Abstract

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Title:: Radiosensitizing effect of GO@AgPt nanocomposite on non-small-cell lung cancer

Author(s):: AO Jingsheng1; 2; BAO Zhirong1; QUAN Hong2; 1. Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China; 2. School of Physics and Technology, Wuhan University, Wuhan 430072, China

Keywords:: Keywords: non-small-cell lung cancer; nanocomposite; radiosensitization

Abstract:: Abstract: Objective To prepare a novel GO@AgPt nanocomposite as a new radiotherapy sensitizer and investigate its radiosensitizing effects on non-small-cell lung cancer (NSCLC). Methods GO@AgPt nanocomposite was prepared by an improved hydrothermal method. Transmission electron microscope, atomic force microscope, X-ray photoelectron spectroscopy and Fourier transform infrared spectrometer were utilized to characterize the surface morphology of GO@AgPt nanocomposite and perform an analysis on the chemical compositions. The cytotoxicities of GO@AgPt nanocomposite on NSCLC cells (A549 cells) and human pulmonary microvascular endothelial cells (HPMEC cells) were evaluated by cell counting kit-8 (CCK-8) assay, and the radiosensitization effects of GO@AgPt nanocomposite on A549 cells was determined by colony formation test and flow cytometer. Results GO@AgPt nanocomposite with uniform particle size and stable chemical compositions was successfully prepared. In a certain concentration range, GO@AgPt nanocomposite had a toxic effect on A549 cells, and meanwhile HPMEC cells were not affected. The nanocomposite enhanced the reactive oxygen production in A549 cells, and the combination with X-ray promoted cell apoptosis. Furthermore, the colony formation test showed that GO@AgPt nanocomposite had radiosensitizing effects on A549 cells. Conclusion GO@AgPt nanocomposite is successfully prepared and is proved to be a promising radiotherapy sensitizer for NSCLC.