[1]赵一蔚,任培乐,于凤旭,等. 剪切应力强度对内皮细胞Piezo1表达的影响[J].中国医学物理学杂志,2019,36(4):474-478.[doi:DOI:10.3969/j.issn.1005-202X.2019.04.020]
　ZHAO Yiwei,REN Peile,YU Fengxu,et al. Effects of shear stress intensity on the expression of Piezo1 in vascular endothelial cells[J].Chinese Journal of Medical Physics,2019,36(4):474-478.[doi:DOI:10.3969/j.issn.1005-202X.2019.04.020]
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 剪切应力强度对内皮细胞Piezo1表达的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
36卷
期数:
2019年第4期
页码:
474-478
栏目:
医学生物物理
出版日期:
2019-04-25

文章信息/Info

Title:
 Effects of shear stress intensity on the expression of Piezo1 in vascular endothelial cells
文章编号:
1005-202X（2019）04-0474-05
作者:
 赵一蔚1; 任培乐1; 于凤旭2; 代红燕3; 聂永梅2
 1.新疆医科大学基础医学院生理学教研室， 新疆 乌鲁木齐 830011； 2.西南医科大学附属医院心脏大血管外科， 四川 泸州 646000； 3.新疆医科大学基础医学院机能中心实验室， 新疆 乌鲁木齐 830011
Author(s):
 ZHAO Yiwei1;  REN Peile1;  YU Fengxu2;  DAI Hongyan3;  NIE Yongmei2
 1. Department of Physiology, Collage of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China; 2. Department of Cardiovascular Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 3. Laboratory Centre of Mechanism, Collage of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China
关键词:
 ; 剪切应力; Piezo1; 内皮细胞; 动脉粥样硬
Keywords:
 ; Keywords: shear stress;  Piezo1;  endothelial cell;  arteriosclerosis
分类号:
R35
DOI:
DOI:10.3969/j.issn.1005-202X.2019.04.020
文献标志码:
A
摘要:
 目的：探究不同强度（0、2.37、4.14、7.11、9.47、14.21、17.76 dyne/cm2）剪切应力对血管内皮细胞Piezo1表达的影响。方法：选取人脐静脉内皮细胞株EA.hy926进行常规体外培养，使用平行板流槽加载不同强度剪切应力，分别采用实时荧光定量PCR及Western-blot方法从基因水平和蛋白水平检测Piezo1表达情况。结果：剪切应力强度影响Piezo1的表达，加载剪切应力为4.14 dyne/cm2时，Piezo1 mRNA和蛋白表达与静止状态相比均显著减少（P<0.05）。结论：剪切应力影响内皮细胞中Piezo1改变，且强度为4.14 dyne/cm2时最为明显，提示Piezo1可能参与低剪切应力诱导的动脉粥样硬化过程。
Abstract:
 Abstract: Objective To investigate the effects of shear stress of different intensities (0, 2.37, 4.14, 7.11, 9.47, 14.21, 17.76 dyne/cm2) on the expression of Piezo1 in vascular endothelial cells. Methods The human umbilical vein endothelial cell lines (EA.hy926) were cultured in vitro, and the shear stress of different intensities were applied on endothelial cell lines by parallel-plate flow chamber. Real-time fluorescent quantitative PCR and Western-blot analysis were used to detect the expression of Piezo1 in endothelial cell lines at gene level and protein level. Results The expression of Piezo1 was affected by shear stress intensity. The expressions of Piezo1 mRNA and proteins under a shear stress of 4.14 dyne/cm2 were significantly reduced as compared with those in control group (P<0.05). Conclusion Shear stress intensity has significant effects on the expression of Piezo1 in vascular endothelial cells, and when the shear stress intensity is 4.14 dyne/cm2, the effect is the most obvious, which indicates that Piezo1 may involve in the arteriosclerosis induced by shear stress of low intensity.

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备注/Memo

备注/Memo:
 【收稿日期】2018-11-23
【基金项目】新疆维吾尔自治区自然科学基金联合基金项目（2018D0-1C161）；泸州市人民政府-西南医科大学科技战略合作项目（2018LZXNYD-ZK27）
【作者简介】赵一蔚，硕士，研究方向：心血管力学生物学，E-mail: zhaoyw126@126.com
【通信作者】聂永梅，教授，研究方向：心血管力学生物学，E-mail: nieyongmei@126.com
更新日期/Last Update: 2019-04-23