Fluid shear stress regulates apoptosis of MG-63 osteosarcoma cells through Piezo1(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第8期
- Page:
- 1013-1017
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Fluid shear stress regulates apoptosis of MG-63 osteosarcoma cells through Piezo1
- Author(s):
- MA Hongwu1; 2; MA Chenghua1; 2; ZHANG Yu1; 2; LI Peiwu1; 2
- 1. Emergency Center, Lanzhou University Second Hospital, Lanzhou 730000, China 2. Department of Emergency Surgery, Lanzhou University Second Hospital, Lanzhou 730000, China
- Keywords:
- Keywords: fluid shear stress ion channel osteosarcoma apoptosis
- PACS:
- R34
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.08.018
- Abstract:
- Abstract: Objective To investigate the effect of fluid shear stress (FSS) on Piezo1 and the mechanism of FSS regulating the apoptosis of MG-63 cells. Methods Cells were firstly divided into two groups, namely control group and FSS group. The expressions of caspase3 and Piezo1 were detected by Western Blot after interventions were applied to each group. Then the cells were divided into control group, FSS group, GsMTx4 group and FSS+GsMTx4 group. In FSS+GsMTx4 group, cells were intervened with GsMTx4 first and FSS second. The expression levels of caspase3, caspase9, BAD and Bax were detected after different interventions. Results FSS at 12 dyn/cm2 for 60 min could up-regulate the expression of caspase3 and Piezo1 in MG-63 cells. Besides, FSS could promote the expression levels of caspase3, caspase9, BAD and Bax by up-regulating the expression of Piezo1. Conclusion FSS can promote the apoptosis of MG-63 human osteosarcoma cells through Piezo1 mechanosensitive ion channels. Piezo1 may be a new molecular target for the treatment of osteosarcoma.
Last Update: 2021-07-31