Construction and validation of orthodontic tooth movement model in periostin knockout mice
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《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第10期
- Page:
- 1233-1238
- Research Field:
- 生物材料与力学
- Publishing date:
Info
- Title:
- Construction and validation of orthodontic tooth movement model in periostin knockout mice
- Author(s):
- GUO Wei1; FANG Fuchun2; XU Huiyong2; OUYANG Jun3
- 1. Department of Pathology, Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510515, China; 2. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 3. Department of Anatomy, School of Basic Medicine, Southern Medical University, Guangzhou 510515, China
- Keywords:
- ; periostin; mice model; gene knockout; orthodontic movement
- PACS:
- R318.01
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.10.022
- Abstract:
- Objective To establish the orthodontic tooth movement model in periostin (PN) knockout mice and analyze the related signaling pathways. Methods According to experimental conditions, the enrolled mice were divided into 4 groups, namely C57 mice groups with and without receiving orthodontic force, PN knockout groups with and without receiving orthodontic force, with 5 mice in each group, and the orthodontic force was last for 5 days. After the mice were killed, the upper and lower jaws were scanned with Micro-CT; and the expression levels of related genes in periodontal ligament were detected with RT-PCR. Results Compared with C57 mice, PN knockout mice had obvious alveolar bone resorption and continuous destruction of periodontal ligament. The comparison between two groups of C57 mice showed that the expression levels of PN, focal adhesion kinase (FAK) and TGF-β1 in the periodontal ligament of mice receiving orthodontic force were significantly increased (P<0.05). The comparison between two groups of PN knockout mice showed that the expression levels of FAK and TGF-β1 in the periodontal ligament of mice receiving orthodontic force were remarkably increased (P<0.01). Under the same force condition, the expression level of TGF-β1 in C57 mice was significantly lower than that in PN knockout mice (P<0.05), while the expression level of FAK in C57 mice was obviously increased (P<0.01). Conclusion PN which is an important molecular link in the process of periodontal ligament remodeling under orthodontic action plays an important role in transduction and regulation. TGF-β1--PN--FAK signaling pathway is involved in the process of periodontal ligament remodeling under orthodontic action.
Last Update: 2019-10-30