Mechanism of pulsed electromagnetic field in inhibiting inflammatory response in knee osteoarthritis rats through Wntβ-catenin signaling pathway(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2022年第5期
- Page:
- 617-622
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Mechanism of pulsed electromagnetic field in inhibiting inflammatory response in knee osteoarthritis rats through Wntβ-catenin signaling pathway
- Author(s):
- BAI Zhongxi; FANG Xinggang; MA Longxiang; HE Yulin
- Department of Hydrotherapy and Physiotherapy, Rehabilitation Center, Shiyan Taihe Hospital (Affiliated Hospital of Hubei University of Medicine), Shiyan 442000, China
- Keywords:
- Keywords: knee osteoarthritis pulsed electromagnetic field Wntβ-catenin signal inflammatory response
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2022.05.016
- Abstract:
- Abstract: Objective To explore the mechanism of pulsed electromagnetic field in reducing inflammatory response in knee osteoarthritis rats through the signal transduction mechanism of Wntβ-catenin. Methods A total of 90 SD rats were selected and divided into normal group, model group and pulsed electromagnetic field group, with 30 rats each. Except for normal group, the rat model of knee osteoarthritis was constructed in the other two groups. The local skin temperature, knee circumference and Lequesne MG score were measured in each group. Toluidine blue staining was used for Mankins scoring, Western Blot for obtaining the expression levels of apoptosis-regulating proteins Caspase-3 and Caspase-8, and ELISA for determining the expression levels of IL-1β, MMP-13 and TNF-α in synovial membrane. Moreover, the differences in mRNA and protein expressions of Wnt and β-catenin were analyzed, and the mitochondrial membrane potential was also measured. Results Compared with those in normal group, the local skin temperature, knee circumference, Lequesne MG score, Mankins score, expression levels of Wntβ-catenin, synovial IL-1β, MMP-13 and TNF-α in model group were significantly up-regulated (P<0.05). The knee circumference, Lequesne MG score, Mankins score, expression levels of Wntβ-catenin, synovial IL-1β, MMP-13 and TNF-α in pulsed electromagnetic field group were all significantly down-regulated as compared with model group (P<0.05). Compared with that in normal group, the mitochondrial membrane potential of articular cartilage in the other two groups was decreased, and the mitochondrial membrane potential of pulsed electromagnetic field group was lower than that of model group. Conclusion Pulse electromagnetic field can reduce the inflammatory response in knee osteoarthritis model rats, repair cartilage injury effectively, down-regulate the expression of inflammatory factors and inhibit Wntβ-catenin signal transduction.
Last Update: 2022-05-27