糖尿病对去势大鼠骨生物力学的影响-《中国医学物理学杂志》

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Title:: Effects of diabetes on bone biomechanics in ovariectomized rats

文章编号:: 1005-202X（2018）09-1099-05

作者:: 解琪琪1; 2; 3; 李文洲1; 2; 3; 邓亚军1; 2; 3; 史卫东1; 2; 3; 任恩惠1; 2; 3; 马靖琳2; 潘云燕1; 康学文1; 3; 汪静; . 兰州大学第二医院骨科，甘肃兰州730000；2. 甘肃省骨关节疾病研究重点实验室，甘肃兰州730000；3. 兰州大学，甘肃兰州730000

Author(s):: XIE Qiqi1; 2; 3; LIWenzhou1; 2; 3; DENG Yajun1; 2; 3; SHIWeidong1; 2; 3; REN Enhui1; 2; 3; MAJinglin2; PAN Yunyan1; KANG Xuewen1; 3; WANG Jing1; 2; 3; 1. Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou 730000, China; 2. Key Laboratory of Osteoarticular Disease of Gansu Province, Lanzhou 730000, China; 3. Lanzhou University, Lanzhou 730000, China

关键词:: I型糖尿病; 去势大鼠; 骨质疏松; 骨密度; 生物力学

Keywords:: Keywords: type I diabetes; ovariectomized rat; osteoporosis; bone mineral density; biomechanics

分类号:: R318.01

DOI:: 10.3969/j.issn.1005-202X.2018.09.019

文献标志码:: A

摘要:: 目的：探讨I型糖尿病对去势大鼠骨组织的骨密度及生物力学参数的影响。方法：选取SPF级雌性3月龄SD大鼠40只，采用随机数字表法分成4组：假手术组（Sham组）、单纯去势组（A组）、糖尿病组（B组）、糖尿病合并去势组（C组），每组10只，A组进行双侧卵巢切除法制作骨质疏松大鼠模型，B组采用链脲佐菌素腹腔注射法制作糖尿病大鼠模型，C组进行手术摘除卵巢7 d后腹腔注射链脲佐菌素制作糖尿病合并去势大鼠模型，成模后继续饲养3个月，处死大鼠，收集腰椎及股骨依次行双能X线检查、生物力学检测。结果：A、B、C组较Sham组骨密度下降明显，差异具有统计学意义（P< 0.05），其中C组各感兴趣部位下降最为明显，B组其次，A组下降相对不显著。A组下降部位以腰椎（全段、L6、L4、L3）、整体股骨及其远心端和近心端较为明显，B组下降部位则是以腰椎（全段、L3、L2、L1）及股骨的远心端和近心端明显，而C组各感兴趣区下降均显著，以腰椎（全段、L1、L2、L3、L4）及股骨远心端和近心端明显，L6则相对下降不显著。A、B、C组较Sham 组在股骨三点弯曲实验、腰椎压缩实验所得生物力学等指标显著下降，且趋势与骨密度变化趋势一致，C组下降最为显著。结论：I型糖尿病加剧去势大鼠骨密度及生物力学指标的下降，但骨丢失敏感部位与单纯去势大鼠部位却不尽相同，去势大鼠以腰椎（全段、L6）及股骨远心端、近心端为骨丢失的最敏感区域，单纯I型糖尿病大鼠则则以腰椎（全段、L3、L2、 L1）及股骨的远心端和近心端为骨丢失敏感区域，而I型糖尿病合并去势大鼠是腰椎（L1、L2、L3、L4、全段）及股骨远心端和近心端为骨丢失敏感区域。

Abstract:: Abstract: Objective To investigate the effects of type I diabetes on bone mineral density (BMD) and biomechanical parameters in ovariectomized rats. Methods Forty female Sprague-Dawley rats aged 3 months of SPF were randomly divided into 4 groups, namely sham operation group (Sham group), simple ovariectomized group (group A), diabetes group (group B), and diabetes combined with ovariectomized group (group C), with 10 rats in each group. The rats in group A were subjected to bilateral oophorectomy to establish osteoporotic rat models, and the rats in group B were treated with intraperitoneal injection of streptozotocin (STZ) to establish diabetic rat models. After 7 days of surgical removal of the ovary, the rats in group C were intraperitoneally injected with STZ and underwent lumbar compression test and femoral three-point bend test to prepare diabetes combined with ovariectomized models. The rats were kept for 3 months after modeling. Then the rats were sacrificed to collect lumbar vertebrae and femoral bone for dual-energy X-ray examination and biomechanical examination. Results The BMD in group A, B and C was significantly lower than that in Sham group, with statistical significance (P<0.05), and the most significant BMD decrease in regions of interest was observed in group C, followed by group B and then group A. In group A, the BMD decreases at lumbar spine (the whole segment, L6, L4, L3), the whole femur and its distal and proximal ends were the most obvious. In group B, the BMD decreases were mainly found at the lumbar vertebra (the whole segment, L3, L2, L1) femurs, and the distal and proximal ends of the femur. In group C, the BMD at all regions of interest were significantly decreased, and the decreases at the lumbar vertebrae (the whole segment, L1, L2, L3, L4), and the distal and proximal ends of the femur were relatively obvious when compared with the BMD decrease at the L6. Compared with Sham group, group A, B and C showed significant decreases in the biomechanical parameters obtained with femoral three-point bending test and lumbar compression test, with a downtrend consistent with the change trend of BMD, and the decline in Group C was the most significant. Conclusion Type I diabetes aggravates the decrease of BMD and biomechanics in ovariectomized rats, but the site of bone loss sensitivity is not the same as that of ovariectomized rats. For ovariectomized rats, the most sensitive areas of bone loss were the lumbar vertebrae (the whole segment, L6), and the distal and proximal ends of the femur; for diabetes rats, those were lumbar vertebrae (the whole segment, L3, L2, L1), and the distal and proximal ends of the femur; and for diabetes combined with ovariectomized rats, those were lumbar vertebrae (L1, L2, L3, L4, the whole segments), distal and proximal ends of the femur.

《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

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