[1]黄术,吴江怡,杨君君,等. 天冬氨酸修饰纳米羟基磷灰石/聚乳酸复合物制备软骨组织工程支架及其生物性能研究[J].中国医学物理学杂志,2018,35(5):616-620.[doi:DOI:10.3969/j.issn.1005-202X.2018.05.022]
　HUANG Shu,WU Jiangyi,YANG Junjun,et al.Preparation of bone tissue engineering scaffold with aspartic acid-modified nano-hydroxyapatite/poly L-lactic acid and its bio-characterizations[J].Chinese Journal of Medical Physics,2018,35(5):616-620.[doi:DOI:10.3969/j.issn.1005-202X.2018.05.022]
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 天冬氨酸修饰纳米羟基磷灰石/聚乳酸复合物制备软骨组织工程支架及其生物性能研究()
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]

卷:
35卷
期数:
2018年第5期
页码:
616-620
栏目:
生物材料与力学
出版日期:
2018-05-22

文章信息/Info

Title:
Preparation of bone tissue engineering scaffold with aspartic acid-modified nano-hydroxyapatite/poly L-lactic acid and its bio-characterizations
文章编号:
1005-202X（2018）05-0616-05
作者:
 黄术; 吴江怡; 杨君君; 周益昭; 刘宝荣; 夏铎; 刘仁峰
 湖南师范大学第一附属医院（湖南省人民医院）骨科医学中心， 湖南 长沙 410005
Author(s):
 HUANG Shu;  WU Jiangyi;  YANG Junjun;  ZHOU Yizhao;  LIU Baorong;  XIA Duo;  LIU Renfeng
 Department of Orthopedics, Hunan Provincial People’s Hospital (the First Affiliated Hospital of Hunan Normal University), Changsha 410005, China
关键词:
 ; 纳米羟基磷灰石; 左旋聚乳酸; 天冬氨酸; 复合材料; 生物相容性
Keywords:
 ; Keywords: nano-hydroxyapatite;  poly L-lactic acid;  aspartic acid;  composite;  biocompatibility
分类号:
R318
DOI:
DOI:10.3969/j.issn.1005-202X.2018.05.022
文献标志码:
A
摘要:
目的：通过天冬氨酸修饰的纳米羟基磷灰石（Asp-nHA）和左旋聚乳酸（PLLA）复合，制备软骨组织工程支架，并进行体外实验研究，探索其作为软骨组织工程支架的可行性。 方法：以碳二亚胺盐酸盐/N-羟基琥珀酰亚胺偶联法制备Asp-nHA，并与PLLA复合，获得新型Asp-nHA/PLLA纳米复合支架。比较成骨细胞在该新型材料表面的粘附、生长和增殖的情况。 结果：加入nHA后，Asp-nHA/PLLA复合材料可显著增强细胞的粘附、生长、增殖；Asp-nHA/PLLA的生物学性能明显优于PLLA及HA/PLLA，且Asp-nHA/PLLA具有最强的体外诱导成骨细胞分化能力。 结论：Asp-nHA/PLLA制备简单，具有良好的生物相容性和成骨活性，是一种性能良好的骨组织工程支架材料。
Abstract:
 Abstract: Objective To design and prepare surface modified cartilage tissue scaffolds with the use of aspartic acid-modified nano-hydroxyapatite (Asp-nHA) and poly L-lactic acid (PLLA), and evaluate its feasibility as a scaffold for cartilage tissue engineering through in vitro experiment. Methods Asp-nHA was synthesized with the standard EDC/NHS coupling method, and the resultant Asp-nHA was conjugated to PLLA to form Asp-nHA/PLLA composite scaffold. The osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were evaluated. Results Osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were significantly increased due to the addition of nHA. The in vitro analysis illustrated that Asp-nHA/PLLA composite was superior to PLLA and HA/PLLA in bio-characterizations and had the strongest ability to induce osteogenic differentiation in vitro. Conclusion Asp-nHA/PLLA is simple to be prepared and its good biocompatibility and osteogenic activity make it become a good kind of scaffold for cartilage tissue engineering.

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备注/Memo

备注/Memo:
 【收稿日期】2017-11-14
【基金项目】长沙市科技计划项目基础研究项目（kq1701058）；重庆市博士后科研项目特别资助项目（Xm2016052）；湖南师范大学临床医学院仁术科研发展基金资助项目（2015-15）；湖南省科技厅社会发展支撑计划项目（2015SK20471）
【作者简介】黄术，博士，主治医师，研究方向：膝关节镜微创治疗，髋、膝人工关节置换，膝关节运动损伤基础及临床研究，E-mail: 9258024@qq.com
【通信作者】刘宝荣，教授，主任医师，研究方向：髋膝关节置换术、关节镜微创技术及肩关节外科等各类骨科疾病的治疗，E-mail: lbr9391@163.com
更新日期/Last Update: 2018-05-22