[1]张克英,陈启亮,芮刚,等. 大气压冷等离子体对大鼠凝血功能的影响[J].中国医学物理学杂志,2019,36(3):351-355.[doi:DOI:10.3969/j.issn.1005-202X.2019.03.021]
　ZHANG Keying,CHEN Qiliang,et al. Effects of cold atmospheric plasma on coagulation function of rats[J].Chinese Journal of Medical Physics,2019,36(3):351-355.[doi:DOI:10.3969/j.issn.1005-202X.2019.03.021]
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 大气压冷等离子体对大鼠凝血功能的影响()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
36卷
期数:
2019年第3期
页码:
351-355
栏目:
医学生物物理
出版日期:
2019-03-25

文章信息/Info

Title:
 Effects of cold atmospheric plasma on coagulation function of rats
文章编号:
1005-202X（2019）03-0351-05
作者:
 张克英1; 6; 陈启亮2; 芮刚1; 6; 武霄铮1; 3; 郭玲1; 6; 张俊平1; 6; 刘志强4; 5; 李和平5; 丁桂荣1; 6
 1.空军军医大学军事预防医学系辐射防护医学教研室， 陕西 西安 710032； 2.空军军医大学基础医学院三大队二中队， 陕西 西安 710032； 3.中国人民解放军78506部队， 四川 成都 610000； 4.华北理工大学机械工程学院， 河北 唐山 063210； 5.清华大学工程物理系， 北京100084； 6.特殊作业环境危害评估与防治教育部重点实验室， 陕西 西安 710032
Author(s):
 ZHANG Keying1;  6;  CHEN Qiliang2;  RUI Gang1;  6;  WU Xiaozheng1;  6;  GUO Ling1;  6;  ZHANG Junping1;  6;  LIU Zhiqiang4;  5;  LI Heping5;  DING Guirong1;  6
 1. Department of Medical Radiation Protection, Faculty of Military Preventive Medicine, Air Force Medical University, Xi’an 710032, China; 2. The Second Squadron of Basic Medical School, Air Force Medical University, Xi’an 710032, China; 3. the 78506th Troops of Chinese People’s Liberation Army, Chengdu 610000, China; 4. College of Mechanical Engineering, North China University of Science and Technology, Tangshan 063210, China; 5. Department of Engineering Physics, Tsinghua University, Beijing 100084, China; 6.Key Laboratory of Hazard Assessment and Control in Special Operational Environment, Ministry of Education, Xi’an 710032, China
关键词:
 ; 大气压冷等离子体; 血滴凝固; 出血模型; 大鼠
Keywords:
 ; Keywords: cold atmospheric plasma;  blood coagulation;  bleeding model;  rat
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2019.03.021
文献标志码:
A
摘要:
 目的：筛选和探讨大气压冷等离子体（CAP）对血液凝固的影响并筛选其最佳参数，初步探讨其作用机制。方法：首先，利用不同作用距离（1、2、3 cm），不同放电电压（3.33、6.73、8.03 kV），不同作用时间（30、60、120 s）的CAP处理大鼠血滴，观察不同参数CAP对血滴凝固效果的影响，筛选CAP促进凝血的最佳参数。之后，将22只SD大鼠随机分为空白对照组（n=11）和CAP处理组（n=11），验证上述最佳CAP参数对SD大鼠出血模型的止血效果。最后，通过抗凝血滴实验探讨CAP促进血液凝固的机制。结果：体外血滴实验结果显示，与对照组相比，CAP组血滴凝固效果更明显，主要表现为血滴表面干燥和酒窝样凹陷增多，且血滴凝固效果与CAP处理时间和放电电压呈正相关，与CAP作用距离呈负相关。动物出血模型研究结果显示，放电电压8.03 kV、作用时间120 s、作用距离1 cm的CAP处理出血部位可以显著缩短出血时间并降低出血量。抗凝血滴结果显示，CAP可以显著促进枸橼酸钠抗凝血滴的凝固。结论：CAP对血液凝固具有明显的时间促进作用，且与作用距离和放电电压密切相关。其机制可能与CAP促进纤维蛋白原向纤维蛋白转化有关。
Abstract:
 Abstract: Objective To investigate the effects of cold atmospheric plasma (CAP) on blood coagulation, determine the optimal parameters and explore its mechanism. Methods Firstly, the blood drops of Sprague-Dawley (SD) rats were treated with CAP with different treatment distances (1, 2 and 3 cm), different discharge voltages (3.33, 6.73 and 8.03 kV), and different treatment durations (30, 60 and 120 s) to investigate the effects of CAP with different parameters on blood coagulation and determine the optimal parameters for blood coagulation. Subsequently, 22 SD rats were randomly divided into control group (n=11) and CAP treatment group (n=11) to verify the hemostatic effect of CAP with the optimal parameters in different rat bleeding models. Finally, the mechanism of CAP to promote blood coagulation was explored by anticoagulation test. Results The results of in vitro blood test showed that CAP significantly promoted blood coagulation, which was featured with surface drying and the increasing number of dimple signs. Besides, the effect of CAP on blood coagulation was positively correlated with treatment durations and discharge voltages, but negatively correlated with treatment distances. The research on rats bleeding models showed that CAP with the optimal parameters (discharge voltage of 8.03 kV, treatment duration of 120 s, and treatment distance of 1 cm) significantly shortened the bleeding time and reduced the amount of bleeding. The results of anticoagulant test proved that CAP could significantly promote the coagulation of anticoagulant blood drops with sodium citrate. Conclusion CAP significantly shortens the time for blood coagulation. The effect of CAP on blood coagulation is closely dependent on treatment distance, discharge voltage and treatment duration, and its mechanism may be related with the conversion of fibrinogen to fibrin.

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备注/Memo

备注/Memo:
 【收稿日期】2018-12-05
【基金项目】国家自然科学基金（31770905，11475103）；陕西省社会发展科技攻关项目（2016SF-018）
【作者简介】张克英，硕士研究生，研究方向：辐射与再生医学，E-mail: zhangky@fmmu.edu.cn
【通信作者】丁桂荣，博士生导师，研究方向：生物电磁学，E-mail: dingzhao@fmmu.edu.cn；李和平，博士生导师，研究方向：等离子体科学与技术，E-mail: liheping@tsinghua.edu.cn
更新日期/Last Update: 2019-03-26