[1]丁宁,闫亮,万浪,等. NFATc1-ERK5通路介导流体剪切力促进成骨细胞BMP7表达[J].中国医学物理学杂志,2018,35(3):358-363.[doi:DOI:10.3969/j.issn.1005-202X.2018.03.020]
　DING Ning,YAN Liang,et al. Increased expression of BMP7 by fluid shear stress mediated through NFATc1-ERK5 pathway[J].Chinese Journal of Medical Physics,2018,35(3):358-363.[doi:DOI:10.3969/j.issn.1005-202X.2018.03.020]
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 NFATc1-ERK5通路介导流体剪切力促进成骨细胞BMP7表达()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
35卷
期数:
2018年第3期
页码:
358-363
栏目:
医学生物物理
出版日期:
2018-03-20

文章信息/Info

Title:
 Increased expression of BMP7 by fluid shear stress mediated through NFATc1-ERK5 pathway
文章编号:
1005-202X（2018）03-0358-06
作者:
 丁宁1; 2; 闫亮1; 2; 万浪1; 2; 夏亚一1; 2
 1.兰州大学第二医院骨科研究所， 甘肃 兰州 730000； 2.甘肃省骨关节疾病研究重点实验室， 甘肃 兰州730000
Author(s):
 DING Ning1;  2;  YAN Liang1;  2;  WAN Lang1;  2;  XIA Yayi1;  2
 1. Institute of Orthopedics, Lanzhou University Second Hospital, Lanzhou 730000, China; 2. Key Laboratory for Orthopedics of Gansu Province, Lanzhou 730000, China
关键词:
 ; NFATc1; ERK5; BMP7; 流体剪切力; 成骨细胞
Keywords:
 ; nuclear factor of activated T cells c1;  extracellular-regulated protein kinase 5;  bone morphogenetic protein 7;  fluid shear stress;  osteoblast
分类号:
R35
DOI:
DOI:10.3969/j.issn.1005-202X.2018.03.020
文献标志码:
A
摘要:
 目的：探讨成骨细胞中NFATc1与ERK5调控关系，并研究流体剪切力（FSS）调节BMP7表达的信号通路。 方法：实验分为6组，即空白组、FSS组、CsA（Cyclosporin, NFATc1抑制剂）组、XMD8-92（ERK5抑制剂）组、FSS+CsA组、FSS+XMD8-92组。用Western Blot方法检测不同干预条件下NFATc1、ERK5、p-ERK5以及BMP7蛋白表达水平并进行比较。 结果：12 dyn/cm2 FSS作用45 min能够显著提高NFATc1、p-ERK5在成骨细胞内水平，400 nmol/L CsA干预30 min能够有效抑制NFATc1表达量升高以及ERK5磷酸化，但5 μmol/L XMD8-92作用1 h仅能够抑制ERK5磷酸化，而对NFATc1没有作用。此外，FSS促进MC3T3-E1细胞中BMP7表达量升高，CsA和XMD8-92任何一种抑制剂均能显著阻止BMP7表达。 结论：FSS在成骨细胞中通过NFATc1来调控ERK5磷酸化，BMP7为ERK5下游靶点受NFATc1-ERK5通路调控。
Abstract:
 Objective To discuss the interrelation between nuclear factor of activated T cells c1 (NFATc1) and extracellular-regulated protein kinase 5 (ERK5), and explore the fluid shear stress (FSS)-induced signal pathway that regulates the expression of bone morphogenetic protein 7 (BMP7). Methods The experiment was divided into 6 groups, namely control group, FSS group, CsA (Cyclosporin, NFATc1 inhibitor) group, XMD8-92 (ERK5 inhibitor) group, FSS+CsA group, and FSS+XMD8-92 group. Western Blot was applied to access the expression level of NFATc1, ERK5, p-ERK5 and BMP7. Results 12 dyn/cm2 FSS for 45 min well promoted the level of NFATc1 and p-ERK5 in osteoblasts. 400 nmol/L CsA for 30 min effectively inhibited the expression of NFATc1 and the phosphorylation of ERK5, but 5 μmol/L XMD8-92 for 1 h only blocked the phosphorylation of ERK5, without causing effects on NFATc1. The expression level of BMP7 in MC3T3-E1 cell was promoted by FSS, and either CsA or XMD8-92 significantly inhibited the expression of BMP7. Conclusion FSS mediates the phosphorylation of ERK5 in osteoblasts by NFATc1, and BMP7 regarded as downstream target of ERK5 was regulated by NFATc1-ERK5 pathway.

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备注/Memo

备注/Memo:
 【收稿日期】2017-12-02
【基金项目】国家自然科学基金（81672207）；甘肃省自然科学基金（817JR5RA188）；兰州市科技发展计划项目（2017-4-88）
【作者简介】丁宁，硕士，E-mail: dingninghr@yeah.net
【通信作者】夏亚一，E-mail: xiayyldey@126.com
更新日期/Last Update: 2018-03-21