁共振波谱; 神经元; 学习记忆
Keywords:
Keywords: rat;  microwave radiation;  1H-proton magnetic resonance spectroscopy;  neuron;  learning and memory
分类号:
R318；R811.1
DOI:
DOI:10.3969/j.issn.1005-202X.2019.12.002
文献标志码:
A
摘要:
目的：基于氢质子磁共振波谱（1H-MRS）技术，分析L波段微波辐射对大鼠学习和记忆功能的影响。方法：SPF级雄性Wistar大鼠26只，随机分为假辐射组（Sham组）、L波段30 mW/cm2微波辐射组（L30组）。将L30组大鼠放置于有机玻璃照射盒中，使用L波段微波辐射源从背侧照射10 min；Sham组大鼠除未行微波辐射外其他处理与照射组相同。采用Morris水迷宫方法于辐射后1 d和7 d对各组大鼠的学习和记忆功能进行检测。使用1H-MRS于辐射后1 d和7 d检测鼠脑中的N-乙酰天门冬氨酸（N-acetylaspartate, NAA）、胆碱（Choline, Cho）、肌酸（Creatine, Cr）水平。结果：微波辐射后1 d和7 d，与Sham组相比，L30组平均逃避潜伏期有延长趋势，但无统计学意义（P>0.05）。1H-MRS检测结果显示辐射后1 d，与Sham组比较，L30组1H-MRS波谱NAA/Cr值显著降低（P<0.05），Cho/Cr值无显著差异（P>0.05）；辐射后7 d，与Sham组比较，L30组大鼠脑部NAA/Cr和Cho/Cr值均无显著差异（P>0.05）。结论：30 mW/cm2 L波段微波辐射后1 d大鼠脑内NAA/Cr显著降低，而学习和记忆行为学指标无显著改变，提示利用1H-MRS技术能够在被试未出现显著行为学改变时检测出微波辐射对学习和记忆相关神经元的影响。
Abstract:
Abstract: Objective To investigate the effects of L-band microwave radiation on the learning and memory functions of rats based on 1H-proton magnetic resonance spectroscopy (1H-MRS). Methods Twenty-six SPF male Wistar rats were randomly divided into Sham group and 30 mW/cm2 L-band microwave radiation group (L30 group). The rats in L30 group were restrained in plastic cages and exposure to 30 mW/cm2 L-band microwave radiation for 10 min. The incident direction of the microwave was from dorsal to ventral. The rats in Sham group were also restrained in the same cages for 10 min, without any microwave radiation. Both learning and memory functions of rats were evaluated at 1 d and 7 d after the radiation using Morris water maze. The level of N-acetylaspartate (NAA), choline (Cho) and creatine (Cr) in the rats’ brain were measured with 1H-MRS. Results Compared with that of Sham group, the average escaping latency of L30 group at 1 d and 7 d after microwave radiation was prolonged, but there was no statistical significance between two groups (P>0.05). The results of 1H-MRS examination demonstrated that at 1 d after the radiation, the NAA/Cr of L30 group was significantly lower than that of Sham group (P<0.05), while the Cho/Cr was similar in two groups, without significant differences (P>0.05); and that at 7 d after the radiation, there was no significant difference in both of NAA/Cr and Cho/Cr between L30 group and Sham group (P>0.05). Conclusion 30 mW/cm2 L-band microwave radiation can lead to a significant decrease of intracerebral NAA/Cr in rat at 1 d after microwave exposure, without resulting in obvious changes in the behavioral indexes of learning and memory, which suggests that 1H-MRS can be used for evaluating the microwave radiation induced damage to neurons related to learning and memory before any significant changes of behavioral measurements can be observed.

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备注/Memo

备注/Memo:
【收稿日期】2018-12-12 【基金项目】国家自然科学基金青年基金（61801506）；海南省自然科学基金（817354） 【作者简介】王浩宇，博士，助理研究员，研究方向：电磁辐射生物效应，E-mail: semart106@163.com；李春舫，硕士研究生，技师，研究方向：磁共振成像技术及其在微波辐射损伤研究中的应用，E-mail: lichunfang5@163.com（王浩宇与李春舫为共同第一作者） 【通信作者】彭瑞云，博士，研究员，研究方向：电磁辐射生物效应，E-mail: pengry@bmi.ac.cn
更新日期/Last Update: 2019-12-24