Abstract

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Title:: Fluorescence image analysis technique for digital isothermal amplification system

Author(s):: XU Jing1; WANG Lei2; ZHAO Jun2; HUA Changyi2; ZHU Ling2; WANG Minglong2; ZHOU Zhengxian1; 1. Institute of Applied Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences/Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument/Anhui Provincial Engineering Laboratory for Medical Optical Diagnosis and Treatment Technology and Instrument, Hefei 230031, China 2. School of Physics and Electronic Information, Anhui Normal University, Wuhu 241002, China

Keywords:: Keywords: digital isothermal amplification technology fluorescence image microfluidic chip Image J image processing software

Abstract:: The digital isothermal amplification technology based on microfluidic chips, as a new molecular detection technology, can be used to specifically process the collected fluorescence image, thereby achieving the absolute quantitative analysis on the target to be tested. A supporting software analysis system is developed with the fluorescence images collected by the self-developed digital microfluidic chips as the analysis object and based on the consideration of the actual application scenario. The florescence image processing part of the developed system has such functions as image splicing, image tilt correction, intercept of target area, image segmentation and fluorescence spot counting. This analysis system can satisfy the requirement of nucleic acid molecular number detection and has good human-machine interaction performance. The analysis results obtained by the system are consistent with the statistical results of Image J image processing software, and moreover the proposed system can achieve an automatic calculation and analysis of the detected results, which lays a solid foundation for the wider application of the analysis system in the future.