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Title:: Investigation into the effects of high glucose and high fat on endothelial cell Youngs modulus using atomic force microscope

Author(s):: XIONG Yuxuan1; SU Xiangjiang2; YANG An1; SU Aohan1; LONG Mengqiu4; WEN Doudou3; YANG Kaiwei4; 1. Xiangya School of Medicine, Central South University, Changsha 410013, China 2. Department of Oncology, the Central Hospital of Shaoyang, Shaoyang 422000, China 3. School of Life Sciences, Central South University, Changsha 410013, China 4. School of Physics, Central South University, Changsha 410012, China

Keywords:: atherosclerosis endothelial cell Youngs modulus atomic force microscope high glucose high fat

Abstract:: The Youngs modulus of human umbilical vein endothelial cells (HUVEC) cultured under high-glucose or high-fat conditions was measured using atomic force microscope and compared to discuss the effects of these two treatments on HUVEC. The results demonstrate that the Youngs modulus of HUVEC differs significantly following high-glucose or high-fat treatment. The Youngs modulus was (3.33±0.25)×104 Pa (n=10) in high-fat-cultured HUVEC and (2.69±0.17)×104 Pa (n=21) in high-glucose-cultured cells, with the former being 23.8% higher than the latter (P<0.001), suggesting that the two conditions may induce endothelial cell injury through divergent mechanisms. Therefore, investigating the injury of endothelial cells cultured in vitro from a biomechanical perspective can provide insights into the pathological and biomechanical alterations underlying atherosclerosis.