Synthesis of cell membrane biomimetic liposome-quantum dot nanoprobes(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2018年第7期
- Page:
- 849-854
- Research Field:
- 生物材料与力学
- Publishing date:
Info
- Title:
- Synthesis of cell membrane biomimetic liposome-quantum dot nanoprobes
- Author(s):
- CUI Manman; PAN Weilun; LIU Chunchen; LI Bo
- Department of Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Keywords:
- ; Keywords: nanoprobe; liposomes; quantum dots; biomimetic membrane
- PACS:
- R319；O657.3
- DOI:
- DOI:10.3969/j.issn.1005-202X.2018.07.021
- Abstract:
- Abstract: Objective To synthesize a biomimetic autofluorescence nanoprobe, polyethylene glycol-liposome-quantum dots (PEG-LP-QDs), optimize the synthesis conditions and preliminarily characterize their properties for laying the basis for further application of PEG-LP-QDs in biomedical fields, such as in vivo tracking, tumor detection and multiplex detection. Methods Oil-soluble fluorescent ZnCdSe/ZnS quantum dots were encapsulated by phosphatidylcholine, PEG-phosphatidyl ethanolamine and cholesterol through thin membrane dispersion method for synthesizing PEG-LP-QDs. Transmission electron microscope, nanoparticle tracking technology and fluorescence spectrophotometer detection methods were used to characterize the morphology, diameter size and fluorescence intensity of PEG-LP-QDs, thus further optimizing the synthesis temperature and QDs quantity. Results As the change of synthesis temperature, the results of LP size-changing and QDs fluorescence-decreasing implied that 40 ℃ was a desired temperature. The results of transmission electron microscope and nanoparticle tracking technology proved that PEG-LP-QDs were successfully synthesized. The average diameter size of PEG-LP-QDs was 128 nm. The fluorescence intensity of PEG-LP-QDs reached the maximum when 10 μL 3 mg/mL QDs were encapsulated. Conclusion A novel biomimetic autofluorescence nanoprobe, PEG-LP-QDs, is successfully synthesized, with good biocompatibility and unique luminescent properties. Further work is needed to investigate the application of biomimetic PEG-LP-QDs in biomedical research.
Last Update: 2018-07-24