Effects of loading time of fluid shear stress on expression of matrix metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1 in osteoblasts(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2018年第1期
- Page:
- 102-105
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of loading time of fluid shear stress on expression of matrix metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1 in osteoblasts
- Author(s):
- YANG Quanzeng1; 2; ZHANG Chengjun1; 2; DING Ning1; 2; LI Zhonghao1; 2; XIA Yayi1; 2
- 1. Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou 730000, China; 2. Gansu Key Laboratory of Orthopaedics, Lanzhou 730000, China
- Keywords:
- Keywords: fluid shear stress; MC3T3- E1 cell; matrix metalloproteinase; tissue inhibitors of matrix metalloproteinase
- PACS:
- R35
- DOI:
- DOI:10.3969/j.issn.1005-202X.2018.01.018
- Abstract:
- Abstract: Objective To discuss the variations of the expression of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in osteoblasts with the increasing loading time of fluid shear stress (FSS). Methods MC3T3-E1 cells were subjected to FSS (12 dyn/cm2) using a parallel plate flow system for 0, 15, 30, 45, 60 min. The expression level of MMP-1 and its inhibitor TIMP-1 were detected by Western blotting. Results FSS of 12 dyn /cm2 was applied to the MC3T3-E1 cells cultured in vitro. With the increase of loading time, the expression of MMP-1 was up-regulated, while the expression of TIMP-1 was down-regulated. Forty-five minutes after loading, the expression of MMP-1 was the maximum and that of TIMP-1 reached the minimum. Conclusion Loading 12 dyn /cm2 FSS can promote the expression of MMP-1, and down-regulates the expression of TIMP-1 in MC3T3-E1 cells. The optimal loading time of FSS is 45minutes.
Last Update: 2018-01-25