Detection of the protective effect of ligustrazine against oxidative stress damages to retinal pigment epithelium cell via Raman spectroscopy(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第7期
- Page:
- 864-870
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Detection of the protective effect of ligustrazine against oxidative stress damages to retinal pigment epithelium cell via Raman spectroscopy
- Author(s):
- XIE Ting1; CHEN Yang2; CHEN Wenyi1; LAI Qiaoling1; CAO Gaigai2; XU Yunchao3; HUANG Yan1
- 1. Department of Ophthalmic Optics, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China 2. Department of Medical Laboratory Science, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China 3. Key Laboratory of Medical Optoelectronic Science and Technology, Ministry of Education, Fujian Normal University, Fuzhou 350004, China
- Keywords:
- Keywords: retinal pigment epithelium cell Raman spectroscopy reactive oxygen ligustrazine oxidative stress damage
- PACS:
- R318；R34
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.07.015
- Abstract:
- Abstract: Objective Age-related macular degeneration (AMD) is one of the main causes of blindness. Retinal pigment epithelium (RPE) cell degeneration induced by oxidative stress plays a key role in the pathogenesis of AMD. The protective mechanism of ligustrazine against oxidative stress damages to human RPE cells is investigated using non-invasive, label-free and highly-sensitive Raman spectroscopy. Methods Before the collection of Raman spectra, RPE cells (ARPE-19) in 2 groups, except control group, were preincubated with 200 μmol/mL H2O2, and 200 μmol/mL ligustrazine was added to the cells in protection group after 24 h. At the end of all interventions, MultiskanGO (Thermo, USA) microplate method was used for determining intracellular reactive oxygen species (ROS) content, and InVia microRaman system for Raman spectrum analysis. Results The ROS levels showed that ligustrazine had a good inhibitory effect on oxidative stress damages induced by H2O2. Raman spectral results demonstrated that the oxidative stress damages to cells were mainly reflected in amino acids. The addition of ligustrazine not only reversed the changing trend of the peak strength ratio of 810 cm-1 and 840 cm-1 assigned to the lipid, but also reduced the peak strength value of each band assigned to amino acids that was significantly increased after H2O2 intervention. Different from the previous laser tweezers Raman spectral results, ROS did not trigger the deamide reaction of amides, but ligustrazine changed the Raman spectral strength assigned to amides. Conclusion Through Raman spectrum analysis on human RPE cells, the potential biomarkers of hydrogen peroxide and ligustrazine are revealed, which laid a foundation for investigating the mechanism of oxidative stress damages to human RPE cells.
Last Update: 2021-07-26