Evaluating the severity of nonalcoholic fatty liver disease in rats by shear wave elastography(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第8期
- Page:
- 955-959
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Evaluating the severity of nonalcoholic fatty liver disease in rats by shear wave elastography
- Author(s):
- ZHOU Qin1; L?Guorong1; 2; GUO Haixin1; LI Jingyun2; ZHANG Shaobo3; CHEN Yongjian1
- 1. Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China 2. School of Clinical?edicine, Quanzhou Medical College, Quanzhou 362000, China 3. Department of Ultrasound, Jinjiang Anhai Hospital, Quanzhou 362000, China
- Keywords:
- Keywords: nonalcoholicai fatty liver disease shear wave elastography biomolecule Youngs modulus
- PACS:
- R365
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.08.007
- Abstract:
- Abstract: Objective To explore the feasibility and influencing factors of shear wave elastography (SWE) technique in assessing the severity of nonalcoholicai fatty liver disease (NAFLD). Methods SD rats were randomly divided into control group (n=40) and experimental group (n=48). The rat models with various degrees of NAFLD severity in experimental group were created by different diets. The mean Youngs modulus (Emean) of the livers was detected at the end of the 1st, 2nd, 3rd, 8th and 12th weeks, and finally the livers were excised for histopathologic evaluation and mRNA expression analysis. Results Emean was positively correlated with NAFLD severity in all rats (r=0.838, P<0.001). There was statistical difference in Emean among different pathological groups (F=113.58, P<0.001). Emean in nonalcoholic steatohepatitis (NASH) and cirrhosis groups was significantly higher than that in normal, simple steatosis and borderline groups (P<0.001). Emean of cirrhosis group was also significantly higher than that of NASH group (P<0.001), and no significant difference was found in Emean among normal group, simple steatosis group and borderline group (P>0.05). The mRNA expressions of MCP-1, TNF-α, type I collagen and α-SMA had significant positive correlations with Emean (r=0.340-0.678, all P<0.001). Among them, MCP-1 (B=0.560, P<0.001) and type I collagen (B=0.429, P<0.001) were independent risk factors for NAFLD. The optimal SWE cutoff values for the diagnosis of NASH and cirrhosis were 6.0 and 9.7 kPa, respectively, with the area under curve of 0.95 and 0.98, sensitivity of 88.9% and 86.5%, and specificity of 100% and 92.7%, respectively. Conclusion SWE technique can be used to assess the severity of NAFLD. MCP-1 and type I collagen are the major biomolecular indicators that affects liver stiffness.
Last Update: 2021-07-30