In vivo visualization of tumor microenvironment based on mouse dorsal skin-fold window chamber(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第9期
- Page:
- 1077-1083
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- In vivo visualization of tumor microenvironment based on mouse dorsal skin-fold window chamber
- Author(s):
- JIANG Yihang1; XIAO Huiyu1; 2; CAO Zhonglin1; 3; ZHAI Peng4; XU Zhourui1; LI Li5; ZOU Xiaomin1; MA Mingze1; WANG Xiaomei5;
LIN Guimiao5; XU Gaixia1
- 1. School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518055, China 2. Shenzhen Institute for
Drug Control, Shenzhen 518057, China 3. School of Materials Science and Engineering, Guizhou Minzu University, Guiyang 550025,
China 4. Health Science Center, Shenzhen University, Shenzhen 518055, China 5. Department of Physiology, School of Basic Medical
Sciences, Health Science Center, Shenzhen University, Shenzhen 518055, China
- Keywords:
- tumor microenvironment dorsal skin-fold window chamber in vivo imaging nano-gene therapy medication
evaluation
- PACS:
- R318；R73-37
- DOI:
- 10.3969/j.issn.1005-202X.2021.09.006
- Abstract:
- Objective To construct a mouse dorsal skin-fold window chamber (DSFC) model for monitoring the tumor
microenvironment continuously, researching tumor proliferation and spread, and providing a technical method to evaluate the
efficacy of anti-tumor drugs. Methods The mouse 4T1 breast cancer cells transfected with green fluorescent protein were used
to construct a mouse DSFC tumor model for the real-time dynamic and continuous monitoring of tumor size, vascular deformation,
blood perfusion and other parameters in the tumor microenvironment, thereby obtaining the precise tumor microenvironment
information. Meanwhile, the proposed model was also used for evaluating the therapeutic effect of PCL-PDEM-siRNAin nanogene
therapy. Results The tumorigenesis rate of 4T1 breast cancer cells inoculated in the DSFC model of nude mice was higher
than 90%. Two days after tumor inoculation, the capillaries in the inoculated area were increased. On the 4th day after tumor
inoculation, the tumor morphology with clear boundaries could be observed, and the blood perfusion volume at the tumor area
was increased significantly on the 4th to 8th days after tumor inoculation. Four days after nanomedicine injection, the growth rate
of the tumor area slowed down, and a significant tumor suppressor effect appeared and 8 days after nanomedicine injection, the
neovascularization inside the tumor was decreased, and the blood perfusion volume was beginning to stabilize. Conclusion The
study provides a new strategy for the refined imaging of the tumor microenvironment, and is of great significance to the basic
research of tumor biology and the development of anti-tumor drugs.
Last Update: 2021-09-27