Abstract

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Title:: Effect of different fluid shear stress intensities on expression of Piezo1 protein in MC3T3-E1 osteoblasts

Author(s):: HE Liangzhi; JIANG Jin; LIU Zhongcheng; ZHANG Chengjun; LU Fan; TENG Fei; GENG Bin; XIA Yayi; Department of Orthopaedics, Second Hospital of Lanzhou University Key Laboratory of Bone and Joint Diseases of Gansu Province, Lanzhou 730000, china

Keywords:: Keywords: fluid shear stress osteoblasts Piezo1 protein expression

Abstract:: Abstract: Objective To investigate the expression differences of Piezo1 protein in MC3T3-E1 osteoblasts under different fluid shear stress intensities. Methods The MC3T3-E1 osteoblasts attached to the slides were subjected to different fluid shear stress (0, 3, 6, 9, 12, 15, 18 dyne/cm2) for 45 minutes, and then the expression of Pieoz1 protein in MC3T3-E1 osteoblasts was respectively observed by immunofluorescence and Western Blot. Results The results from mmunofluorescence and Western Blot showed that with the increase of shear stress, the Piezo1 protein expression in MC3T3-E1 osteoblasts increased accordingly, but at 6 dyne/cm2, the expression reached its highest and then decreased gradually, and at 15 or 18 dyne/cm2, the expression kept the same with the blank group, in which the cells were found destroyed and dispersed. Conclusion The increase of fluid shear stress can improve the expression of Piezo1 protein, and when the stress is added to 6 dyne/cm2, the expression reaches its highest point, but a stress greater than that cannot promote the expression of Pizeo1 protein in MC3T3-E1 osteoblasts any more, and instead it damages the cells.